| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22191-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33088 | |||
| IHC-Prediluted | NBP2-44988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47704-0.1mg | |||
| IHC-Prediluted | NBP2-48345 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56127SS | |||
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0006.jpg "Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Flow cytometric analysis of Hela cells using Integrin beta 1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Flow-Cytometry-NBP2-22191-img0001.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mAb against human ITGB1 (AA: 50-270) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0002.jpg)
![ELISA: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-ELISA-NBP2-22191-img0004.jpg)
![CyTOF-ready: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Analysis of Integrin beta 1/CD29 antibody on human tissue. Integrin beta 1/CD29 (green). Scale, 50um. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-CyTOF-ready-NBP2-22191-img0007.jpg)
![Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-VIPL2-BSA-Free-Flow-Cytometry-NBP1-97563-img0007.jpg "Flow Cytometry: Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free [NBP1-97563] - SUM159PT cells were stained with Integrin beta 3/CD61 antibody (NB100-64861AF488, 0.5 ug/10^6 cells). Left: unstain; Right: ITGB3 antibody stain. Image from verified customer review.")
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE-CyTM7.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0001.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0002.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC-CyTM7.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0003.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0004.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PerCP.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0005.jpg)
![Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) FITC.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-VIPL2-Flow-Cytometry-NBP1-97563-img0006.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (NBP1-30883). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0007.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:H129912% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0003.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0009.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0006.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-30883] - Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30883-img0005.jpg)
![Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFX1-Antibody-Western-Blot-NBP1-52654-img0006.jpg "Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: RFX1 Antibody [NBP1-52654] - Section stained with anti-RFX1 antibody. RFX1 belongs to Cluster 4. Spermatids were stained in brown. These distribution patterns were mostly consisted to that of trancripts. (Abbreviation: SPT=spermatid).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26753906/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-NBP1-52654-img0008.jpg)
![Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-Paraffin-NBP1-52654-img0004.jpg)
![Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunohistochemistry-Paraffin-NBP1-52654-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RFX1-Antibody-Immunoprecipitation-NBP1-52654-img0007.jpg)
![Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Western-Blot-NBP2-29465-img0007.jpg "Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunocytochemistry-Immunofluorescence-NBP2-29465-img0003.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0005.jpg)
![Flow Cytometry: CD74 Antibody (LN-2) [NBP2-29465] - Flow Cytometric Analysis of Human Raji cells using CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Flow-Cytometry-NBP2-29465-img0006.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0004.jpg)
![Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Western-Blot-NBP2-44471-img0017.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Western Blot Analysis of THP-1 and Raji cell lysates using beta 2-Microglobulin antibody (B2M/961).")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Immunofluorescence Analysis of HeLa cells labeling with beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunocytochemistry-Immunofluorescence-NBP2-44471-img0010.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0013.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Flow Cytometric Analysis of Human HeLa cells. beta 2-Microglobulin Antibody (B2M/961) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Flow-Cytometry-NBP2-44471-img0009.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Bladder Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0001.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0002.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0003.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Colon Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0004.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0005.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Melanoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0006.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Human Renal Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/961)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0007.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0011.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/961) [NBP2-44471] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (B2M/961).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-961-Immunohistochemistry-Paraffin-NBP2-44471-img0012.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0015.jpg "Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGECD10 antibody [N2C1], Internal dilution: 1:500](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0007.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0013.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127] - FFPE tissue section of human tonsil stained for CD40 expression using this antibody at 1:2000. Staining is seen in the dendritic cells. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-Immunohistochemistry-Paraffin-NB100-56127-img0003.jpg "Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127] - FFPE tissue section of human tonsil stained for CD40 expression using this antibody at 1:2000. Staining is seen in the dendritic cells. Hematoxylin-eosin counterstain.")
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32945-img0002.jpg "Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0010.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0004.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0008.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0009.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (NBP1-32945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32945_rabbit-polyclonal-syk-antibody-western-blot-261120251820234.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0023.jpg "Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - 59T xenograft, using TRAF6 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung. TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0020.jpg)
![Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunoprecipitation-NBP1-33357-img0016.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-western-blot-26112025193619.jpg)
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-immunocytochemistry-immunofluorescence-2611202518503015.jpg)