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![Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).](http://images.novusbio.com/fullsize/Cytomegalovirus-Antibody-DDG9-and-CCH2-Immunohistochemistry-Paraffin-NBP1-22532-img0004.jpg "Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).")
![Immunohistochemistry: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - Human Placenta CMV. HIER sodium citrate 6.0 - 30 minutes 1:10 dilution. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Cytomegalovirus-Antibody-DDG9-and-CCH2-Immunohistochemistry-NBP1-22532-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).](http://images.novusbio.com/fullsize/Cytomegalovirus-Antibody-DDG9-and-CCH2-Immunohistochemistry-Paraffin-NBP1-22532-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded human salivary glands (CMV infected) using Cytomegalovirus antibody (clone DDG9 and CCH2).](http://images.novusbio.com/fullsize/Cytomegalovirus-Antibody-DDG9-and-CCH2-Immunohistochemistry-Paraffin-NBP1-22532-img0003.jpg)
![Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-11E3-Flow-Cytometry-NBP1-97662-img0001.jpg "Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.")
![Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) Alexa Fluor® 700.](http://images.novusbio.com/fullsize/CD46-Antibody-MEM-258-Flow-Cytometry-NB500-301-img0006.jpg "Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) Alexa Fluor® 700.")
![Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) PE](http://images.novusbio.com/fullsize/CD46-Antibody-MEM-258-Flow-Cytometry-NB500-301-img0001.jpg)
![Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) biotin](http://images.novusbio.com/fullsize/CD46-Antibody-MEM-258-Flow-Cytometry-NB500-301-img0002.jpg)
![Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD46-Antibody-MEM-258-Flow-Cytometry-NB500-301-img0003.jpg)
![Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) APC.](http://images.novusbio.com/fullsize/CD46-Antibody-MEM-258-Flow-Cytometry-NB500-301-img0004.jpg)
![Flow Cytometry: CD46 Antibody (MEM-258) [NB500-301] - Staining of human peripheral blood cells with anti-CD46 (MEM-258) FITC.](http://images.novusbio.com/fullsize/CD46-Antibody-MEM-258-Flow-Cytometry-NB500-301-img0005.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Lane 1: Wild-type HepG2 whole cell lysate (10 ug).Lane 2: CD206 knockdown HepG2 whole cell lysate (10 ug).Lane 3: CD206 knockdown HepG2 whole cell lysate (10 ug).NBP2-66956 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0003.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of MMR/CD206/Mannose Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: D3 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0005.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human lung tissue lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0006.jpg)
![Western Blot: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: 293T cell lysateLane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Western-Blot-NBP2-66956-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MMR/CD206/Mannose Receptor Antibody (JF0953) [NBP2-66956] - Staining Mannose Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-JF0953-Immunocytochemistry-Immunofluorescence-NBP2-66956-img0002.jpg)
![Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Human classic Hodgkin's lymphoma stained with OX40 (clone Ber-ACT35) at dilution 1:50, pH9 heat-induced epitope retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-Ber-ACT35-Immunohistochemistry-Paraffin-NBP2-62202-img0003.jpg "Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Human classic Hodgkin's lymphoma stained with OX40 (clone Ber-ACT35) at dilution 1:50, pH9 heat-induced epitope retrieval. Image from verified customer review.")
![Flow Cytometry: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Staining of human PMA + ionomycin activated PBMC with anti-human CD134 (Ber-ACT35) PE.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-Ber-ACT35-Flow-Cytometry-NBP2-62202-img0002.jpg)
![Flow (Cell Surface): OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Surface staining of human PHA-activated leukocytes with anti-human CD134 (Ber-ACT35) purified, GAM APC.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-Ber-ACT35-Flow-Cell-Surface-NBP2-62202-img0001.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0009.jpg "Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.")
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine Antibody at 1:1,000 o/n at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0003.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none. Image from the Biotin version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0006.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0007.jpg)
![Western Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Texas Red swine secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none. Image from the Texas Red version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Western-Blot-NBP1-72700-img0008.jpg)
![Dot Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Dot Blot of Goat anti-Porcine IgG (H+L) Secondary Antibody [Alkaline Phosphatase]. Antigen: Swine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72700-img0004.jpg)
![Dot Blot: Goat anti-Porcine IgG (H+L) Secondary Antibody [Unconjugated] [NBP1-72700] - Load: 200 ug followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Fluorescien swine secondary antibody at 1:1,000 for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Porcine-IgG-H+L-Secondary-Antibody-Unconjugated-Dot-Blot-NBP1-72700-img0005.jpg)
![Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72745-img0008.jpg "Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - Immunocytochemistry/Immunofluorescence: Donkey anti-Sheep IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-73002] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72745-img0003.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - ELISA results of purified Polyclonal Donkey anti-Sheep IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) tested against Sheep IgG. Each well was coated in duplicate with 1.0ug of antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer. Using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72745-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72745-img0009.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - ELISA Results of Donkey anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Sheep IgG Mx8. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Red Line], Chicken IgG [Green Line], Guinea Porcine IgG [Blue Line], Hamster IgG [Purple Line], Equine IgG, Human IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution of Sheep IgG MX8 is 1:49,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72745-img0010.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB120-7056-img0003.jpg "Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NB120-7056-img0007.jpg)
![Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB120-7056-img0001.jpg)
![ELISA: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - ELISA results of purified Donkey anti-Mouse IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) tested against purified Mouse IgG. (Blue Line). Each well was coated in duplicate with 1.0 ug of antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer. Using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NB120-7056-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7056] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NB120-7056-img0008.jpg)
![Western Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Lane 1: Molecular Weigh Ladder. Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Horse Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Loading: 50ng IgG, Serum at 1:8 dilution. Incubated in blocking buffer for 30 min at RT. Secondary Antibody: NB120-7139. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NB120-7134-img0004.jpg "Western Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Lane 1: Molecular Weigh Ladder. Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Horse Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Loading: 50ng IgG, Serum at 1:8 dilution. Incubated in blocking buffer for 30 min at RT. Secondary Antibody: NB120-7139. Image from the HRP version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Immunocytochemistry/Immunofluorescence: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72854]](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NB120-7134-img0005.jpg)
![Dot Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Guinea Pig IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Visualized using Alkaline Phosphatase Substrate for 30 seconds at RT. Image from the Alkaline Phosphatase version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NB120-7134-img0001.jpg)
![Dot Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Dots are Guinea Pig IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Guinea Pig IgG Antibody FITC at 1ug/mL in blocking buffer for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NB120-7134-img0002.jpg)
![ELISA: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NB120-7134-img0009.jpg)
![ELISA: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB120-7134] - ELISA Results of Goat anti-Guinea Porcine IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Guinea Porcine MX10. Each well was coated in duplicate with 1.0 ug of Guinea Porcine IgG. The working dilution is 1:51,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Donkey Anti-Goat IgG HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NB120-7134-img0010.jpg)
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody.Lane 1: Rabbit F(c).Load: 50 ng per lane.Primary antibody: Rabbit F(c) antibody at 1:1,000 overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 kDa, 25 kDa for Rabbit F(c).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Western-Blot-NBP2-60661-img0013.jpg "Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody.Lane 1: Rabbit F(c).Load: 50 ng per lane.Primary antibody: Rabbit F(c) antibody at 1:1,000 overnight at 4C.Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 kDa, 25 kDa for Rabbit F(c).")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Immunocytochemistry/Immunofluorescence: [DyLight 488] [NBP1-72943]](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60661-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. Image using the Rhodamine form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Western-Blot-NBP2-60661-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Fluorophore-linked immunosorbent assay: Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0009.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0010.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG Fc Secondary Antibody [NBP2-60661] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-Fc-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60661-img0011.jpg)
![Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29331-img0001.jpg "Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).")
![Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Western-Blot-NB100-56654-img0006.jpg "Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56654] - Analysis of human pancreas using TRAF3 antibody at 1:50.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NB100-56654-img0005.jpg)