E-Tag Epitope Tag Antibody [HRP]

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Western Blot: E-Tag Epitope Tag Antibody [HRP] [NB600-526] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).

Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC (-)
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

E-Tag Epitope Tag Antibody [HRP] Summary

Immunogen
Goats were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Western Blot 1:1000-1:10000
Application Notes
This antibody is useful for ELISA, Western Blot and Immunocytochemistry. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated.
Readout System
Publications
Read Publication using NB600-526.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for E-Tag Epitope Tag Antibody [HRP]

  • E epitope tag
  • GAPVPYPDPLEPR epitope tag
  • GAPVPYPDPLEPR tag

Background

E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Tag Epitope Tag Antibody (NB600-526)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for E-Tag Epitope Tag Antibody (NB600-526) (0)

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Secondary Antibodies

 

Isotype Controls

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