| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87760-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80826-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43790 | |||
| BSA Free | NBP2-46630-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47672-0.1mg | |||
| IHC-Prediluted | NBP2-48136 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56878SS | |||
![Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87760-img0021.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar protein distribution across tissues to independent antibody NBP1-87759 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Analysis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar pattern to independent antibody NBP1-87759 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87760-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87760-img0010.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0027.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0018.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0019.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0024.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0025.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0026.jpg)
![Simple Western: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Simple Western lane view shows a specific band for NFKB2 in 0.2 mg/ml of RT4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Simple-Western-NBP1-87760-img0006.jpg)
![Simple Western: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Electropherogram image(s) of corresponding Simple Western lane view. NFkB p100/p52 antibody was used at 1:30 dilution on RT4 lysate(s).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Simple-Western-NBP1-87760-img0007.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0015.jpg "Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: TRAF-6 Antibody [NBP1-80826] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80826-img0009.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0013.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0014.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0011.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0012.jpg)
![Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Western-Blot-NBP2-46630-img0008.jpg "Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunocytochemistry-Immunofluorescence-NBP2-46630-img0009.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0006.jpg)
![Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Western-Blot-NBP2-46630-img0007.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0001.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse cerebral cortex shows strong positivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0002.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0003.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse striatum shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0004.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebellum shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0005.jpg)
![Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Western-Blot-NBP2-67700-img0004.jpg "Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate")
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0005.jpg)
![Flow Cytometry: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of K562 cells with CCR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Flow-Cytometry-NBP2-67700-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0003.jpg)
![Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-VIPL2-BSA-Free-Flow-Cytometry-NBP1-97563-img0007.jpg "Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.")
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0001.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0002.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0003.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0004.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PerCP.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0005.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) FITC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32945-img0002.jpg "Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0010.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0004.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0008.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0009.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (NBP1-32945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32945_rabbit-polyclonal-syk-antibody-western-blot-261120251820234.jpg)
![Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:5000](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-3G10-Western-Blot-NB600-446-img0002.jpg "Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:5000")
![Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. Non-induced cell lysate from E. coli expressing GST-tagged proteinsB. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:1000](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-3G10-Western-Blot-NB600-446-img0001.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0006.jpg "Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).")
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0002.jpg)
![Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). conjugated with APC (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Flow-Cytometry-NBP2-45316-img0003.jpg)
![Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Western-Blot-NBP2-45316-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (LN-3 + HLA-DRB/1067).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunohistochemistry-Paraffin-NBP2-45316-img0001.jpg)
![Immunofluorescence: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with CF594 (Cyan).The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-LN-3-+-HLA-DRB-1067-Immunofluorescence-NBP2-45316-img0004.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0010.jpg "Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32192-img0002.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0012.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0005.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0011.jpg)
![ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-ELISA-NBP1-32192-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-141120251840435.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-1411202518443421.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0011.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.")
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the Biotin conjugate of NBP1-42772. Detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0006.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0007.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0010.jpg)
![Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56878-img0003.jpg "Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.")
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56878] - analysis of human pancreas using NOD1 antibody at 1:100.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56878-img0002.jpg)
![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0006.jpg "Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.")
![<b>Genetic Strategies Validation. </b>Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0007.jpg)
