| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57554-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85978-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18540-25ul | |||
| Azide and BSA Free | NBP3-44162 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89446-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18574-25ul | |||
| Azide and BSA Free | NBP3-44163 | |||
![Western Blot: PIASy Antibody [NB600-1318] - Western blot analysis of PIASg in Jurkat cell lysate using NB600-1318 at 2 ug/ml.](http://images.novusbio.com/fullsize/PIASy-Antibody-Western-Blot-NB600-1318-img0001.jpg "Western Blot: PIASy Antibody [NB600-1318] - Western blot analysis of PIASg in Jurkat cell lysate using NB600-1318 at 2 ug/ml.")
![Western Blot: PIASy Antibody [NB600-1318] - Analysis of PIASin A) human liver, B) mouse liver, C) rat liver and D) human Jurkat lysate using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/PIASy-Antibody-Western-Blot-NB600-1318-img0002.jpg)
![Western Blot: SMURF2 Antibody [NBP2-57554] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Western-Blot-NBP2-57554-img0008.jpg "Western Blot: SMURF2 Antibody [NBP2-57554] - Analysis in human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: SMURF2 Antibody [NBP2-57554] - Staining of human cell line BJ shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57554-img0001.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0007.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0004.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0005.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0006.jpg)
![Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742] - Analysis of extracts of Mouse testis, using NAT10 antibody (NBP3-15742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NAT10-Antibody-5Z3E5-Western-Blot-NBP3-15742-img0005.jpg "Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742] - Analysis of extracts of Mouse testis, using NAT10 antibody (NBP3-15742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-52202517311058.jpg)
![Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-5220251734583.jpg)
![Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human colon using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-5220251734527.jpg)
![Western Blot: NAT10 Antibody (5Z3E5) [NAT10] - Western blot analysis of various lysates using NAT10 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-5220251730490.jpg)
![Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-52202517304920.jpg)
![Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-52202517344229.jpg)
![Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15742_rabbit-nat10-mab-5z3e5-52202517361243.jpg)
![Western Blot: SMURF2 Antibody [NBP2-81916] - Analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Western-Blot-NBP2-81916-img0001.jpg "Western Blot: SMURF2 Antibody [NBP2-81916] - Analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 ug/ml.")
![Immunohistochemistry: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202420285849.gif)
![Immunohistochemistry: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202419381720.jpg)
![Immunocytochemistry/ Immunofluorescence: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 ug/ml.Green: SMURF2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-271120241958449.gif)
![Immunocytochemistry/ Immunofluorescence: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/ml.Green: SMURF2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202420432386.gif)
![Immunocytochemistry/ Immunofluorescence: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202419475814.jpg)
![Immunohistochemistry: SMURF2 Antibody - BSA Free [NBP2-81916] - Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81916_rabbit-polyclonal-smurf2-antibody-2711202419514042.gif)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0013.jpg "Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Stain ing of human tonsil shows low positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0006.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0007.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0008.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0009.jpg)
![Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Western-Blot-NBP3-18540-img0007.jpg "Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Analysis in human cell line U-2 OS.")
![Immunocytochemistry/Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of U2-OS cells using the Anti-TFE3 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Immunocytochemistry-Immunofluorescence-NBP3-18540-img0009.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Immunohistochemistry-Paraffin-NBP3-18540-img0006.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human kidney cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Immunohistochemistry-Paraffin-NBP3-18540-img0002.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Immunohistochemistry-Paraffin-NBP3-18540-img0003.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Immunohistochemistry-Paraffin-NBP3-18540-img0004.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12822-Immunohistochemistry-Paraffin-NBP3-18540-img0005.jpg)
![Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-89446] - Staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/KIF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89446-img0005.jpg)
![Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PIASy-Antibody-Western-Blot-NBP1-31215-img0008.jpg "Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PIASy Antibody [NBP1-31215] - Paraformaldehyde-fixed HeLa, using PIASy antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31215-img0003.jpg)
![Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Mouse colon. PIASy antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunohistochemistry-NBP1-31215-img0006.jpg)
![Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Paraffin-embedded DLD1 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunohistochemistry-Paraffin-NBP1-31215-img0004.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0006.jpg "Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human kidney cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0001.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0002.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0003.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0004.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Staining of human kidney shows moderate to strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0005.jpg)
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12810) [NBP3-18574] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-CL12810-Immunohistochemistry-Paraffin-NBP3-18574-img0007.jpg)
![Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Western-Blot-NBP2-32263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0004.jpg)
![Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0003.jpg)
![Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by another rabbit anti-NAT10 antibody. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0005.jpg)
![Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of various cell lines, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-7V8M1-Western-Blot-NBP3-15276-img0002.jpg "Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of various cell lines, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Transcription factor E3 Rabbit mAb (NBP3-15276) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-7V8M1-Immunohistochemistry-Paraffin-NBP3-15276-img0001.jpg)
![Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of HeLa cells, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Transcription-factor-E3-Antibody-7V8M1-Western-Blot-NBP3-15276-img0003.jpg)
![Immunohistochemistry: Transcription factor E3 Antibody (1O9E9) [Transcription factor E3] - Immunohistochemistry analysis of paraffin-embedded Human acinar soft tissue sarcoma using Transcription factor E3 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15276_rabbit-transcription-factor-e3-mab-1o9e9-5220251718978.jpg)
![Western Blot: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Western blot analysis of Transcription factor E3 on different lysates with Rabbit anti-Transcription factor E3 antibody (NBP3-33005) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: C2C12 cell lysate (20 ug/Lane) Lane 4: Mouse testis tissue lysate (40 ug/Lane) Lane 5: A-172 cell lysate (30 ug/Lane) Lane 6: NIH/3T3 cell lysate (30 ug/Lane) Lane 7: C6 cell lysate (30 ug/Lane) Lane 8: PC-12 cell lysate (30 ug/Lane) Predicted band size: 61 kDa Observed band size: 70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33005) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33005_rabbit-transcription-factor-e3-mab-je60-60-288202412493486.jpg "Western Blot: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Western blot analysis of Transcription factor E3 on different lysates with Rabbit anti-Transcription factor E3 antibody (NBP3-33005) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: C2C12 cell lysate (20 ug/Lane) Lane 4: Mouse testis tissue lysate (40 ug/Lane) Lane 5: A-172 cell lysate (30 ug/Lane) Lane 6: NIH/3T3 cell lysate (30 ug/Lane) Lane 7: C6 cell lysate (30 ug/Lane) Lane 8: PC-12 cell lysate (30 ug/Lane) Predicted band size: 61 kDa Observed band size: 70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33005) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Transcription factor E3 antibody (NBP3-33005) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33005) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33005_rabbit-transcription-factor-e3-mab-je60-60-298202413585561.jpg)
![Flow Cytometry: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Flow cytometric analysis of U-2 OS cells labeling Transcription factor E3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33005, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33005_rabbit-transcription-factor-e3-mab-je60-60-392024884611.jpg)