| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-318-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88700-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22540-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88701-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90585-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48214 | |||
| Azide and BSA Free | NBP3-11490 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50431-0.025ml | |||
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Western-Blot-NBP2-22541-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of frozen rat spleen with Mouse anti Rat CD54.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Frozen-NBP2-22541-img0011.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0014.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse kidney tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Paraffin-NBP2-22541-img0001.jpg)
![Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-NBP2-22541-img0002.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse spleen tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Paraffin-NBP2-22541-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of stimulated rat spleen cells with mouse anti-rat CD54 (Alexa488).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0010.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Ramos cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0012.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Raji cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0013.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normalized gene & protein expression of ICAM-1. (A) qPCR results indicated no change in mRNA expression of ICAM-1 at 24 h post-overpressure but a significant increase at 48 h as compared to sham. (B) Subsequent Western blot results confirmed elevated ICAM-1 protein levels at 48 h compared to sham. *p < 0.05, Data are represented as mean ± SEM, gene: n = 7–9/group, protein: n = 8–9/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30853931), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22541_mouse-monoclonal-icam-1-cd54-antibody-1a29-31020241536370.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated, compared to single exposure, radiation more profoundly enhanced ICAM-1 expression. Representative (3 independent experiments) Western blot analysis & quantification of ICAM-1 levels in whole-cell lysates from nonirradiated (sham) & irradiated HUVECs 4 h & 24 h after exposure to (a) either five fractions of 2 Gy (5 × 2 Gy) or single exposure to 10 Gy & (b) either five fractions of 2.5 Gy (5 × 2.5 Gy) or single exposure to 12.5 Gy. Fractions delivered at 24-h intervals. beta -actin served as a loading control. c Ectopic expression of ICAM-1 after exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy, as measured by flow cytometry (n = 3). d NF-kappa B activation after 4 h of exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy (n = 2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22541_mouse-monoclonal-icam-1-cd54-antibody-1a29-31020241533490.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated thoracic irradiation suppressed KLF2 & enhanced ICAM-1 levels in the lung. Quantification of KLF2 (a) & ICAM-1 (b) protein levels & representative Western blot analysis (c) in the lung tissue of mice (n = 6) at 24 h following 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. beta -actin served as a loading control. KLF2 immunostaining in the lung tissue samples of sham irradiated (d), irradiated (e), & quantitation (f) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. ICAM-1 immunostaining in the lung tissue samples of sham irradiated (g), or irradiated (h), & quantitation (i) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. (n, number of independent experiments performed; a, significant statistical difference between nonirradiated & irradiated groups; b, significant statistical difference between fractionated irradiation & single exposure; *p < 0.05; **p < 0.01; ***p < 0.001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22541_mouse-monoclonal-icam-1-cd54-antibody-1a29-31020241611356.jpg)
![Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of paclitaxel compared with normal lung tissue; p < 0.01. These changes were reversed by using parecoxib in combination with paclitaxel, while the group that received parecoxib treatment alone resembled the Con group. The bar graph under the protein strap represents the corresponding band intensities; the data are presented as x +/- s and corrected by GA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12977), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Western-Blot-NB500-318-img0006.jpg "Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of paclitaxel compared with normal lung tissue; p < 0.01. These changes were reversed by using parecoxib in combination with paclitaxel, while the group that received parecoxib treatment alone resembled the Con group. The bar graph under the protein strap represents the corresponding band intensities; the data are presented as x +/- s and corrected by GA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12977), licensed under a CC-BY license.")
![Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Flow-Cytometry-NB500-318-img0005.jpg)
![Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Western-Blot-NB500-318-img0001.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using the FITC conjugate of NB500-318. Staining of Human peripheral blood lymphocytes with MEM-111 FITC.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Flow-Cytometry-NB500-318-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using PE conjugate of NB500-318. A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-MEM-111-Flow-Cytometry-NB500-318-img0004.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-ICAM1 antibody NBP1-88700 (A) shows similar protein distribution across tissues to independent antibody NBP1-88701 (B).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0009.jpg)
![Western Blot: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Western-Blot-NBP1-88700-img0005.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0014.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lymph node shows moderate membranous positivity.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0011.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0012.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0013.jpg)
![Western Blot: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of 25 ug of NIH-3T3 (lane 1), mouse spleen (lane 2) and Ramos (lane 3) cell lysates.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-3E2B-Western-Blot-NBP2-22540-img0006.jpg "Western Blot: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of 25 ug of NIH-3T3 (lane 1), mouse spleen (lane 2) and Ramos (lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of CD54 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-3E2B-Immunocytochemistry-Immunofluorescence-NBP2-22540-img0005.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse spleen tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-3E2B-Immunohistochemistry-Paraffin-NBP2-22540-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of CD54 in mouse splenocytes (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-3E2B-Flow-Cytometry-NBP2-22540-img0007.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse kidney tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-3E2B-Immunohistochemistry-Paraffin-NBP2-22540-img0001.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-3E2B-Immunohistochemistry-Paraffin-NBP2-22540-img0002.jpg)
![Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cell-Surface-NB110-58732-img0010.jpg "Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.")
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0004.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Fig. 1. Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0005.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Staining of human peripheral blood with anti-CD54 (1H4) APC.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0007.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Staining of human peripheral blood with anti-CD54 (1H4) Alexa Fluor (R) 700.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0009.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0006.jpg)
![Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of ICAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: HUVEC cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Western-Blot-NBP2-67518-img0007.jpg "Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of ICAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: HUVEC cell lysate")
![Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Immunocytochemistry-Immunofluorescence-NBP2-67518-img0002.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Immunohistochemistry-Paraffin-NBP2-67518-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Immunocytochemistry-Immunofluorescence-NBP2-67518-img0001.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human kidney tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Immunohistochemistry-Paraffin-NBP2-67518-img0003.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human lung tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Immunohistochemistry-Paraffin-NBP2-67518-img0004.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human spleen tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-ST0487-Immunohistochemistry-Paraffin-NBP2-67518-img0005.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining in human lung and skeletal muscle tissues.. Corresponding ICAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88701-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining in human lung and skeletal muscle tissues.. Corresponding ICAM1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-ICAM1 antibody NBP1-88701 (A) shows similar protein distribution across tissues to independent antibody NBP1-88700 (B).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88701-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ICAM-1/CD54 Antibody [NBP1-88701] - Analysis in human cell lines SK-MEL-30 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Western-Blot-NBP1-88701-img0013.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining of human lung shows membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88701-img0017.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88701-img0009.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining of human skeletal muscle shows no positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88701-img0011.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88701] - Staining of human lymph node shows moderate membranous positivity.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88701-img0016.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0005.jpg "Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Flow-Cytometry-NBP2-66896-img0004.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: Raji cell lysateLane 2: Daudi cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0001.jpg)
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue)](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0002.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-009-Flow-Cytometry-NBP2-90585-img0001.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Immunofluorescence staining of Mouse ICAM-1/CD54 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90585 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90585_rabbit-monoclonal-icam-1-cd54-antibody-009-23520252135170.png)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0002.jpg "Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.")
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse brain with rabbit monoclonal antibody (1:200). The image showing positive staining of choroid plexus.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0001.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)) [NBP2-44331] - Human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-W-CAM-1-same-as-Wehi-CAM-1-or-1H4-Immunohistochemistry-Paraffin-NBP2-44331-img0001.jpg "Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)) [NBP2-44331] - Human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).")