| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76614-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56542SS | |||
| Azide and BSA Free | NBP2-33224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58345SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80035-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80038-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33220 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76611-0.025mg | |||
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 10 ug/ml for 1 hour at room temperature followed by incubation with Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0009.jpg "Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 10 ug/ml for 1 hour at room temperature followed by incubation with Goat anti-Mouse IgG Secondary Antibody.")
![Western Blot: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of ACE2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human kidney tissue lysateLane 2: human small intestine tissue lysate](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Western-Blot-NBP2-67692-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunocytochemistry-Immunofluorescence-NBP2-67692-img0003.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Rabbit Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 3 ug/ml overnight at 4 degrees C. Detection kit: VC003. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0010.jpg)
![Western Blot: ACE-2 Antibody (SN0754) [NBP2-67692] - Western blot analysis of ACE-2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE-2 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Western-Blot-NBP2-67692-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunocytochemistry-Immunofluorescence-NBP2-67692-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunocytochemistry-Immunofluorescence-NBP2-67692-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ACE2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0004.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded mouse kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-SN0754-Immunohistochemistry-Paraffin-NBP2-67692-img0006.jpg)
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0010.jpg "In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.")
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0012.jpg)
![IKK gamma Inhibitor Peptide Set [NBP2-26504] - The IKK complex consists of IKKalpha + IKKbeta+ NEMO. NEMO (IKKgamma/NF-kB Essential MOdulator) is a scaffold protein required for the kinase activity of IKKalpha + IKKbeta. NBD (NEMO Binding Domain) inhibits formation of the IKK complex (IKKalpha + IKKbeta+ NEMO) by binding to NEMO and blocking IKK complex formation. In this regard, NBD is considered to be an 'IKKalpha and IKKBeta decoy' Blocking IKK complex formation suppresses downstream events in the NF-kB signaling pathway that rely on the IKK complex including phosphorylation of IkBalpha, and subsequence IkBalpha ubiquination, degradation and release of NF-kB. Hence, NF-kB is retained in the cytoplasm in its inactive state.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-NBP2-26504-img0014.jpg)
![Functional (Inhibition): IKK gamma Inhibitor Peptide Set [NBP2-26504] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-26504) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-26504-img0016.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76614] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), ACE2, (2 ug/mL), ACE2, (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76614-img0009.jpg "Western Blot: ACE-2 Antibody [NBP1-76614] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), ACE2, (2 ug/mL), ACE2, (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-76614] - Analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-76614-img0005.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76614] - Validation in Rat Skin Tissue Loading: 15 ug of lysates per lane.Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76614-img0006.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76614] - Loading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76614-img0007.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76614] - Validation in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76614-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody [NBP1-76614] - Analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 at 20 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76614-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ACE-2 Antibody [NBP1-76614] - Analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 at 20 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76614-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76614] - Validation of ACE-2 in Rat Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76614_rabbit-polyclonal-ace-2-antibody-2711202420175165.gif)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76614] - Validation of ACE-2 in Human Testis Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76614_rabbit-polyclonal-ace-2-antibody-2711202419514040.gif)
![Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Western-Blot-NB100-56542-img0005.jpg "Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.")
![Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Simple-Western-NB100-56542-img0004.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0009.jpg "Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.")
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)
![Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Western-Blot-NBP2-80035-img0003.jpg "Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).")
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) [NBP2-80035] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80035) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Immunohistochemistry-Paraffin-NBP2-80035-img0006.jpg)
![Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - Detection of endogenous human ACE2 by anti-ACE2 (h), mAb (AC18F). HepG2 cell line treated with CellTase solution (Abeomics) before analysis and then stained with anti-ACE2 (h), mAb (AC18F) (red line) or an isotype control, mouse IgG1 (green line) at 1ug/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Flow-Cytometry-NBP2-80035-img0005.jpg)
![Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Flow-Cytometry-NBP2-80035-img0001.jpg)
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0003.jpg "Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).")
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 0.5 ug/mL sheep Fc-tagged recombinant human Spike S1 is shown to bind ACE-2 (black dotted line) detected by Donkey anti-Sheep APC Secondary Antibody (Catalog # F0127). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0004.jpg)
![Western Blot: ACE-2 Antibody (AC384) [NBP2-80038] - 1: Human ACE2 Fc-fusion protein. 2: Human ACE2 (Ecto domain) (FLAG®-tagged). 3: HepG2 cell lysate. 4: Other Fc-fusion (hGITR-Fc) proteins (negative control). 1:2'000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Western-Blot-NBP2-80038-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC384) Monoclonal Antibody (Catalog # NBP2-80038) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0007.jpg)
![Flow Cytometry: ACE-2 Antibody (AC384) [NBP2-80038] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # NBP2-80038) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Flow-Cytometry-NBP2-80038-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 was detected in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-HumanACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80038) at 15ug/mL for 1 hour at room temperature followed by incubation with the Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0006.jpg)
![Functional: ACE-2 Antibody (AC384) [NBP2-80038] - Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384). Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) is coated on an ELISA plate at 1ug/ml. ACE2 (human), mAb (blocking) (AC384) or an unrelated mAb Control are added (starting at 40 ug/mL with a two-fold serial dilution) together with 500 ng/uL of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RAB35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20042-img0011.jpg "Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - Human breast cancer, using RAB35 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB35-Antibody-Immunohistochemistry-Paraffin-NBP2-20042-img0002.jpg)
![Western Blot: RAB35 Antibody [NBP2-20042] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB35-Antibody-Western-Blot-NBP2-20042-img0009.jpg)
![Western Blot: RAB35 Antibody [NBP2-20042] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB35-Antibody-Western-Blot-NBP2-20042-img0010.jpg)
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.RAB35 stained by RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-91020242011500.jpg)
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.RAB35 stained by RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-9102024205618.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-western-blot-26112025199275.jpg)
![Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-western-blot-261120251837504.jpg)
![Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Western-Blot-NB100-56532-img0007.jpg "Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IKK gamma Antibody (46B844) [NB100-56532] - Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone and an anti-mouse IgG-FITC secondary antibody.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Flow-Cytometry-NB100-56532-img0005.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76611] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 ug of lysates per lane. Antibodies: ACE2, NBP1-76614 (2 ug/mL), ACE2, NBP1-76612 (2 ug/mL), ACE2, NBP1-76611 (2 ug/mL) and beta-actin NBP1-76692 (1 ug/ml).](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76611-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ACE-2 Antibody [NBP1-76611] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 ug of lysates per lane. Antibodies: ACE2, NBP1-76614 (2 ug/mL), ACE2, NBP1-76612 (2 ug/mL), ACE2, NBP1-76611 (2 ug/mL) and beta-actin NBP1-76692 (1 ug/ml).")
![<b>Independent Antibodies Validation. </b>Western Blot: ACE-2 Antibody [NBP1-76611] - Loading: 15 ug of lysates per lane. Antibodies: ACE2, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76611-img0005.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76611] - Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76611-img0006.jpg)
![Western Blot: ACE-2 Antibody [NBP1-76611] - Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Western-Blot-NBP1-76611-img0007.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-76611] - Human kidney cells using Angiotensin Converting Enzyme 2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-76611-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Human Testis Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76611_rabbit-polyclonal-ace-2-antibody-271120241932692.gif)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 In Caco2 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.](http://images.novusbio.com/fullsize/nbp1-76611_rabbit-polyclonal-ace-2-antibody-2711202420441179.gif)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Human Lung Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76611_rabbit-polyclonal-ace-2-antibody-2711202419241397.gif)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Rat Lung Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76611_rabbit-polyclonal-ace-2-antibody-2711202419592045.gif)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Mouse Lung Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76611_rabbit-polyclonal-ace-2-antibody-2711202419553090.gif)
![Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Human Kidney Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE-2 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76611_rabbit-polyclonal-ace-2-antibody-2711202420243346.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining in human small intestine and tonsil tissues.. Corresponding ACE2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88123-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining in human small intestine and tonsil tissues.. Corresponding ACE2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88123-img0014.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human duodenum shows strong positivity in apical membranes in glandular cells.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88123-img0011.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human kidney shows strong positivity in apical membrane in cells in tubules.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88123-img0012.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88123-img0013.jpg)
![Flow Cytometry: ACE-2 Antibody [NBP1-88123] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human ACE-2 Polyclonal Antibody (Catalog # NBP1-88123) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins.](http://images.novusbio.com/fullsize/ACE-2-Antibody-Flow-Cytometry-NBP1-88123-img0016.jpg)
![Western Blot: MKKS Antibody [NBP1-92123] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MKKS-Antibody-Western-Blot-NBP1-92123-img0006.jpg "Western Blot: MKKS Antibody [NBP1-92123] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: MKKS Antibody [NBP1-92123] - Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92123-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0012.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92123 (A) shows similar protein distribution across tissues to independent antibody NBP1-92122 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0009.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0010.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0011.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92123] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92123-img0013.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92123_rabbit-polyclonal-mkks-antibody-295202516101016.jpg)