![Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Western-Blot-NBP2-59941-img0007.jpg "Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa")
| Reactivity | BaSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | SS 3A5-E2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | GST fused to A. aeolicus BPL/BioID2 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Positive Control: Cells overexpressing A. aeolicus BPL R40G (BioID2) construct. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-59941 | Applications | Species |
|---|---|---|
| Choi, J, Phelan, J D Et al. Regulation of B cell receptor-dependent NF- kappa B signaling by the tumor suppressor KLHL14. Proc Natl Acad Sci U S A 2020-03-17 [PMID: 32127472] (WB) | WB |
Secondary Antibodies |
Isotype Controls |
Research Areas for A. aeolicus BPL/BioID2 Antibody (NBP2-59941)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0005.jpg "Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.")
![Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0002.jpg "Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")