His Tag Antibody (3D5) [DyLight 755]


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Product Details

Reactivity Hu, All-NASpecies Glossary
Applications WB, ELISA
DyLight 755

His Tag Antibody (3D5) [DyLight 755] Summary

Additional Information
Recombinant Monoclonal Antibody.
Purified his tagged Fab fragment.
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
IgG Kappa
Protein A purified
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  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for His Tag Antibody (3D5) [DyLight 755]

  • 6 His epitope tag
  • 6-His Tag
  • 6X His Tag
  • 6X His
  • 6x-His Tag
  • 6X-His
  • H
  • Hexa His tag
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • His6-Tag
  • polyhistidine tag
  • polyHistidine
  • Poly-histidine


A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).


1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52637AF350
Alexa Fluor 405 NBP2-52637AF405
Alexa Fluor 488 NBP2-52637AF488
Alexa Fluor 532 NBP2-52637AF532
Alexa Fluor 594 NBP2-52637AF594
Alexa Fluor 647 NBP2-52637AF647
Alexa Fluor 700 NBP2-52637AF700
Alexa Fluor 750 NBP2-52637AF750
Biotin NBP2-52637B
DyLight 350 NBP2-52637UV
DyLight 405 NBP2-52637V
DyLight 488 NBP2-52637G
DyLight 550 NBP2-52637R
DyLight 594 NBP2-52637DL594
DyLight 650 NBP2-52637C
DyLight 680 NBP2-52637FR
DyLight 755 NBP2-52637IR
FITC NBP2-52637F
HRP NBP2-52637H

Additional His Tag Products

Array NBP2-52637IR

Research Areas for His Tag Antibody (NBP2-52637IR)

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Contact Information

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Product PDFs


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