| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37471-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52517-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB92171-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56348SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92685SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26296SS | |||
| Azide and BSA Free | NBP2-80716 | |||
![Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-Western-Blot-NBP2-37537-img0003.jpg "Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: MSH6 Antibody (3E1) [NBP2-37537] - Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP2-37537-img0004.jpg)
![Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of colon cancer tissues using MSH6 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-Immunohistochemistry-NBP2-37537-img0007.jpg)
![Flow Cytometry: MSH6 Antibody (3E1) [NBP2-37537] - Flow cytometric analysis of MCF-7 cells using MSH6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-Flow-Cytometry-NBP2-37537-img0005.jpg)
![Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mAb against human MSH6 (AA: 217-395) recombinant protein. (Expected MW is 45.5 kDa)](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-Western-Blot-NBP2-37537-img0002.jpg)
![Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of rectum cancer tissues using MSH6 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-Immunohistochemistry-NBP2-37537-img0006.jpg)
![ELISA: MSH6 Antibody (3E1) [NBP2-37537] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MSH6-Antibody-3E1-ELISA-NBP2-37537-img0001.jpg)
![Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Western-Blot-NBP2-22178-img0005.jpg "Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of U251 cells using NFkB p105/p50 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Immunocytochemistry-Immunofluorescence-NBP2-22178-img0002.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFkB p105/p50 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Immunohistochemistry-Paraffin-NBP2-22178-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Flow cytometric analysis of MCF-7 cells using NFkB p105/p50 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Flow-Cytometry-NBP2-22178-img0001.jpg)
![Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of A431 (A) and HeLa (B) cell lysate using NFkB p105/p50 (NBP2-22178) antibody at 1:500.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-Western-Blot-NBP2-22178-img0008.jpg)
![ELISA: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-5D10D11-ELISA-NBP2-22178-img0004.jpg)
![Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-Western-Blot-NBP2-37471-img0003.jpg "Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: INCENP Antibody (3D2) [NBP2-37471] - Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-Immunocytochemistry-Immunofluorescence-NBP2-37471-img0004.jpg)
![Immunohistochemistry-Paraffin: INCENP Antibody (3D2) [NBP2-37471] - Analysis of rectum cancer tissues using INCENP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-Immunohistochemistry-NBP2-37471-img0007.jpg)
![Flow Cytometry: INCENP Antibody (3D2) [NBP2-37471] - Analysis of Jurkat cells using INCENP mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-Flow-Cytometry-NBP2-37471-img0005.jpg)
![Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa)](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-Western-Blot-NBP2-37471-img0002.jpg)
![Immunohistochemistry-Paraffin: INCENP Antibody (3D2) [NBP2-37471] - Analysis of cervical cancer tissues using INCENP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-Immunohistochemistry-NBP2-37471-img0006.jpg)
![ELISA: INCENP Antibody (3D2) [NBP2-37471] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/INCENP-Antibody-3D2-ELISA-NBP2-37471-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Western-Blot-NBP2-52517-img0006.jpg "Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of MCF-7 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunofluorescence-NBP2-52517-img0003.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of paraffin-embedded brain tissues using NFKBIA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunohistochemistry-Paraffin-NBP2-52517-img0005.jpg)
![Flow Cytometry: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Flow-Cytometry-NBP2-52517-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Western-Blot-NBP2-52517-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunofluorescence-NBP2-52517-img0002.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of paraffin-embedded liver cancer tissues using NFKBIA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-Immunohistochemistry-Paraffin-NBP2-52517-img0004.jpg)
![ELISA: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4C4-ELISA-NBP2-52517-img0010.jpg)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western blot analysis on different lysates with NBP2-66856 at 1/5,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66856 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66856_-western-blot-639204586669447398.jpg)
![Western blot analysis on different lysates with NBP2-66856 at 1/5,000 dilution. Lane 1: C2C12 cell lysate Lane 2: C2C12 treatment with 300 nM thapsigargin for 18h cell lysate Lane 3: C6 cell lysate Lane 4: C6 treatment with 300 nM thapsigargin for 18h cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-66856, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 19 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/nbp2-66856_-western-blot-639204598086981387.jpg)
![Western Blot: MCM7 Antibody (2068C) [MAB92171] - Analysis of HeLa, MCF-7, and MOLT-4, Daudi cell lysates with 0.5 ug/ml of hMCM7 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hMCM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/MCM7-Antibody-2068C-Western-Blot-MAB92171-img0002.jpg "Western Blot: MCM7 Antibody (2068C) [MAB92171] - Analysis of HeLa, MCF-7, and MOLT-4, Daudi cell lysates with 0.5 ug/ml of hMCM7 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hMCM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: MCM7 Antibody (2068C) [MAB92171] - MCM-7 was detected in immersion fixed HeLa cell using MCM-7 Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/MCM7-Antibody-2068C-Immunocytochemistry-MAB92171-img0001.jpg)
![Immunohistochemistry: MCM7 Antibody (2068C) [MAB92171] - MCM-7 was detected in immersion fixed paraffin-embedded sections of human mesothelioma using MCM-7 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.](http://images.novusbio.com/fullsize/MCM7-Antibody-2068C-Immunohistochemistry-MAB92171-img0003.jpg)
![Flow Cytometry: MCM7 Antibody (2068C) - Azide Free [MAB92171] - HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB92171, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/MCM7-Antibody-2068C-Azide-Free-Flow-Cytometry-MAB92171-img0004.jpg)
![Western Blot: Histone H4 Antibody [NB100-56348] - Analysis of Histone H4 in human PBMC cell lysate with NB100-56348.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NB100-56348-img0004.jpg "Western Blot: Histone H4 Antibody [NB100-56348] - Analysis of Histone H4 in human PBMC cell lysate with NB100-56348.")
![Western Blot: BRCAA1 Antibody [NBP1-26618] - Whole cell lysate from HeLa cells. ARID4B was also immunoprecipitated by rabbit anti-ARID4B antibody NBP1-26619.](http://images.novusbio.com/fullsize/BRCAA1-Antibody-Western-Blot-NBP1-26618-img0007.jpg "Western Blot: BRCAA1 Antibody [NBP1-26618] - Whole cell lysate from HeLa cells. ARID4B was also immunoprecipitated by rabbit anti-ARID4B antibody NBP1-26619.")
![Immunohistochemistry: BRCAA1 Antibody [NBP1-26618] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID4B used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/BRCAA1-Antibody-Immunohistochemistry-NBP1-26618-img0010.jpg)
![Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.](http://images.novusbio.com/fullsize/TAF15-Antibody-4D71-Western-Blot-NBP1-92685-img0002.jpg "Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.")
![Immunocytochemistry/Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, NBP1-92685, dilution 1:500 in red, and costained with rabbit pAb to GAPDH, dilution 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The NBP1-92685 antibody reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.](http://images.novusbio.com/fullsize/TAF15-Antibody-4D71-Immunocytochemistry-Immunofluorescence-NBP1-92685-img0001.jpg)
![Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/XPA-Antibody-Western-Blot-NB100-93321-img0009.jpg "Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.](http://images.novusbio.com/fullsize/XPA-Antibody-Immunohistochemistry-NB100-93321-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: XPA Antibody [NB100-93321] - Detection of human XPA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPA antibody NB100-93321 (lot NB100-93321-3) used for IP at 6 ug per reaction. XPA was also immunoprecipitated by a previous lot of this antibody (lot NB100-93321-2) and another rabbit anti-XPA antibody. For blotting immunoprecipitated XPA, NB100-93321 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/XPA-Antibody-Immunoprecipitation-NB100-93321-img0007.jpg)
![Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0021.jpg "Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-547-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.](http://images.novusbio.com/fullsize/PLK1-Antibody-Western-Blot-NB100-547-img0020.jpg)
![Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Western-Blot-NBP2-26296-img0003.jpg "Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Immunocytochemistry-Immunofluorescence-NBP2-26296-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using eIF2A (3A7B11) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and perinuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Immunohistochemistry-Paraffin-NBP2-26296-img0005.jpg)