Chicken anti-Rat IgG (H+L) Secondary Antibody [Rhodamine]

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Western Blot: Chicken anti-Rat IgG (H+L) Secondary Antibody [Rhodamine] [NB120-6833] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Rhodamine
Concentration
LYOPH

Order Details

Chicken anti-Rat IgG (H+L) Secondary Antibody [Rhodamine] Summary

Immunogen
Rat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Rat IgG and Rat Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000

Reactivity Notes

Reacts with rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; Stabilizer: 10 mg/mL BSA - Immunoglobulin and Protease free.
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute to desired concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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