| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77299SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77855SS | |||
| Azide and BSA Free | NBP2-80773 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49045SS | |||
| Azide and BSA Free | NBP2-80658 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33122-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25195SS | |||
| Azide and BSA Free | NBP2-80659 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71615 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64021-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47670-0.1mg | |||
| IHC-Prediluted | NBP2-48112 | |||
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0014.jpg "Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 kDa.")
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Mouse Cell lines. 15 ug of lysates per lane. Antibodies: [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed molecular weight ~50 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0022.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis using RIPK3/RIP3 antibody [NBP1-77299]. L929 cell line (mouse). 20 ug whole cell lysate. Primary antibody: 1:2000. Secondary antibody: 1:20000. Western blot image submitted by a verified customer review. Observed molecular weight ~57 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0024.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - C2C12 Cells. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: NBP1-77299, 0.1 ug/mL in the presence of peptide blocking. Lane 2: NBP1-77299, 0.1 ug/mL Lane 3: NBP1-77299, 0.2 ug/mL Lane 4: NBP1-77299, 0.5 ug/mL. Observed molecular weight ~55 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0021.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP1-77299] - Bone-marrow-derived macrophages exhibit significant reduction in RIPK3 levels in Ripk3 delta Global, Ripk3 delta MΦ-Cre & Ripk3 delta EC-Cre mice. (A,B) BMDMs were isolated, differentiated on chamber slides for 7 days, immunostained for macrophage markers CD68 (red; A) & CD11b (pink; B), & co-stained for nuclei (Hoechst; blue). All cells from seven separate BMDM isolations were positive for these markers (n=7). (C) A phagocytosis assay was performed on BMDMs with fluorescent polystyrene beads. Phagocytic cells display TRITC+/Hoechst+ signal, & 85% of cells from two separate BMDM isolations were double positive (n=2). (D-F) Protein or RNA was collected from control, Ripk3 delta Global, Ripk3 delta MΦ-Cre & Ripk3 delta EC-Cre BMDMs. RNA was converted to cDNA & analyzed by qPCR for Ripk3 levels (D). Protein lysates were immunoblotted to identify CD11b, RIPK3 & beta -actin (loading control) (E) & quantified (F). (G) RNA from control & Ripk3 delta Global BMDMs was converted to cDNA & analyzed by qPCR for Ripk3-Exon 10 levels. For panels D,F,G, each dot represents a BMDM isolation from an individual animal. Statistics for panels D & F were calculated using one-way ANOVA with Dunnett's multiple comparisons test. Overall ANOVA P-values (prior to the post hoc tests) are 0.0002 (D) & 76%. Values are expressed as means +/- SEM; * p 76%. Values are expressed as means +/- SEM; * p < 0.05 versus WT group; #p < 0.05 versus WT-I/R group, (n = 6/group). (D) Graph showing increases in the levels of serum creatine in WT mice after renal I/R compared to sham-operated animals. However, in RIP3−/− mice, the level of serum creatine was reduced when compared to littermate WT mice after renal I/R. Note that there was no difference in the levels of serum creatine between WT and RIP3−/− sham mice. Values are expressed as means +/- SEM; * p < 0.05 versus WT-Sham group, respectively; #p < 0.05 versus WT-I/R group (n = 6/group). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35741025), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77299_rabbit-polyclonal-ripk3-rip3-antibody-knockout-validated-132202618215124.jpg)
![Dual RNAscope ISH-IHC: HLA-DR Antibody (L243) [NB100-77855] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for HLA-DR mRNA (ACD RNAScope probe, catalog # 475891; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NB100-77855) at 1:50 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Dual-RNAscope-ISH-IHC-NB100-77855-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: HLA-DR Antibody (L243) [NB100-77855] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for HLA-DR mRNA (ACD RNAScope probe, catalog # 475891; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NB100-77855) at 1:50 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HLA-DR Antibody (L243) [NB100-77855] - Analysis of human spleen (A) and human tonsil (B) tissue using HLA-DR antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Western-Blot-NB100-77855-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA-DR Antibody (L243) [NB100-77855] - Staining of HLA-DR in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Immunohistochemistry-Paraffin-NB100-77855-img0003.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the FITC conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with L243 FITC.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0015.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Human peripheral blood lymphocytes stained with L243 PE](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0001.jpg)
![Flow (Cell Surface): HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Azide Free version of NB100-77855. Staining of HLA-DR expression by 5x10^5 BDCM cells using 0.5 ug of (red) and 0.5 ug of isotype control (green). This antibody and an anti-mouse IgG PE conjugated secondary antibody were used.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cell-Surface-NB100-77855-img0010.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the PerCP/Cy5.5 conjugate of NB100-77855. Staining of HLA-DR expression by 5x10^5 human PBMC using 10 uL (0.25 ug) of this antibody. Cell surface flow kit was used for this test. Left panel shows the monocyte gate, right panel shows the lymphocyte gate.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0011.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77855. Staining of HLA-DR protein in human PBMCs using HLA-DR antibody [clone L243; conjugated to Alexa Fluor (R) 700] at 1:100 with 25 minutes incubation at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0012.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Biotin conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with biotinylated L243, followed by Sav-PE.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0013.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the DyLight 405 conjugate of NB100-77855. Staining of HLA-DR in human PBMCs using anti-HLA-DR antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0014.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0014.jpg "Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.")
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0011.jpg)
![Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/- , and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-NBP1-49045-img0016.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) - BSA Free [NBP1-49045] - Analysis of isolated mouse splenic CD8+ T cells using CD8 antibody (53-6.7) [Allophycocyanin] (NBP1-49045APC). Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-BSA-Free-Flow-Cytometry-NBP1-49045-img0017.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Frozen-NBP1-49045-img0008.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Immunohistochemistry-Frozen-NBP1-49045-img0015.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.](http://images.novusbio.com/fullsize/CD8-Antibody-53-6-7-Flow-Cytometry-NBP1-49045-img0009.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in human spleen tissue using anti-CD8 alpha antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-49045_rat-monoclonal-cd8-antibody-53-6-7-immunohistochemistry-paraffin-2210202583119.jpg)
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24588-img0004.jpg "Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Representative western blot analysis of RIP1, RIPK3/RIP3, and beta-actin in WI-38 NCs and IR-SCs 24 h after incubation with Veh or 10 uM PL. A cell lysate of etoposide-treated Jurkat cells was used as a positive control. Image collected and cropped by CiteAb from the following publication (//www.aging-us.com/article/101100/text) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody [NBP2-24588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with RIPK3/RIP3 Antibody [NBP2-24588] at a 1:200 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used at a 1:1000 dilution as a co-stain and detected with DyLight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24588-img0003.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 antibody [NBP2-24588]. Human tissue blot probed with 5 ug/ml of RIP3 antibody: 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, 9) spleen, 10) ovary, 11) testis, 12) placenta probed at 5 ug/ml, 13) Ramos cell lysate probed at 7 ug/ml. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0005.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP2-24588] - HOIP limits the activity of TRAIL‐induced apoptosis‐ & necroptosis‐mediating signalling complexesControl & HOIP KO HeLa cells were treated with FLAG‐lz‐TRAIL (500 ng/ml) for the indicated times. The TRAIL complex I was immunoprecipitated via anti‐FLAG beads. Complex II was isolated by immunoprecipitating caspase‐8 from complex I‐depleted lysates. Western blot was performed using the indicated antibodies. * indicates unspecific bands.WT & HOIP‐deficient TNF KO MEFs, pre‐treated for 1 h with zVAD, were stimulated with iz‐TRAIL (1 μg/ml) for the indicated times. FADD‐containing complexes were immunoprecipitated & analysed by Western blot.Data information: See also Fig EV2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28258062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24588_rabbit-polyclonal-ripk3-rip3-antibody-31020241534394.jpg)
![Western Blot: RIPK3/RIP3 Antibody - BSA Free [NBP2-24588] - The catalytic activity of HOIP contributes to preventing TRAIL‐induced apoptosis but is dispensable for preventing necroptosisLysates from control or HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S analysed by WB.HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD and/or Nec‐1s for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 5; mean ± SEM). *P < 0.05, **P < 0.01; statistics performed using ANOVA.Control & HOIP KO K562 cells reconstituted w/ empty vector, HOIP WT or HOIP C885S treated w/ iz‐TRAIL (100 ng/ml) for the indicated times. Lysates analysed w/ the indicated antibodies.Lysates from TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S analysed by WB.TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD & Nec‐1s for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 4; mean ± SEM). *P < 0.05, ***P < 0.001; statistics performed using ANOVA.TNF KO HOIP KO MEFs reconstituted w/ empty vector, pre‐treated w/ zVAD and/or the RIPK3 inhibitor GSK'872 for 1 h as indicated, treated w/ iz‐TRAIL (1 μg/ml) for 24 h. Percentage of cell death determined by flow cytometry after PI labelling (n = 3; mean ± SEM). *P < 0.05; statistics performed using t‐test.TNF KO HOIP KO MEFs reconstituted w/ empty vector, HOIP WT or HOIP C885S, pre‐treated w/ zVAD for 1 h, treated w/ iz‐TRAIL (1 μg/ml) for the indicated times. FADD‐containing complexes immunoprecipitated & analysed by WB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28258062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24588_rabbit-polyclonal-ripk3-rip3-antibody-310202416114733.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with rat anti-mouse CD19, clone 6D5 green in A and rat anti-mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Immunohistochemistry-Frozen-NB200-578-img0006.jpg "Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with rat anti-mouse CD19, clone 6D5 green in A and rat anti-mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power")
![Flow Cytometry: CD8 Antibody (YTS105.18) [NB200-578] - Staining of mouse spleen cells.](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Flow-Cytometry-NB200-578-img0003.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of Mouse lymph node cryosection with CD8 Antibody and HRP-conjugated goat anti-rat IgG. Low power magnification.](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Immunohistochemistry-Frozen-NB200-578-img0005.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-Immunohistochemistry-Frozen-NBP2-29475-img0002.jpg "Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. aCcdc88b mRNA expression in distal colons of NT (n = 3) or DSS-treated WT mice (n = 3 for each time point) at the indicated days. Data represent expression relative to hprt±SEM (n = 3). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test) & are representative of one experiment. b Representative immunoblots for Ccdc88b protein detected in extracts from distal colons from NT or DSS-treated WT & Ccdc88Bmut mutant mice at indicated time points, & densitometric quantification of Ccdc88b immunoblot normalized to beta -actin±SEM (n = 3) (representative of one of two independent experiments); **P < 0.01 (two-tailed Student’s t-test). c Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at indicated times, & stained with antibodies against Ccdc88b (red), CD45 (green), & E-Cadherin (purple) & nuclei staining DAPI (blue). Scale bars, 100 μm. d Representative FACS plots & quantification of lamina propria cells stained for CD45 & for Ccdc88b antibodies for NT (n = 3) & DSS-treated WT mice (n = 4 for each time point) at indicated time points, data are representative of one experiment. e Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, & stained for Ccdc88b (red), CD3 (green) & CD11b (green); nuclei are stained with DAPI (blue). Scale bars, 100 μm. f same as in e for Ccdc88b (red), CD4 (green) & CD8 (green). Scale bars, 100 μm Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01381-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29475_rabbit-polyclonal-cd8-antibody-imgenex-abx-160a-20ug-310202416113534.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0015.jpg "Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMC using CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0012.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the DyLight 405 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMCs using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0013.jpg)
![Flow (Cell Surface): CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of Human peripheral blood mononuclear cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cell-Surface-NBP2-25195-img0014.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of CD8 in 10^6 human PBMC using 10 ul (0.1 ug) of this antibody. The shaded histogram represents human PBMC alone; green represents mouse isotype control, red represents human CD8 this antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-1772024104463.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the FITC conjugate of NBP2-25195. Staining of CD8 in human PBMCs using 0.25 ug of (clone RPA-T8) per approximately 1x10^6 cells. Green represents isotype control this antibody, red represents anti-CD8 FITC antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-17720241044620.jpg)
![Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Western-Blot-NB100-2817-img0002.jpg "Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).")
![Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Immunohistochemistry-Paraffin-NB100-2817-img0004.jpg)
![Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Small Intestine. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AGO1-EIF2C1-Antibody-Immunohistochemistry-Paraffin-NB100-2817-img0003.jpg)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0010.jpg "Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunocytochemistry-Immunofluorescence-NBP2-02627-img0009.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0008.jpg)
![Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0002.jpg)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0003.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0004.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0005.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0006.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0007.jpg)
![Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0001.jpg)
![Immunohistochemistry: CD8 Antibody (12.C7) [NB100-64021] - scAAV8G9-optHLA-G Combo Prevents Cornea Burn-induced Vascularization and Cytotoxic T-cell Infiltration. Rabbit cornea sections were acquired 60 days following the injection of indicated vectors into burn corneas and stained for an endothelial cell marker (CD31), T cell markers, transgene abundance, and a-SMA in the indicated treatment groups. Scale bars=5um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-18002-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-NB100-64021-img0002.jpg "Immunohistochemistry: CD8 Antibody (12.C7) [NB100-64021] - scAAV8G9-optHLA-G Combo Prevents Cornea Burn-induced Vascularization and Cytotoxic T-cell Infiltration. Rabbit cornea sections were acquired 60 days following the injection of indicated vectors into burn corneas and stained for an endothelial cell marker (CD31), T cell markers, transgene abundance, and a-SMA in the indicated treatment groups. Scale bars=5um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-18002-9), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD8 Antibody (12.C7) [NB100-64021] - Human bile duct cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-Paraffin-NB100-64021-img0003.jpg)
![Flow Cytometry: CD8 alpha Antibody (12.C7) [NB100-64021] - Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 Alpha followed by Goat anti Mouse IgG (H/L):FITC](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-12-C7-Flow-Cytometry-NB100-64021-img0001.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Western-Blot-NBP2-71615-img0002.jpg "Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.")
![Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71615-img0005.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0009.jpg)
![Flow Cytometry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71615-img0004.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Western-Blot-NBP2-71615-img0001.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0006.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0007.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0008.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0010.jpg)
![Flow Cytometry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71615-img0003.jpg)
![Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Western Blot Analysis of Ramos cell lysate using HLA-DR antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Western-Blot-NBP2-45310-img0014.jpg "Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Western Blot Analysis of Ramos cell lysate using HLA-DR antibody (LN-3).")
![Immunocytochemistry/Immunofluorescence: HLA-DR Antibody (LN-3) [NBP2-45310] - Immunofluorescence staining of Ramos cells using HLA-DRMouse Monoclonal Antibody (LN-3) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Immunocytochemistry-Immunofluorescence-NBP2-45310-img0011.jpg)
![Immunohistochemistry-Paraffin: HLA-DR Antibody (LN-3) [NBP2-45310] - Human Histiocytoma stained with HLA-DRB Monoclonal Antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Immunohistochemistry-Paraffin-NBP2-45310-img0004.jpg)
![Flow Cytometry: HLA-DR Antibody (LN-3) [NBP2-45310] - Flow Cytometric Analysis of Raji cells. HLA-DR Mouse Monoclonal Antibody (LN-3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Flow-Cytometry-NBP2-45310-img0012.jpg)
![Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Flow Cytometry: HLA-DR Antibody (LN-3) - Azide and BSA Free [NBP2-34676] - Analysis using the PE conjugate of NBP2-34676. Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PE (blue histogram) or Anti-Human HLA-DR PE (purple histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Western-Blot-NBP2-45310-img0007.jpg)
![Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Western Blot Analysis of Ramos cell and human spleen lysate using HLA-DR Mouse Monoclonal Antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Western-Blot-NBP2-45310-img0013.jpg)
![Immunohistochemistry-Paraffin: HLA-DR Antibody (LN-3) [NBP2-45310] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Immunohistochemistry-Paraffin-NBP2-45310-img0003.jpg)
![Flow Cytometry: HLA-DR Antibody (LN-3) [NBP2-45310] - Flow Cytometry: HLA-DR Antibody (LN-3) - Azide and BSA Free [NBP2-34676] - Analysis using the PE/Cy7 conjugate of NBP2-34676. Staining of HLA-DR in human buffy coat using anti-HLA-DR antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Flow-Cytometry-NBP2-45310-img0010.jpg)