| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81803-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97637SS | |||
| BSA Free | NBP1-97637-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27336SS | |||
| Azide and BSA Free | NBP2-80800 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72107 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76843-0.025mg | |||
![Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Western-Blot-NB300-619-img0029.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - GR94 in human primary fibroblasts, ER staining. Primary antibody incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4C. Secondary: Donkey anti-rat 647 1:500 2h RT. ICC/IF image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0032.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0028.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Western-Blot-NB300-619-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in Hela Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in NIH-3T3 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse liver tissue.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0021.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse lymph node.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0023.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 (Grp94) in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST (from Product # 37525) for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor, probed with a Grp94 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an HRP-conjugated goat anti-rat IgG (H+L) secondary antibody at a dilution of 1:1000 for 30 minutes at room temperature. Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0026.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Staining on poorly differentiated adenocarcinoma from paraffin-embedded human colon tissue sections.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0027.jpg)
![Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - Analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-Western-Blot-H00004068-M01-img0005.jpg "Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - Analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.")
![Flow Cytometry: SH2D1A Antibody (1C9) [H00004068-M01] - Intracellular staining of human T cells with SH2D1A monoclonal antibody or isotype control antibody, followed by a Polyclonal Goat Anti-Mouse/RPE secondary antibody. Grey filled histogram: Isotype control, empty blue histogram: SH2D1A antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-Flow-Cytometry-H00004068-M01-img0007.jpg)
![Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - SH2D1A monoclonal antibody (M01), clone 1C9 Analysis of SH2D1A expression in Jurkat.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-Western-Blot-H00004068-M01-img0004.jpg)
![ELISA: SH2D1A Antibody (1C9) [H00004068-M01] - Detection limit for recombinant GST tagged SH2D1A is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SH2D1A-Antibody-1C9-ELISA-H00004068-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81803-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81803-img0012.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0025.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0021.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0022.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0023.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0024.jpg)
![Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0001.jpg)
![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining in human thyroid gland and skeletal muscle tissues using NBP1-81802 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining in human thyroid gland and skeletal muscle tissues using NBP1-81802 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81802-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81802-img0011.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81802-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0015.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0018.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0020.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0021.jpg)
![Immunohistochemistry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5 min. at room temperature. Slides were blocked with normal IgG, and incubated for 1 hour with 5ug/ml MAb to BCMA (human) (Vicky-1) in 1% BSA in 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Immunohistochemistry-NBP1-97637-img0007.jpg "Immunohistochemistry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5 min. at room temperature. Slides were blocked with normal IgG, and incubated for 1 hour with 5ug/ml MAb to BCMA (human) (Vicky-1) in 1% BSA in 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.")
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Analysis using the PE conjugate of NBP1-97637. Staining of 10^6 U266 cells using BCMA (human), mAb (Vicky-1) (PE conjugate) at a concentration of 10ug/ml.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Flow-Cytometry-NBP1-97637-img0011.jpg)
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Detection of endogenous human BCMA with MAb to BCMA (human) (Vicky-1). Method: U266 cells (2x10^5) were incubated on ice for 30 min. with 0.2ug of MAb to BCMA (human) (Vicky-1) or an isotype control in 25ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide). The primary antibody was revealed with PAb to Rat IgG (R-PE) and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Flow-Cytometry-NBP1-97637-img0006.jpg)
![Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Analysis using the FITC conjugate of NBP1-97637. Staining of 10^6 Jurkat cells using BCMA (human), mAb (Vicky-1) (FITC conjugate) at a concentration of 50ug/ml.](http://images.novusbio.com/fullsize/BCMA-TNFRSF17-Antibody-Vicky-1-Flow-Cytometry-NBP1-97637-img0010.jpg)
![Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336] - Analysis of IL-21. Full-length recombinant IL-21 protein probed with 1 ug/ml of IL-21 monoclonal antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-21-Antibody-14k5H3-Western-Blot-NBP2-27336-img0002.jpg "Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336] - Analysis of IL-21. Full-length recombinant IL-21 protein probed with 1 ug/ml of IL-21 monoclonal antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Western-Blot-NBP2-45868-img0005.jpg "Western Blot: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7.")
![Immunohistochemistry-Paraffin: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Paraffin-embedded Carcinoma of Human prostate tissue using anti-SLAMF7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-Paraffin-NBP2-45868-img0006.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0001.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0002.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0003.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0004.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF/BLyS/TNFSF13B antibody at 1 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-271120242010580.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 ug of lysates per lane.Antibodies: BAFF/BLyS/TNFSF13B (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285892.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF/BLyS/TNFSF13B with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285848.gif)
![Western Blot: CXCR5 Antibody (JB11-40) [NBP2-75460] - Western blot analysis of CXCR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: Raji cell lysate](http://images.novusbio.com/fullsize/CXCR5-Antibody-JB11-40-Western-Blot-NBP2-75460-img0006.jpg "Western Blot: CXCR5 Antibody (JB11-40) [NBP2-75460] - Western blot analysis of CXCR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: Raji cell lysate")
![Immunohistochemistry-Paraffin: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of paraffin-embedded rat kidney tissue using anti-CXCR5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CXCR5-Antibody-JB11-40-Immunohistochemistry-Paraffin-NBP2-75460-img0004.jpg)
![Flow Cytometry: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of Daudi cells with CXCR5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CXCR5-Antibody-JB11-40-Flow-Cytometry-NBP2-75460-img0001.jpg)
![Immunohistochemistry-Paraffin: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of paraffin-embedded human tonsil tissue using anti-CXCR5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CXCR5-Antibody-JB11-40-Immunohistochemistry-Paraffin-NBP2-75460-img0002.jpg)
![Immunohistochemistry-Paraffin: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of paraffin-embedded mouse heart tissue using anti-CXCR5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CXCR5-Antibody-JB11-40-Immunohistochemistry-Paraffin-NBP2-75460-img0003.jpg)