HA Tag Antibody (16.43)


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Product Details

Reactivity ETSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
1 mg/ml

Order Details

HA Tag Antibody (16.43) Summary

This HA Tag Antibody (16.43) was developed against the C-terminal influenza virus hemagglutinin (HA) tag, YPYDVPDYA.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
This HA Tag Antibody (16.43) antibody is Cytof ready.
Read Publications using NBP2-50416.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Protein G purified


Positive control(s): Any protein with a C-terminal HA tag..

Alternate Names for HA Tag Antibody (16.43)

  • HA Epitope Tag
  • HA Tag
  • HA
  • Hemagglutinin


Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).


1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (NBP2-50416)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HA Tag Antibody (NBP2-50416). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
    • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50416AF350
Alexa Fluor 405 NBP2-50416AF405
Alexa Fluor 488 NBP2-50416AF488
Alexa Fluor 532 NBP2-50416AF532
Alexa Fluor 594 NBP2-50416AF594
Alexa Fluor 647 NBP2-50416AF647
Alexa Fluor 700 NBP2-50416AF700
Alexa Fluor 750 NBP2-50416AF750
Allophycocyanin NBP2-50416APC
Allophycocyanin/Cy7 NBP2-50416APCCY7
Biotin NBP2-50416B
DyLight 350 NBP2-50416UV
DyLight 405 NBP2-50416V
DyLight 488 NBP2-50416G
DyLight 550 NBP2-50416R
DyLight 594 NBP2-50416DL594
DyLight 650 NBP2-50416C
DyLight 680 NBP2-50416FR
DyLight 755 NBP2-50416IR
FITC NBP2-50416F
HRP NBP2-50416H
Janelia Fluor 549 NBP2-50416JF549
Janelia Fluor 646 NBP2-50416JF646
PE NBP2-50416PE
PE/Atto594 NBP2-50416PEATT594
PE/Cy5.5 NBP2-50416PECY55
PE/Cy7 NBP2-50416PECY7
PerCP NBP2-50416PCP

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Blogs on HA Tag.

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