Goat anti-Mouse Kappa Light Chain Secondary Antibody

Western Blot: Goat anti- Mouse, Human Kappa Light Chain Secondary Antibody [NB7546] - Analysis in 1. c whole cell lysate, 2. ASPC1 tumor lysate. Image from verified customer review. Image using the HRP format of this antibody.

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Mouse Kappa Light Chain Secondary Antibody Summary

• Immunogen: Mouse Kappa Light Chain
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all mouse immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGKC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000 - 1:30000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Mouse Kappa Light Chain Secondary Antibody

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kappa Light Chain Antibody (NB7546)(8)

Publications using NB7546

• Suzuki M, Kuromi H, Shindo M et al. A Drosophila model of diabetic neuropathy reveals a role of proteasome activity in the glia iScience 2023-05-01 [PMID: 37378316] (Western Blot)
  
  Details:
  WB 1:10000
• Bauer E, Schlederer M, Scheicher R et al. Cooperation of ETV6/RUNX1 and BCL2 enhances immunoglobulin production and accelerates glomerulonephritis in transgenic mice. Oncotarget. [PMID: 26919255]
  
  Details:
  Citation using the HRP form of this antibody.
• Miyamoto S, Iwamoto R, Furuya A et al. A Novel Anti-Human HB-EGF Monoclonal Antibody with Multiple Anti-Tumor Mechanisms Against Ovarian Cancer Cells. Clinical Cancer Research: An Official Journal of the American Association for Cancer Research. [PMID: 21918176]
  
  Details:
  Citation using the FITC form of this antibody.
• Saitoh Y, Terada N, Saitoh S et al. Three-dimensional reconstruction of living mouse liver tissues using cryotechniques with confocal laser scanning microscopy. J Electron Microsc (Tokyo) 2010-01-01 [PMID: 20709827]
• Papic N, Hunn JP, Pawlowski N et al. Inactive and active states of the interferon-inducible resistance GTPase, Irga6, in vivo. J Biol Chem 2008-11-01 [PMID: 18784077]
• Mehenni H, Lin-Marq N, Buchet-Poyau K et al. LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Hum Mol Genet. [PMID: 15987703]
  
  Details:
  Citation using the HRP form of this antibody.
• Tomomori-Sato C, Sato S, Parmely TJ et al. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. J Biol Chem 2004-02-01 [PMID: 14638676]
• Sato S, Tomomori-Sato C, Banks CA et al. A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. J Biol Chem 2003-12-01 [PMID: 14576168]

Bioinformatics

• Gene Symbol: IGKC