| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-73845-10mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-73227 | |||
| FITC | NBP1-73177 | |||
| Rhodamine | NBP1-73175 | |||
| Texas Red | NBP1-73228 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB120-6015 | |||
| FITC | NB120-6009 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB120-6009 | |||
| Pre-adsorbed | NB120-6015 | |||
![Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Rat-IgM-Secondary-Antibody-[Rhodamine]-Western-Blot-NBP1-73379-img0004.jpg "Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73679] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Secondary-Antibody-[Biotin]-Western-Blot-NBP1-73679-img0004.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73679] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgM-Heavy-Chain-Secondary-Antibody-[HRP]-Dot-Blot-NBP1-73847-img0001.jpg "Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.")
![Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody [Rhodamine] [NBP1-73085] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Donkey-anti-Rat-IgG-H+L-Secondary-Antibody-Rhodamine-Western-Blot-NBP1-73085-img0004.jpg "Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody [Rhodamine] [NBP1-73085] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Western Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) [NB120-7139] - Western Blot of Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed).Lane 1: Molecular Weigh Ladder.Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Equine Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Lane 13: MW.Loading: 50ng IgG, Serum at 1:8 dilution.Blocking Buffer: Universal blocking buffer for 30 min at RT.Secondary Antibody: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) at 1:40000.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-HRP-Pre-adsorbed-Western-Blot-NB120-7139-img0004.jpg "Western Blot: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) [NB120-7139] - Western Blot of Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed).Lane 1: Molecular Weigh Ladder.Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Equine Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Lane 13: MW.Loading: 50ng IgG, Serum at 1:8 dilution.Blocking Buffer: Universal blocking buffer for 30 min at RT.Secondary Antibody: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) at 1:40000.")
![Western Blot of Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed). Lane 1: Molecular Weigh Ladder. Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Horse Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Lane 13: MW. Loading: 50ng IgG, Serum at 1:8 dilution. Blocking Buffer: BlockOut for 30 min at RT. Secondary Antibody: Gt-a-GP IgG HRP at 1:40,000.](http://images.novusbio.com/fullsize/nb120-7139_goat-polyclonal-goat-anti-guinea-pig-igg-h-l-secondary-antibody-hrp-pre-adsorbed-255202313137.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7594] - Dot Blot of Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed). Antigen: Mouse IgG. Load: 3-fold serial dilution starting at 200 ng.Primary antibody: None.Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) at 1:1000 for 60 min at RT.Block: 5% western blot blocking reagent for 1 Hour at RT.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-PE-Pre-adsorbed-Dot-Blot-NB7594-img0003.jpg "Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7594] - Dot Blot of Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed). Antigen: Mouse IgG. Load: 3-fold serial dilution starting at 200 ng.Primary antibody: None.Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) at 1:1000 for 60 min at RT.Block: 5% western blot blocking reagent for 1 Hour at RT.")
![Dot Blot: Goat anti-Human IgG Fc Secondary Antibody [NBP2-60677] - Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Gt a-Hu IgG F(c) FITC at 1 ug/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-Secondary-Antibody-Dot-Blot-NBP2-60677-img0001.jpg "Dot Blot: Goat anti-Human IgG Fc Secondary Antibody [NBP2-60677] - Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Gt a-Hu IgG F(c) FITC at 1 ug/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Image from the FITC version of this antibody.")
![ELISA: Goat anti-Human IgG Fc Secondary Antibody [NBP2-60677] - ELISA Results of Goat anti-Human IgG Fc Secondary antibody tested against purified HumanIgG Fc. Each well was coated in duplicate with 1.0 ug of HumanIgG Fc [Red Line], Human IgG Fab [Green Line], Human IgG [Blue Line]. The working dilution is 1:143,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Donkey Anti-Goat IgG HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgG-Fc-Secondary-Antibody-ELISA-NBP2-60677-img0004.jpg)
![ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72744] - ELISA Results of Rabbit anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) (Min X Human Serum Proteins) tested against purified Sheep IgG MX Hu. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Green Line] and Human IgG [Blue Line]. The working dilution of Sheep IgG MX Hu is 1:71,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72744-img0001.jpg "ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72744] - ELISA Results of Rabbit anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) (Min X Human Serum Proteins) tested against purified Sheep IgG MX Hu. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Green Line] and Human IgG [Blue Line]. The working dilution of Sheep IgG MX Hu is 1:71,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.")
![Western Blot: Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60682] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60682-img0002.jpg "Western Blot: Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60682] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None. Image using the FITC form of this antibody.")
![SDS-Page: Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60682] - SDS-PAGE results of Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: reduced F(ab')2 anti-Mouse.Lane 2: reduced F(ab')2 anti-Rabbit.Lane 3: Protein molecular weight standard.Lane 4: non-reduced F(ab')2 anti-Mouse.Lane 5: non-reduced F(ab')2 anti-Rabbit.Load: 1.0ug.4-20% Lonza SDS-PAGE; Coomassie Stained; visualized using western blot imaging system.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-SDS-Page-NBP2-60682-img0005.jpg)
![Western Blot: Rat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72765] - Western Blot of Rat anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Rat anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Rat IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72765-img0006.jpg "Western Blot: Rat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72765] - Western Blot of Rat anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Rat anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Rat IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.](http://images.novusbio.com/fullsize/Sheep-IgM-Isotype-Control-SDS-Page-NBP1-96774-img0001.jpg "SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.")
![Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [NB120-6838] - Western Blot of Chicken anti-Rat IgG (H+L) Secondary antibody. Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Chicken anti-Rat IgG (H+L) Secondary antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Chicken-anti-Rat-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6838-img0008.jpg "Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [NB120-6838] - Western Blot of Chicken anti-Rat IgG (H+L) Secondary antibody. Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Chicken anti-Rat IgG (H+L) Secondary antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Dot Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [NB120-6838] - 0.62 ng.Primary antibody: n/a.Secondary antibody: Chicken anti-Rat IgG (H+L) Secondary Antibody [FITC] at 1:1000 for 60 min at RT.Block: Fluorescent blocking buffer for 1 HR at RT.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Chicken-anti-Rat-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6838-img0007.jpg)