| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24917SS | |||
| Azide and BSA Free | NBP2-77073 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56563SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18816SS | |||
| Azide and BSA Free | NBP3-18817 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86095-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7463APC | |||
| Biotin | NB7463B | |||
| DyLight 350 | NB7463UV | |||
| DyLight 405 | NB7463V | |||
| DyLight 488 | NB7463G | |||
| DyLight 550 | NB7463R | |||
| DyLight 594 | NB7463DL594 | |||
| DyLight 650 | NB7463C | |||
| DyLight 680 | NB7463FR | |||
| DyLight 755 | NB7463IR | |||
| FITC | NB7465 | |||
| HRP | NB7466 | |||
| Janelia Fluor 525 | NB7463JF525 | |||
| Janelia Fluor 669 | NB7463JF669 | |||
| PE | NB7463PE | |||
| PerCP | NB7463PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47672-0.1mg | |||
| IHC-Prediluted | NBP2-48136 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56878SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48122 | |||
| Azide and BSA Free | NBP3-11419 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7546APC | |||
| Biotin | NB7546B | |||
| DyLight 350 | NB7546UV | |||
| DyLight 405 | NB7546V | |||
| DyLight 488 | NB7546G | |||
| DyLight 550 | NB7546R | |||
| DyLight 594 | NB7546DL594 | |||
| DyLight 650 | NB7546C | |||
| DyLight 680 | NB7546FR | |||
| DyLight 755 | NB7546IR | |||
| FITC | NB7548 | |||
| HRP | NB7549 | |||
| Janelia Fluor 525 | NB7546JF525 | |||
| Janelia Fluor 669 | NB7546JF669 | |||
| PE | NB7546PE | |||
| PerCP | NB7546PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB7547-0.5mg | |||
| Allophycocyanin | NB7546APC | |||
| Biotin | NB7546B | |||
| DyLight 350 | NB7546UV | |||
| DyLight 405 | NB7546V | |||
| DyLight 488 | NB7546G | |||
| DyLight 550 | NB7546R | |||
| DyLight 594 | NB7546DL594 | |||
| DyLight 650 | NB7546C | |||
| DyLight 680 | NB7546FR | |||
| DyLight 755 | NB7546IR | |||
| FITC | NB7548 | |||
| PE | NB7546PE | |||
| PerCP | NB7546PCP | |||
![Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n=4 subjects). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Flow-Cytometry-NBP2-24917-img0018.jpg "Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n=4 subjects). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.")
![Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Expression of TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR8 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR8. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Flow-Cytometry-NBP2-24917-img0021.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - All the cell lines expressed mRNA for TLR8 (Figure 1) and displayed a strong expression of TLR8 protein (Figure 2). Lysates of human intestinal tissue and mouse splenocytes were used as positive controls. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Western-Blot-NBP2-24917-img0022.jpg)
![Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Immunohistochemistry-Paraffin-NBP2-24917-img0005.jpg)
![Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - HEK cells expressing TLR8 were treated with ssRNA40-Alexa 546 followed by fixation, permeablization and staining for TLR8 using anti-TLR8 Alexa 488 conjugate or isotype control IgG1/Kappa Alexa 488. TLR8 detection is shown along the horizontal axis, and ssRNA40 Alexa 546 detection is shown along the vertical axis. Direct excitation (488 and 561 nm lasers) of both fluorophores (Alexa 488 and Alexa 546) allowed quantitation of cells staining positive for TLR8 (anti-TLR8-Alexa488) and cells that had taken up ssRNA40-Alexa 546. Single-stranded RNA40 binds TLR8 (flow cytometry FRET assay). A representative experiment is shown from three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Flow-Cytometry-NBP2-24917-img0019.jpg)
![Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Western-Blot-NBP2-24917-img0006.jpg)
![Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/TLR8-Antibody-44C143-Simple-Western-NBP2-24917-img0023.jpg)
![Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific band at an apparent molecular mass of 37 kDa was observed. Image from verified customer review.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Western-Blot-NBP2-24683-img0014.jpg "Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific band at an apparent molecular mass of 37 kDa was observed. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - RH-30 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with STING/TMEM173 Antibody at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha Tubulin Antibody (DM1A) (NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24683-img0017.jpg)
![Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml STING/TMEM173 Antibody in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Western-Blot-NBP2-24683-img0011.jpg)
![Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Human colon cancer tissue section using STING/TMEM173 Antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated very weak cytoplasmic staining in columnar epithelial cells with a very strong signal in the secretory/goblet cells.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunohistochemistry-Paraffin-NBP2-24683-img0006.jpg)
![Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING/TMEM173 Antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunohistochemistry-Paraffin-NBP2-24683-img0007.jpg)
![Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on U937 cells with NBP2-24683AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Flow-Cytometry-NBP2-24683-img0020.jpg)
![Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody NBP2-24683AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody---BSA-Free-Flow-Cytometry-NBP2-24683-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - HT-29 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with STING/TMEM173 Antibody at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24683-img0015.jpg)
![Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Flow-Cytometry-NBP2-24683-img0012.jpg)
![Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on RH30 cells with STING/TMEM173 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-Flow-Cytometry-NBP2-24683-img0016.jpg)
![Expression of STING and cGAS mRNA and protein in adipocytes. (A) Venn diagram summarizing the number of equally and differently expressed mRNA transcripts of young and adult mouse iAT. A gene network associated with Sting1 was equally expressed by young and adult iAT. A protein–protein interaction map, generated by STRING [30] is shown below the Venn diagram. Extended analysis presented in [24]. (B) Immunofluorescence of in vitro cultured adipocytes from young and adult mouse iAT; nc: nucleus, scale bar 20 μm. (C) Immunostaining of STING and cGAS proteins in the iAT of young mice, showing a region containing both multilocular and unilocular adipocytes. Arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 50 μm. (D) Top: Expression of STING1 and CGAS mRNA in human inguinal and abdominal adipose tissue specimens. Linear regression analysis indicates a significant positive correlation between STING1 and CGAS mRNA levels. Each data point represents one tissue donor patient. Bottom: Correlation of donor age and the adipose tissue expression levels of STING1 and CGAS. (E) Immunohistochemistry of STING and cGAS proteins in human adipose tissue, collected from the inguinal-low abdominal region. Nineteen-month-old male infant; arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 25 μm. Inlet shows nuclear STING labeling of an in vitro cultured human adipocyte. Scale bar: 20 μm. (F) Body mass index z-score (BMI z-score) and BMI standard deviation score (BMI-SDS) of adipose tissue donors involved in this study. Correlation of BMI z-score with adipose tissue STING1 and CGAS mRNA levels. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37830559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24683_rabbit-polyclonal-sting-tmem173-antibody-immunocytochemistry-immunofluorescence-13220261944235.jpg)
![Expression of STING and cGAS mRNA and protein in adipocytes. (A) Venn diagram summarizing the number of equally and differently expressed mRNA transcripts of young and adult mouse iAT. A gene network associated with Sting1 was equally expressed by young and adult iAT. A protein–protein interaction map, generated by STRING [30] is shown below the Venn diagram. Extended analysis presented in [24]. (B) Immunofluorescence of in vitro cultured adipocytes from young and adult mouse iAT; nc: nucleus, scale bar 20 μm. (C) Immunostaining of STING and cGAS proteins in the iAT of young mice, showing a region containing both multilocular and unilocular adipocytes. Arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 50 μm. (D) Top: Expression of STING1 and CGAS mRNA in human inguinal and abdominal adipose tissue specimens. Linear regression analysis indicates a significant positive correlation between STING1 and CGAS mRNA levels. Each data point represents one tissue donor patient. Bottom: Correlation of donor age and the adipose tissue expression levels of STING1 and CGAS. (E) Immunohistochemistry of STING and cGAS proteins in human adipose tissue, collected from the inguinal-low abdominal region. Nineteen-month-old male infant; arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 25 μm. Inlet shows nuclear STING labeling of an in vitro cultured human adipocyte. Scale bar: 20 μm. (F) Body mass index z-score (BMI z-score) and BMI standard deviation score (BMI-SDS) of adipose tissue donors involved in this study. Correlation of BMI z-score with adipose tissue STING1 and CGAS mRNA levels. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37830559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24683_rabbit-polyclonal-sting-tmem173-antibody-immunohistochemistry-132202619354822.jpg)
![Expression of STING and cGAS mRNA and protein in adipocytes. (A) Venn diagram summarizing the number of equally and differently expressed mRNA transcripts of young and adult mouse iAT. A gene network associated with Sting1 was equally expressed by young and adult iAT. A protein–protein interaction map, generated by STRING [30] is shown below the Venn diagram. Extended analysis presented in [24]. (B) Immunofluorescence of in vitro cultured adipocytes from young and adult mouse iAT; nc: nucleus, scale bar 20 μm. (C) Immunostaining of STING and cGAS proteins in the iAT of young mice, showing a region containing both multilocular and unilocular adipocytes. Arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 50 μm. (D) Top: Expression of STING1 and CGAS mRNA in human inguinal and abdominal adipose tissue specimens. Linear regression analysis indicates a significant positive correlation between STING1 and CGAS mRNA levels. Each data point represents one tissue donor patient. Bottom: Correlation of donor age and the adipose tissue expression levels of STING1 and CGAS. (E) Immunohistochemistry of STING and cGAS proteins in human adipose tissue, collected from the inguinal-low abdominal region. Nineteen-month-old male infant; arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 25 μm. Inlet shows nuclear STING labeling of an in vitro cultured human adipocyte. Scale bar: 20 μm. (F) Body mass index z-score (BMI z-score) and BMI standard deviation score (BMI-SDS) of adipose tissue donors involved in this study. Correlation of BMI z-score with adipose tissue STING1 and CGAS mRNA levels. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37830559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24683_rabbit-polyclonal-sting-tmem173-antibody-immunohistochemistry-132202619213316.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0016.jpg "Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.")
![Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NB100-56563-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR1-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NB100-56563-img0014.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Expression of TLR1 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR1 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR1. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0015.jpg)
![Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563] - analysisof human small intestine using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TLR1-Antibody-Immunohistochemistry-Paraffin-NB100-56563-img0009.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells alone, green represents isotype controlwas used for this test.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0013.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Intracellular analysis of TLR1 AF647 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents TLR1 antibody.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0012.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56152-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NB100-56152] - Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NB100-56152] - Representative images from the immunofluorescence method (x400). HepG2 and SMMC-7721 cells were incubated with or without Evo (0, 0.5, and 1 uM) for 24 h to detect levels of NOD1 and p-P65. Values are means and standard errors of three separate experiments (* p < 0.05 and ** p < 0.01 versus control). Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56152-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56152-img0004.jpg)
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56152] - Section of human sebaceous gland (A) and colon (B) stained for NOD1 expression using NB100-56152 at 1:2000. Hematoxylin counterstain. 10X magnification.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56152-img0002.jpg)
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56152] - Sections of human colon (A, A1) and pancreas (B, B1) stained for NOD1 expression using a DAB chromogen and Hematoxylin counterstain. Magnification: A (20X), A1 (40X). B (10X), B1 (40X).](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56152-img0001.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo induces apoptotic cell death of HepG2 & SMMC-7721 cells via the NOD1-mediated apoptotic pathway in vitro. HepG2 & SMMC-7721 cells were treated with 10 µg/mL IE-DAP for 2 h before exposure to 1 µM Evo. Apoptosis (A), cycle arrest (B), apoptosis-related protein levels (C) & the proteins’ levels of NOD1 pathway (D) in treated & untreated cells were measured by 5-ethynyl-2′-deoxyuridine (EdU) (×40), cellular propidium iodide (PI) fluorescence & Western blot methods. The percentage of proliferating cells (EdU+) was quantitated using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Values are means & standard errors of three separate experiments (* p < 0.05 & ** p < 0.01 versus control, #p < 0.05 & ##p < 0.01 versus Evo). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-31020241534319.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. (C) Representative images from the immunofluorescence method. Expression of NOD1 (×400) & p-P65 (×200) in tumor tissues was analyzed by the immunofluorescence method. Values are means & standard errors of five separate experiments (* p < 0.05 & ** p < 0.01 versus control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-310202415175228.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo decreases expression of NOD1 resulting in suppression of nuclear factor-kappa B (NF-kappa B) & mitogen-activated protein kinase (MAPK) activation in vitro. (A) Expression of NOD1 in normal hepatocyte HL-7702, HepG2, & SMMC-7721 cells were detected by qRT-PCR & Western blot assays. (B) HepG2 & SMMC-7721 cells were incubated with 1 µM Evo for 0, 3, 6, & 12 h. The Western blot assay was performed to detect levels of proteins in the NOD1 pathway. (C) Representative images from the immunofluorescence method (×400). HepG2 & SMMC-7721 cells were incubated with or without Evo (0, 0.5, & 1 µM) for 24 h to detect levels of NOD1 & p-P65. Values are means & standard errors of three separate experiments (* p < 0.05 & ** p < 0.01 versus control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-310202415293347.jpg)
![Western Blot: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Western blot shows cell lysates K562, THP-1, C2C12, and C6. Membrane was probed with 1 ug/mL of (Catalog # NBP3-18816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Western-Blot-NBP3-18816-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed U937 human myeloid leukaemia cell line but is not detected in Daudi cell line using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # NBP3-18816) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunocytochemistry-Immunofluorescence-NBP3-18816-img0002.jpg)
![Immunohistochemistry: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunohistochemistry-NBP3-18816-img0001.jpg)
![Simple Western: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Simple Western Lane view shows K562 and THP-1 whole cell lysate (WCL). A specific band was detected for hSTING/TMEM173 antibody (NBP3-18816) at approximately 44 kDa (as indicated) using 10 ug/mL of hSTING/TMEM173 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Simple-Western-NBP3-18816-img0004.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Analysis in human duodenum and skeletal muscle tissues. Corresponding PIGR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Analysis in human duodenum and skeletal muscle tissues. Corresponding PIGR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0014.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0012.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0013.jpg)
![Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-86095] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/pIgR-Antibody-Immunohistochemistry-Paraffin-NBP1-86095-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-Kappa-Light-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7463-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.")
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0006.jpg "Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).")
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0002.jpg)
![Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). conjugated with APC (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Flow-Cytometry-NBP2-45316-img0003.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0001.jpg)
![Immunofluorescence: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with CF594 (Cyan).The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunofluorescence-NBP2-45316-img0004.jpg)
![Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56878-img0003.jpg "Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.")
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56878] - analysis of human pancreas using NOD1 antibody at 1:100.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56878-img0002.jpg)
![Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Western-Blot-NBP2-45312-img0005.jpg "Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).")
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Immunohistochemistry-Paraffin-NBP2-45312-img0004.jpg)
![Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM288).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Flow-Cytometry-NBP2-45312-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (SPM288).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Immunohistochemistry-Paraffin-NBP2-45312-img0001.jpg)
![Immunofluorescence: HLA DRB1 Antibody (SPM288) [NBP2-45312] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with APC (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Immunofluorescence-NBP2-45312-img0003.jpg)
![Western Blot: Goat anti- Mouse, Human Kappa Light Chain Secondary Antibody [NB7546] - Analysis in 1. c whole cell lysate, 2. ASPC1 tumor lysate. Image from verified customer review. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti--Mouse--Human-Kappa-Light-Chain-Secondary-Antibody-Western-Blot-NB7546-img0001.jpg "Western Blot: Goat anti- Mouse, Human Kappa Light Chain Secondary Antibody [NB7546] - Analysis in 1. c whole cell lysate, 2. ASPC1 tumor lysate. Image from verified customer review. Image using the HRP format of this antibody.")
![Western Blot: Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] [NB7549] - Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-Kappa-Light-Chain-Secondary-Antibody-[HRP]-Western-Blot-NB7549-img0001.jpg "Western Blot: Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] [NB7549] - Image from verified customer review.")