| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-235SS | |||
| Azide and BSA Free | NBP2-80597 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84357-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77030-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84356-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94471-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92608-0.02ml | |||
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0028.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunohistochemistry-Paraffin-NB600-235-img0025.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-BSA-Free-Flow-Cytometry-NB600-235-img0032.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0027.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0011.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0013.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0015.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0024.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0029.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0030.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - The effect of IL-21 & CD40L exposure on MEC1 & MEC2 cells.Expression of EBNA-2 & LMP-1 in IL-21 treated cells (A, B). (A) Simultaneous immunofluorescence staining of EBNA-2 (Green) & LMP-1 (Red); magnification (×100), scale bar 25 µm. Note the downregulation of EBNA-2 & upregultion of LMP-1 after IL-21 treatment. (B) Expression of EBNA-2, LMP-1 & Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in the control lanes & 5×105 were loaded in both untreated & IL-21 treated MEC1 & MEC2 lanes. Note low expression of EBNA-2 & high expression of LMP-1 after IL-21 treatment & induction of Blimp-1 after IL-21 treatment. (C) Activity of the W & C promoters that regulate EBNA-2 expression & LMP-1 mRNA expression by Q-PCR. Note the difference in EBNA-2 regulation; the MEC2 cell uses both Wp & Cp while in MEC1 only Wp is active. (D) Expression of EBNA-2 & LMP-1 in cells exposed to CD40L. Simultaneous immunofluorescence staining; for details see (A). Note: EBNA-2 & LMP-1 are downregulated by CD40L in both lines. (E) CD40L induced modulation of surface marker by FACS analysis. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-235_mouse-monoclonal-blimp1-prdm1-antibody-3h2-e8-31020241616096.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human testis using Anti-TEX101 antibody NBP1-84357.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0014.jpg "Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human testis using Anti-TEX101 antibody NBP1-84357.")
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0006.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining in human testis and kidney tissues using anti-TEX101 antibody. Corresponding TEX101 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human cerebral cortex, colon, testis and tonsil using Anti-TEX101 antibody NBP1-84357 (A) shows similar protein distribution across tissues to independent antibody NBP1-84356 (B).](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0010.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human tonsil.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0011.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0012.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84357] - Staining of human colon.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84357-img0013.jpg)
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0012.jpg "Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0006.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP1-77030-img0011.jpg)
![Immunohistochemistry: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunohistochemistry Validation of SCF/c-kit Ligand in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCF/c-kit Ligand antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-2711202420441113.jpg)
![Immunocytochemistry/ Immunofluorescence: SCF/c-kit Ligand Antibody - BSA Free [NBP1-77030] - Immunofluorescence Validation of SCF/c-kit Ligand in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF/c-kit Ligand with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77030_rabbit-polyclonal-scf-c-kit-ligand-antibody-271120241928812.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Western-Blot-NBP1-51991-img0001.jpg "Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Flow-Cytometry-NBP1-51991-img0003.jpg)
![Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Immunofluorescence-NBP1-51991-img0002.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023922510.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023918332.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human testis using Anti-TEX101 antibody NBP1-84356.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0011.jpg "Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human testis using Anti-TEX101 antibody NBP1-84356.")
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining in human testis and endometrium tissues using anti-TEX101 antibody. Corresponding TEX101 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human cerebral cortex, colon, testis and tonsil using Anti-TEX101 antibody NBP1-84356 (A) shows similar protein distribution across tissues to independent antibody NBP1-84357 (B).](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0007.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human tonsil.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0008.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0009.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP1-84356] - Staining of human colon.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP1-84356-img0010.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180] - Analysis of BLIMP-1 using BLIMP-1 polyclonal antibody in cattle skin extracts from 5 different animals. Protein expression of increased 1.5-4 fold when measured 24 h after tick infection. (loading was standardized with a GAPDH antibody). BLIMP-1 was detected at ~88 kDa. Data courtesty of Kongsuwan et al.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Western-Blot-NBP2-27180-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180] - Analysis of BLIMP-1 using BLIMP-1 polyclonal antibody in cattle skin extracts from 5 different animals. Protein expression of increased 1.5-4 fold when measured 24 h after tick infection. (loading was standardized with a GAPDH antibody). BLIMP-1 was detected at ~88 kDa. Data courtesty of Kongsuwan et al.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0005.jpg "Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0002.jpg)
![Immunoprecipitation: Alpha Actinin 3 Antibody [NBP2-15356] - Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 ug of alpha Actinin 3 antibody. Western blot analysis was performed using alpha Actinin 3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunoprecipitation-NBP2-15356-img0004.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15356 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15356_rabbit-polyclonal-alpha-actinin-3-antibody-9102024205628.jpg)
![Western Blot: TEX101 Antibody [NBP2-94471] - Western blot analysis of extracts of A-549 cells, using TEX101 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/TEX101-Antibody-Western-Blot-NBP2-94471-img0001.jpg "Western Blot: TEX101 Antibody [NBP2-94471] - Western blot analysis of extracts of A-549 cells, using TEX101 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP2-94471] - Immunohistochemistry of paraffin-embedded Mouse testis using TEX101 Rabbit pAb (NBP2-94471) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP2-94471-img0004.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP2-94471] - Immunohistochemistry of paraffin-embedded Human colon using TEX101 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP2-94471-img0002.jpg)
![Immunohistochemistry-Paraffin: TEX101 Antibody [NBP2-94471] - Immunohistochemistry of paraffin-embedded Rat testis using TEX101 Rabbit pAb (NBP2-94471) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TEX101-Antibody-Immunohistochemistry-Paraffin-NBP2-94471-img0003.jpg)
![Western Blot: SCF/c-kit Ligand Antibody [NBP2-27162] - Analysis of SCF in mouse liver lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for test.](http://images.novusbio.com/fullsize/SCF-c-kit-Ligand-Antibody-Western-Blot-NBP2-27162-img0002.jpg "Western Blot: SCF/c-kit Ligand Antibody [NBP2-27162] - Analysis of SCF in mouse liver lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for test.")
![Western Blot: Alpha Actinin 3 Antibody (4H7E6) [NBP3-15827] - Western blot analysis of extracts of various cell lines, using Alpha Actinin 3 Rabbit mAb (NBP3-15827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-4H7E6-Western-Blot-NBP3-15827-img0003.jpg "Western Blot: Alpha Actinin 3 Antibody (4H7E6) [NBP3-15827] - Western blot analysis of extracts of various cell lines, using Alpha Actinin 3 Rabbit mAb (NBP3-15827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: Alpha Actinin 3 Antibody (4H7E6) [NBP3-15827] - Immunofluorescence analysis of mouse bone marrow cells using Alpha Actinin 3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15827_rabbit-alpha-actinin-3-mab-4h7e6-7220251245395.jpg)
![Immunocytochemistry/ Immunofluorescence: Alpha Actinin 3 Antibody (4H7E6) [NBP3-15827] - Immunofluorescence analysis of rat bone marrow cells using Alpha Actinin 3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15827_rabbit-alpha-actinin-3-mab-4h7e6-7220251245318.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-92608] - Analysis of extracts of various cell lines, using Alpha Actinin 3 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-92608-img0002.jpg "Western Blot: Alpha Actinin 3 Antibody [NBP2-92608] - Analysis of extracts of various cell lines, using Alpha Actinin 3 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Alpha Actinin 3 Antibody [NBP2-92608] - Immunohistochemistry of paraffin-embedded rat skeletal muscle using Alpha Actinin 3 Rabbit pAb (NBP2-92608) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-92608-img0004.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 3 Antibody [NBP2-92608] - Immunohistochemistry of paraffin-embedded mouse skeletal muscle using Alpha Actinin 3 Rabbit pAb (NBP2-92608) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-92608-img0003.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody [NBP3-17184] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Western-Blot-NBP3-17184-img0002.jpg "Western Blot: BLIMP1/PRDM1 Antibody [NBP3-17184] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP3-17184] - Staining of human tonsil shows cytoplasmic positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Immunohistochemistry-Paraffin-NBP3-17184-img0001.jpg)
