Goat anti-Mouse IgG F(ab)2 Secondary Antibody [Unconjugated] (Pre-adsorbed)

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Western Blot: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588] - Western Blot: Goat anti-Mouse IgG Secondary Antibody [Rhodamine] [NB120-6786] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588]
Immunohistochemistry: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588] - Analysis of paraffin embedded human colonic tissue using Goat anti-Mouse IgG [Cy5] secondary antibody. Image from verified ...read more
Flow Cytometry: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588]
Immunohistochemistry: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.
Dot Blot: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat ...read more
Dot Blot: Goat anti-Mouse IgG F(ab)2 Secondary Antibody (Pre-absorbed) [NB7588] - Dot Blot: Goat anti-Mouse IgG Secondary Antibody [Rhodamine] [NB120-6786] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
Pre-adsorbed
Concentration
2 mg/ml

Order Details

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Goat anti-Mouse IgG F(ab)2 Secondary Antibody [Unconjugated] (Pre-adsorbed) Summary

Immunogen
Mouse IgG F(ab')2 fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(ab)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1
Publications
Read Publication using NB7588.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB7588)(1)

We have publications tested in 2 applications: IHC-Fr, IHC-FrFl.


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(1)
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(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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