Donkey anti-Rat IgG (H+L) Secondary Antibody [Biotin]


Western Blot: Donkey anti-Rat IgG (H+L) Secondary Antibody [Biotin] [NBP1-73148] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat more

Product Details

Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

Donkey anti-Rat IgG (H L) Secondary Antibody [Biotin] Summary

Rat IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rat IgG and Rat Serum.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:100,000 - 1:500,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reacts with Rat.


Restore with 1.0 mL of deionized water (or equivalent).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rat IgG, H/L Chains Antibody (NBP1-73148) (0)

There are no publications for Rat IgG, H/L Chains Antibody (NBP1-73148).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Rat IgG, H/L Chains Antibody (NBP1-73148) (0)

There are no reviews for Rat IgG, H/L Chains Antibody (NBP1-73148). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Rat IgG, H/L Chains Antibody (NBP1-73148) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Contact Information

Product PDFs

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-73148 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought