| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97181 | |||
| FITC | NBP1-97161 | |||
| HRP | NBP1-97195 | |||
| Rhodamine | NBP1-97117 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97117 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0470A488-100 | |||
| Alexa Fluor 546 | DDX0470A546-100 | |||
| Alexa Fluor 647 | DDX0470A647-100 | |||
| Biotin | DDX0470B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31372SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-87047SS | |||
| Azide and BSA Free | NBP2-80605 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97172 | |||
| FITC | NBP1-97150 | |||
| HRP | NBP1-97206 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0340A488-100 | |||
| Alexa Fluor 546 | DDX0340A546-100 | |||
| Alexa Fluor 647 | DDX0340A647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13159SS | |||
![SDS-Page: Human IgA Isotype Control [NBP1-97039] - SDS-PAGE of Human IgA Isotype control.Lane 1: Human IgA Isotype control, Reduced [5.0ug].Lane 2: Human IgA Isotype control, Reduced [1.0ug].Lane 3: Opal Prestained Molecular Weight Marker.Lane 4: Human IgA Isotype control, Non-Reduced [1.0ug].Lane 5: Human IgA Isotype control, Non-Reduced [5.0ug].4-20% Gel, Coomassie Blue Stained. Expected MW: ~160kDa Non-Reduced, ~55kDa, and 23kDa Reduced. Additional bands: monomer, dimer and trimer.](http://images.novusbio.com/fullsize/Human IgA Isotype Control-SDS-Page-NBP1-97039-img0003.jpg "SDS-Page: Human IgA Isotype Control [NBP1-97039] - SDS-PAGE of Human IgA Isotype control.Lane 1: Human IgA Isotype control, Reduced [5.0ug].Lane 2: Human IgA Isotype control, Reduced [1.0ug].Lane 3: Opal Prestained Molecular Weight Marker.Lane 4: Human IgA Isotype control, Non-Reduced [1.0ug].Lane 5: Human IgA Isotype control, Non-Reduced [5.0ug].4-20% Gel, Coomassie Blue Stained. Expected MW: ~160kDa Non-Reduced, ~55kDa, and 23kDa Reduced. Additional bands: monomer, dimer and trimer.")
![Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0005.jpg "Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0001.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0003.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0004.jpg)
![Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Western-Blot-DDX0470P-100-img0007.jpg "Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06")
![Immunohistochemistry-Frozen: TLR3 Antibody (619F7.06) [DDX0470P-100] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0470P-100. Staining of human tonsil section.](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Frozen-DDX0470P-100-img0003.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of canine blood cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (619F7.06) [DDX0470P-100] - Paraffin staining of Canine keratinocytes with TLR3 Antibody](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Paraffin-DDX0470P-100-img0001.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human breast carcinoma CAMA-1 cellline with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0004.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human monocyte-derived DCs and human EBV B cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0005.jpg)
![Western Blot: NALP6 Antibody [NBP2-31372] - WB detection of NALP6 protein (NACHT, LRR and PYD domains-containing protein 6) in (A) Jurkat cells lysate and (B) partial recombinant protein using NALP6 antibody at a concentration of 1 ug/ml for lysate and 0.1 ug/ml for the recombinant protein. In Jurkat cells lysate, this antibody detected a major band at ~98.8 kDa which the expected position for NALP6.](http://images.novusbio.com/fullsize/NALP6-Antibody-Western-Blot-NBP2-31372-img0001.jpg "Western Blot: NALP6 Antibody [NBP2-31372] - WB detection of NALP6 protein (NACHT, LRR and PYD domains-containing protein 6) in (A) Jurkat cells lysate and (B) partial recombinant protein using NALP6 antibody at a concentration of 1 ug/ml for lysate and 0.1 ug/ml for the recombinant protein. In Jurkat cells lysate, this antibody detected a major band at ~98.8 kDa which the expected position for NALP6.")
![Immunohistochemistry-Paraffin: NALP6 Antibody [NBP2-31372] - IHC-P analysis of NALP6 protein in a section of human breast normal tissue using NALP6 antibody at a concentration of 5 ug/ml. The breast ductal /acinar epithelium showed a strong NALP6 positivity in the cytoplasm and nuclei of the cells.](http://images.novusbio.com/fullsize/NALP6-Antibody-Immunohistochemistry-Paraffin-NBP2-31372-img0002.jpg)
![Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 2) Raji, 3) A431 whole cell lysates and 4) human tonsil tissue extract.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Western-Blot-NB110-87047-img0009.jpg "Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 2) Raji, 3) A431 whole cell lysates and 4) human tonsil tissue extract.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody (7F12H4) [NB110-87047] - The BTK antibody was tested in A431 cells against DyLight 488 (Green). Actin and nuclei were counterstained for Phalloidin (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Immunocytochemistry-Immunofluorescence-NB110-87047-img0010.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (7F12H4) [NB110-87047] - Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using BTK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Immunohistochemistry-Paraffin-NB110-87047-img0005.jpg)
![Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 1) truncated BTK recombinant protein and 2) K562 cell lysate.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Western-Blot-NB110-87047-img0006.jpg)
![Western Blot: Monkey IgG Isotype Control [NBP1-97047] - SDS-PAGE of Monkey IgG Whole Molecule. Lane 1: Non-Reduced Monkey IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker . Lane 3: Reduced Monkey IgG Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160 kDa, Observed at greater than 180; Reduced at 55, 25 kDa. Non-reduced sample migrates higher due to large molecular weight.](http://images.novusbio.com/fullsize/Monkey IgG Isotype Control-Western Blot-NBP1-97047-img0004.jpg "Western Blot: Monkey IgG Isotype Control [NBP1-97047] - SDS-PAGE of Monkey IgG Whole Molecule. Lane 1: Non-Reduced Monkey IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker . Lane 3: Reduced Monkey IgG Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160 kDa, Observed at greater than 180; Reduced at 55, 25 kDa. Non-reduced sample migrates higher due to large molecular weight.")
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0002.jpg "Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - The expression of TLR-7 in the hippocampal brain region. The immunofluorescence of TLR7 recognized by Alexa 488, green. GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license. TLR7 Antibody [NBP2-24906]")
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - An intracellular stain was performed on THP-1 cells with NBP2-24906 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. TLR7 Antibody [NBP2-24906]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0001.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Sections of nasal biopsies were incubated with antibodies against TLR9 (C) visualized by 3, 3-diaminobenzidine (brown). In control slides (F), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0098239) licensed under a CC-BY license. TLR7 Antibody [NB100-56682]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0003.jpg)
![Immunohistochemistry: TLR7 Antibody Pack [NBP2-31097] - Human brain using 1:50 dilution of TLR7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Punctate staining of neurons was observed. Staining was performed by Histowiz. TLR7 Antibody [NBP2-24767]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Immunohistochemistry-NBP2-31097-img0004.jpg)
![Flow Cytometry: TLR7 Antibody Pack [NBP2-31097] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary. TLR7 Antibody [NB100-56588]](http://images.novusbio.com/fullsize/TLR7-Antibody-Pack-Flow-Cytometry-NBP2-31097-img0005.jpg)
![Western Blot: IRAK4 Antibody (6F8) [DDX0340P-100] - Western Blot with 6F8 (Innateplateform, CLARA, Lyon)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Western-Blot-DDX0340P-100-img0011.jpg "Western Blot: IRAK4 Antibody (6F8) [DDX0340P-100] - Western Blot with 6F8 (Innateplateform, CLARA, Lyon)")
![Immunohistochemistry: IRAK4 Antibody (6F8) [DDX0340P-100] - IHC staining of human FFPE tonsil section(Vermi William courtesy)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Immunohistochemistry-DDX0340P-100-img0009.jpg)
![Flow Cytometry: IRAK4 Antibody (6F8) [DDX0340P-100] - Intracellular FACS Staining of canine cell line with 6F8 (Bonnefont C, personal comm, 2011).](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Flow-Cytometry-DDX0340P-100-img0003.jpg)
![Immunohistochemistry: IRAK4 Antibody (6F8) [DDX0340P-100] - Analysis of human tonsil with 6F8 (DDX0340)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Immunohistochemistry-DDX0340P-100-img0008.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0023.jpg "Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - 59T xenograft, using TRAF6 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung. TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0020.jpg)
![Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunoprecipitation-NBP1-33357-img0016.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-western-blot-26112025193619.jpg)
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.<br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:100.<br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-immunocytochemistry-immunofluorescence-2611202518503015.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0018.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33498-img0009.jpg)
![Immunohistochemistry-Paraffin: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Mouse cervix. PSME3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunohistochemistry-Paraffin-NBP1-33498-img0016.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0013.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: PC-12 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0014.jpg)
![Immunohistochemistry-Paraffin: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - CA922 xenograft, using PSME3 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunohistochemistry-Paraffin-NBP1-33498-img0010.jpg)
![Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP2-13159-img0002.jpg "Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-NBP2-13159-img0001.jpg)
![Simple Western: ZEB1 Antibody [NBP2-13159] - Lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Simple-Western-NBP2-13159-img0003.jpg)