| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6405SS | |||
| Azide and BSA Free | NBP2-80848 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44302SS | |||
| Azide and BSA Free | NBP2-44304SS | |||
| Azide and BSA Free | NBP2-44304 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44300SS | |||
| Azide and BSA Free | NBP2-44301SS | |||
| Azide and BSA Free | NBP2-44301 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85978-25ul | |||
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P TAPII > MHCII, which increased after IL-17 treatment. (A) MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P ≤0.01). (B) MHCII revealed a fainter basic expression emphasizing the nucleus and was found significantly increased after 0.5 ng/mL IL-17 stimulation (**P ≤ 0.01). (C) TAPII was detected in the nucleus and cytoplasm. We detected a dose-dependent tendency but no significantly increased expression after IL-17 stimulation. For MHCI, TAPII and MHCII and the concentrations applied, analysis of variance between the independent experiments revealed no significant difference. DAPI, 4′, 6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24678820), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85960_rabbit-polyclonal-sfxn2-antibody-western-blot-13220261739815.jpg "Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P TAPII > MHCII, which increased after IL-17 treatment. (A) MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P ≤0.01). (B) MHCII revealed a fainter basic expression emphasizing the nucleus and was found significantly increased after 0.5 ng/mL IL-17 stimulation (**P ≤ 0.01). (C) TAPII was detected in the nucleus and cytoplasm. We detected a dose-dependent tendency but no significantly increased expression after IL-17 stimulation. For MHCI, TAPII and MHCII and the concentrations applied, analysis of variance between the independent experiments revealed no significant difference. DAPI, 4′, 6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24678820), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-33661-img0011.jpg "Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-33661] - Analysis of methanol-fixed HeLa, using Syntenin 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33661-img0002.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0006.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Rat fore brain. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0007.jpg)
![Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse brain.Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-immunohistochemistry-frozen-261120251818924.jpg)
![Zebrafish tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-western-blot-261120251836599.jpg)
![Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse brain.Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-immunohistochemistry-frozen-26112025182708.jpg)
![Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RAB35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20042-img0011.jpg "Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - Human breast cancer, using RAB35 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB35-Antibody-Immunohistochemistry-Paraffin-NBP2-20042-img0002.jpg)
![Western Blot: RAB35 Antibody [NBP2-20042] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB35-Antibody-Western-Blot-NBP2-20042-img0009.jpg)
![Western Blot: RAB35 Antibody [NBP2-20042] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB35-Antibody-Western-Blot-NBP2-20042-img0010.jpg)
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.RAB35 stained by RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-91020242011500.jpg)
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.RAB35 stained by RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-9102024205618.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-western-blot-26112025199275.jpg)
![Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-western-blot-261120251837504.jpg)
![Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-Azide-Free-Flow-Cytometry-NBP2-21789-img0001.jpg "Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.")
![Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-western-blot-362024112212..jpg "Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112318..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112412..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112523..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112620..jpg)
![Flow Cytometry: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of HL-60 cells labeling MHC class I.Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-flow-cytometry-362024112742..jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue labeling MHC class I with Rabbit anti-MHC class I antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 c, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunocytochemistry-immunofluorescence-362024112932..jpg)
![Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serum](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Western-Blot-NBP2-67511-img0009.jpg "Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serum")
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0004.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0003.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded human liver cancer tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0005.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0006.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0007.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0008.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0010.jpg)
![Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Western-Blot-NBP2-44302-img0005.jpg "Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)")
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (6B12NB) [NBP2-44302] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (6B12NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Immunocytochemistry-Immunofluorescence-NBP2-44302-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) [NBP2-44302] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 6B12NB). The antibody generated a punctate staining in the cytoplasm/perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Immunohistochemistry-Paraffin-NBP2-44302-img0003.jpg)
![Flow (Cell Surface): HLA DMA Antibody (6B12NB) [NBP2-44302] - Analysis using the Azide Free version of NBP2-44302. Detection of Human PBMC cells (surface) with HLA DMA (6B12NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Flow-Cell-Surface-NBP2-44302-img0008.jpg)
![Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Western-Blot-NBP2-44300-img0004.jpg "Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.")
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (5C9NB) [NBP2-44300] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (5C9NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Immunocytochemistry-Immunofluorescence-NBP2-44300-img0005.jpg)
![Immunohistochemistry: HLA DMA Antibody (5C9NB) [NBP2-44300] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 5C9NB). The antibody generated a diffused to punctate staining in cellular membranes and cytoplasm, especially in the perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Immunohistochemistry-NBP2-44300-img0003.jpg)
![Flow (Cell Surface): HLA DMA Antibody (5C9NB) [NBP2-44300] - Analysis using the Azide Free version of NBP2-44300. Detection of Human PBMC cells (surface) with HLA DMA (5C9NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Flow-Cell-Surface-NBP2-44300-img0007.jpg)
![Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Western-Blot-NBP2-76852-img0001.jpg "Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Immunocytochemistry-Immunofluorescence-NBP2-76852-img0003.jpg)
![Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Immunohistochemistry-NBP2-76852-img0006.jpg)
![Flow Cytometry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Flow cytometric analysis of LOVO cells with Nectin 2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Flow-Cytometry-NBP2-76852-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Immunocytochemistry-Immunofluorescence-NBP2-76852-img0002.jpg)
![Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Immunohistochemistry-NBP2-76852-img0004.jpg)
![Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Nectin-2-CD112-Antibody-JG39-64-Immunohistochemistry-NBP2-76852-img0005.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Western-Blot-NBP2-20866-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vitronectin stained by Vitronectin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0008.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Human colon carcinoma, using Vitronectin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0003.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (NBP2-20866) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20866_rabbit-polyclonal-vitronectin-antibody-910202420104489.jpg)
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-86979] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86979-img0005.jpg "Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-86979] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol.")
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-86979] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86979-img0014.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-86979] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86979-img0009.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-86979] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86979-img0012.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-86979] - Staining of human placenta shows weak tcytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86979-img0013.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0013.jpg "Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Stain ing of human tonsil shows low positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0006.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0007.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0008.jpg)
![Immunohistochemistry-Paraffin: KIF1C Antibody [NBP1-85978] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KIF1C-Antibody-Immunohistochemistry-Paraffin-NBP1-85978-img0009.jpg)
