| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33660-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-50056-20ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56457SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24397SS | |||
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Analysis in human esophagus and placenta tissues. Corresponding Chymase/CMA1/Mast Cell Chymase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Analysis in human esophagus and placenta tissues. Corresponding Chymase/CMA1/Mast Cell Chymase RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Aged control whole mount choroid immunolabeled for (A) UEA lectin (blue), (B) tryptase (red), and (C) chymase (green). The vast majority of human choroidal MCs are of the MCT phenotype (tryptase+/chymase-). Only a small percentage of MCs are MCtc (tryptase+/chymase+) (arrows). Scale bar: 30 um. Image collected and cropped by CiteAb from the following publication (//iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.17-22989), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33660-img0013.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0012.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human colon shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0009.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human esophagus shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0010.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human lymph node shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Aged control whole mount choroid immunolabeled for (A) UEA lectin (blue), (B) tryptase (red), & (C) chymase (green). The vast majority of human choroidal MCs are of the MCT phenotype (tryptase+/chymase−). Only a small percentage of MCs are MCtc (tryptase+/chymase+) (arrows). Scale bar: 30 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29164232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33660_rabbit-polyclonal-chymase-cma1-mast-cell-chymase-antibody-310202415291960.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Western-Blot-NBP1-51991-img0001.jpg "Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Flow-Cytometry-NBP1-51991-img0003.jpg)
![Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Immunofluorescence-NBP1-51991-img0002.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023922510.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023918332.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0005.jpg "Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry: MIF Antibody [NB100-1789] - Co-localization of CXCR4 and MIF in urothelium. Sections from rats treated with saline or CYP are shown. MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) and an overlay panel combining both immunostaining and a DAPI nuclear stain. MIF immunostaining is seen in basal and intermediate cells and in fibroblasts in the lamina propria of saline treated rats, while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal and intermediate cells of urothelium. CYP treatment resulted in superficial cell staining for MIF and CXCR4 and overlay panels demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar=50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0003898) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-NB100-1789-img0006.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Daudi (A) and Jurkat (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0003.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NB100-1789] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NB100-1789-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MIF Antibody [NB100-1789] - Co-localization of CXCR4 & MIF in urothelium.Representative sections from rats treated with saline (A–C) or CYP (D–I) are shown. The figure shows MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) & an overlay panel combining both immunostaining & a DAPI nuclear stain. MIF immunostaining is seen in basal & intermediate cells & in fibroblasts in the lamina propria of saline treated rats (A), while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal & intermediate cells of urothelium (B) & lamina propria is not stained. Overlay of these panels (C) demonstrate co-localization of MIF & CXCR4 as orange coloring of cells. CYP treatment resulted in superficial cell staining for MIF (D,G) & CXCR4 (E,H) & overlay panels (F,I) demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar = 50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19066630), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1789_goat-polyclonal-mif-antibody-31020241554531.jpg)
![Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Western-Blot-NBP2-37585-img0003.jpg "Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BLNK Antibody (5G9) [NBP2-37585] - Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Immunocytochemistry-Immunofluorescence-NBP2-37585-img0004.jpg)
![Immunohistochemistry-Paraffin: BLNK Antibody (5G9) [NBP2-37585] - Analysis of human cervical cancer tissues using BLNK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Immunohistochemistry-NBP2-37585-img0006.jpg)
![Flow Cytometry: BLNK Antibody (5G9) [NBP2-37585] - Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Flow-Cytometry-NBP2-37585-img0005.jpg)
![Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Western-Blot-NBP2-37585-img0002.jpg)
![ELISA: BLNK Antibody (5G9) [NBP2-37585] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-ELISA-NBP2-37585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD37 Antibody (2B8) [NBP1-28869] - Confocal immunofluorescence analysis of methanol-fixed BCBL-1 (left) and L1210 (right) cells using CD37 mouse mAb(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD37-Antibody-2B8-Immunocytochemistry-NBP1-28869-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD37 Antibody (2B8) [NBP1-28869] - Confocal immunofluorescence analysis of methanol-fixed BCBL-1 (left) and L1210 (right) cells using CD37 mouse mAb(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.")
![Immunohistochemistry-Paraffin: CD37 Antibody (2B8) [NBP1-28869] - Immunohistochemistry staining of CD37 (NBP1-28869: 2B8) on human paraffin-embedded spleen tissues, developed with NovaRed HRP substrate. Epitope retrieval: HIER Citrate. This image was submitted via customer review.](http://images.novusbio.com/fullsize/CD37-Antibody-2B8-Immunohistochemistry-Paraffin-NBP1-28869-img0004.jpg)
![Flow Cytometry: CD37 Antibody (2B8) [NBP1-28869] - CD37 FACS staining on BJAB cells (1:200), stained with GaM IgG1-Alexa488 secondary antibody. This image was submtted via customer review.](http://images.novusbio.com/fullsize/CD37-Antibody-2B8-Flow-Cytometry-NBP1-28869-img0005.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0014.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0002.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0003.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0005.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0006.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0008.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0009.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific OrangeTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0010.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) biotin.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0011.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of human peripheral blood with anti-CD16 (3G8) FITC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0012.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0013.jpg)
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0007.jpg "Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) purified, GAM-APC.")
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 antibody MEM-118.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0001.jpg)
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE-Cy(TM)5.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0002.jpg)
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0003.jpg)
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0004.jpg)
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0005.jpg)
![Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-MEM-118-Flow-Cell-Surface-NB500-395-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: CD4 Antibody [NB100-56457] - Analysis of CD4 in human thymus lysate using this antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/CD4-Antibody-Western-Blot-NB100-56457-img0002.jpg "Western Blot: CD4 Antibody [NB100-56457] - Analysis of CD4 in human thymus lysate using this antibody at 2 ug/ml.")
![Western Blot: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis using BLT1 antibody. Lysate from human muscle in the 1) absence, 2) presence of immunizing peptide probed with BLT1 antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/Leukotriene-B4-R1-Antibody-Western-Blot-NBP2-27422-img0004.jpg "Western Blot: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis using BLT1 antibody. Lysate from human muscle in the 1) absence, 2) presence of immunizing peptide probed with BLT1 antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis of human liver using BLT1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Leukotriene-B4-R1-Antibody-Immunohistochemistry-Paraffin-NBP2-27422-img0003.jpg)
![Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Western-Blot-NBP2-24461-img0004.jpg "Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Immunohistochemistry-Paraffin-NBP2-24461-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24397-img0002.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.")
![Western Blot: FoxP3 Antibody [NBP2-24397] - Analysis of FOXP3 in (A) FOXP3 transfected and (B) mock transfected cell lysate using NBP2-24397 at 5 ug/ml.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NBP2-24397-img0003.jpg)