Chicken anti-Rat IgG (H+L) Secondary Antibody [PE/Atto594]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Chicken
Conjugate
PE/Atto594

Chicken anti-Rat IgG (H+L) Secondary Antibody [PE/Atto594] Summary

Immunogen
This Chicken anti-Rat IgG (H+L) Secondary Antibody was developed against rat IgG whole molecule.
Specificity
Assay of Chicken anti-Rat IgG (H+L) Secondary Antibody by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken serum, Rat IgG and Rat serum.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

Alkaline Phosphatase NBP1-72629
Allophycocyanin/Cy7 NB120-6838APCCY7
Biotin NB120-6835
DyLight 405 NB120-6838V
DyLight 488 NB120-6838G
DyLight 550 NB120-6838R
DyLight 594 NB120-6838DL594
DyLight 650 NB120-6838C
DyLight 680 NB120-6838FR
DyLight 755 NB120-6838IR
FITC NBP1-73336
HRP NB120-6836
Janelia Fluor 549 NB120-6838JF549
Janelia Fluor 646 NB120-6838JF646
PE/Atto594 NB120-6838PEATT594
PE/Cy5.5 NB120-6838PECY55
PE/Cy7 NB120-6838PECY7
Rhodamine NB120-6833
Texas Red NB120-6834

Additional IgG (H+L) Products

Array NB120-6838PEATT594

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