| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-42761 | |||
| HRP | NBP1-42762 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-1131SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44513 | |||
| Azide and BSA Free | NBP2-34569-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70454 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71027 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97494-0.2mg | |||
| BSA Free | NBP1-97494-0.025mg | |||
![Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NB600-1131-img0014.jpg "Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.")
![Immunohistochemistry: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains and tumors were stained with anti-CD31, anti-IL-6, and anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal and tumor tissues. Bar represents 50?um. Zoom-in factor: 4 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03050-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1131-img0015.jpg)
![Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NB600-1131-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. a Human brain ECs were treated with glioma-CM for 24 h, & cell lysates were subjected to multiplex cytokine array analysis. Left, a representative blot. Right, quantified dot intensity of most significantly changed cytokines. b Human microvascular brain ECs were treated with glioma-CM that were harvested from different human glioma cells. Cell lysates were immunoblotted. c Human microvascular brain ECs & tumor-associated ECs isolated from different GBM patients were subjected to immunoblot analysis. d Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains & tumors were stained with anti-CD31, anti-IL-6, & anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal & tumor tissues. Bar represents 50 μm. Zoom-in factor: 4 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29422647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1131_rabbit-polyclonal-il-6-antibody-310202416154648.jpg)
![Western Blot: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Knockout-Validated-NBP2-25208-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution and proportion of CD86+ macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (d). The results were expressed as Mean +/- SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD and OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS and ##; P < 0.01 vs either NS or OVA/BUD group. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-020-1322-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE-/- mice. Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green), M1 marker CD86 (red) and DAPI (blue) (n = 5, respectively). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fphar.2018.00464/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0010.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0008.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0007.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution & proportion of CD68+ (a), CD86+ (c) & CD163+ (e) macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (b, d, f). The results were expressed as Mean ± SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD & OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS & ##; P < 0.01 vs either NS or OVA/BUD group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32111211), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-imgenex-img-6882a-25ug-31020241534324.jpg)
![Immunocytochemistry/ Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE–/– mice. (A,B) Co-immunofluorescence staining of the aortic root for macrophage (anti-Moma-2 antibody, green) & M1 marker iNos (red), & a bar graph summarizing the results (n = 5, respectively). (C,D) Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green) & M1 marker CD86 (red), & a bar graph summarizing the results (n = 5, respectively). Scale bar: 20 μm. Data are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29867472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-imgenex-img-6882a-25ug-31020241535650.jpg)
![M1 macrophages were induced in the in vivo mouse intervertebral disc (mIVD) degeneration model (puncture model). (a) Immunohistological analysis of Iba-1, iNOS, and arginase (brown staining) expression in nucleus pulposus (NP), annulus fibrosis (AF), and cartilage endplate (CEP) [right] at high magnification and in whole mIVDs (left) at low magnification. Images using an IgG isotype control antibody are included to demonstrate the specificity of the IHC staining. (b) Protein was extracted from punctured mIVDs in the puncture model, and western blotting was performed using antibodies against CD86, CD163, and beta -actin. (c) Images of the panel in (b), captured using a ChemiDoc Touch system and quantified using ImageJ. Biological and technical replicates were performed three times. Values represent the mean +/- SD. *p < 0.05, **p < 0.01, relative to the corresponding control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/41053148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-western-blot-13220261327102.jpg)
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Western-Blot-NBP1-47708-img0008.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Crystallin AB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0011.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0013.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Flow-Cytometry-NBP1-47708-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of HT29 cells using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0010.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human endometrium tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0012.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunoprecipitation-NBP1-47708-img0014.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/nbp1-47708_mouse-alphab-crystallin-cryab-mab-oti6a9-17720241044629.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0002.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunofluorescent staining of HT29 cells using AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0004.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454), and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70454-img0008.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-70454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0003.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunoprecipitation (IP) of CRYAB by using a monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70454-img0007.jpg)
![Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000](http://images.novusbio.com/fullsize/IL6-Antibody-3G9-Western-Blot-NBP1-47810-img0002.jpg "Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000")
![Immunocytochemistry/Immunofluorescence: IL-6 Antibody (3G9) [NBP1-47810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL6.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP1-47810-img0019.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0034.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0021.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0025.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human breast tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0027.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human colon tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0028.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0029.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Kidney tissue using anti-IL6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-3G9-Immunohistochemistry-Paraffin-NBP1-47810-img0030.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720249362622.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003225.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003219.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241003236.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241044651.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-1772024936263.jpg)
![Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-17720241044633.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)](http://images.novusbio.com/fullsize/nbp1-47810_mouse-il-6-mab-oti3g9-1772024959423.jpg)
![Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Western-Blot-NBP2-67767-img0008.jpg "Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunocytochemistry-Immunofluorescence-NBP2-67767-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunocytochemistry-Immunofluorescence-NBP2-67767-img0001.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0002.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0003.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ATF5-Antibody-SD2099-Immunohistochemistry-Paraffin-NBP2-67767-img0004.jpg)
![Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Western-Blot-H00011151-M01-img0007.jpg "Western Blot: Coronin-1a Antibody (4G10) [H00011151-M01] - CORO1A monoclonal antibody (M01), clone 4G10 Analysis of CORO1A expression in K-562.")
![Immunohistochemistry-Paraffin: Coronin-1a Antibody (4G10) [H00011151-M01] - Analysis of monoclonal antibody to CORO1A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Immunohistochemistry-Paraffin-H00011151-M01-img0005.jpg)
![Sandwich ELISA: Coronin-1a Antibody (4G10) [H00011151-M01] - Detection limit for recombinant GST tagged CORO1A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-4G10-Sandwich-ELISA-H00011151-M01-img0006.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Western-Blot-NBP1-97494-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10ug/ml.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Immunohistochemistry-Paraffin-NBP1-97494-img0003.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)