| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36561SS | |||
| Azide and BSA Free | NBP2-80818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22191-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84576-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44404 | |||
| BSA Free | NBP2-76520-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44402 | |||
| BSA Free | NBP2-76518-25ul | |||
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0005.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Overnight incubation with 1:50 Integrin beta 1/CD29 primary antibody in PBS/BSA 1%, at 4ºC. Green: Integrin beta 1/CD29 Antibody (P4C10), Red: Phalloidin, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-36561_mouse-monoclonal-integrin-beta-1-cd29-antibody-p4c10-immunocytochemistry-immunofluorescence-1462023134549..jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Integrin-beta-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16974-img0003.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-16974] - Human colon carcinoma, using Integrin beta 1 / CD29 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15minof paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Integrin-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16974-img0002.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1/CD29 antibody (NBP2-16974) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16974_rabbit-polyclonal-integrin-beta-1-cd29-antibody-91020242011496.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16974_rabbit-polyclonal-integrin-beta-1-cd29-antibody-1610202419414566.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Western-Blot-NB500-383-img0003.jpg "Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0006.jpg "Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Flow cytometric analysis of Hela cells using Integrin beta 1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Flow-Cytometry-NBP2-22191-img0001.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mAb against human ITGB1 (AA: 50-270) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0002.jpg)
![ELISA: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-ELISA-NBP2-22191-img0004.jpg)
![CyTOF-ready: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Analysis of Integrin beta 1/CD29 antibody on human tissue. Integrin beta 1/CD29 (green). Scale, 50um. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-CyTOF-ready-NBP2-22191-img0007.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0004.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of A-549 cells, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0002.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0003.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and K-562 cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517303123.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517352792.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734518.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Human placenta using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734594.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363728.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517344298.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Rat large intestine using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733528.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363712.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-87MG cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733588.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517333885.jpg)
![Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serum](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Western-Blot-NBP2-67511-img0009.jpg "Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serum")
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0004.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0003.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded human liver cancer tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0005.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0006.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0007.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0008.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-ST49-02-Immunohistochemistry-Paraffin-NBP2-67511-img0010.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Western-Blot-NBP2-67883-img0009.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0007.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Flow-Cytometry-NBP2-67883-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human endometrium shows weak to moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0014.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0010.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0011.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0013.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Western-Blot-NBP2-20866-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vitronectin stained by Vitronectin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0008.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Human colon carcinoma, using Vitronectin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0003.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (NBP2-20866) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20866_rabbit-polyclonal-vitronectin-antibody-910202420104489.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Western-Blot-NBP2-76520-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0005.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Western-Blot-NBP2-76520-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human placenta and skeletal muscle tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human placenta shows strong positivity in apical membranes of trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human liver shows strong positivity in liver sinusoids.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human prostate shows strong membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0007.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Western-Blot-NBP2-76518-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0005.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Western-Blot-NBP2-76518-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in human placenta and testis tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human endometrium shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0004.jpg)