GFP Antibody


Western Blot: GFP Antibody [NB100-56402] - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody (NB100-56402) at 1:1000 (0.5ug/ml).
Western Blot: GFP Antibody [NB100-56402] - Analysis of Green Fluorescent Protein in 20 ugs of A) transfected HeLa cells and B) untransfected HeLa cells using Green Fluorescent Protein antibody at 0.5 ug/ml.

Product Details

Reactivity NASpecies Glossary
Applications WB
1.0 mg/ml

Order Details

GFP Antibody Summary

Amino acids 204-217 of Green Fluorescent Protein were used as immunogen.
The Green Fluorescent Protein amino acid sequence used as immunogen for this antibody is 100% homologous in YFP (Yellow) and CFP (Cyan).
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 0.5-2 ug/ml
Read Publications using
NB100-56402 in the following applications:

  • WB
    1 publication

Reactivity Notes


Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein


Green Fluorescent Protein (GFP) is often used as a reporter molecule for gene expression. Cotransfecting Green Fluorescent Protein with a gene of interest allows researchers to rapidly determine the success of a transfection.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for GFP Antibody (NB100-56402)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFP Antibody (NB100-56402). (Showing 1 - of FAQs).

Secondary Antibodies


Isotype Controls

Additional GFP Antibody Products

Research Areas for GFP Antibody (NB100-56402)

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Blogs on GFP.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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