Chicken anti-Rat IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB120-6836] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
HRP

Order Details

Chicken anti-Rat IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Rat IgG whole molecule
Specificity
Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rat IgG and Rat Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • ELISA 1:10000 - 1:100000
  • Immunohistochemistry 1:500 - 1:2500
Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:12,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Reacts with rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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