Chicken anti-Rat IgG (H+L) Secondary Antibody [HRP]


Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB120-6836] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat more

Product Details

Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

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Chicken anti-Rat IgG (H L) Secondary Antibody [HRP] Summary

Rat IgG whole molecule
Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rat IgG and Rat Serum.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10mg/ml BSA
0.01% Gentamicin Sulfate
Immunogen affinity purified


  • Western Blot 1:1000 - 1:10000
  • ELISA 1:10000 - 1:100000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin
Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:12,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Reacts with rat.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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