| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44493 | |||
| Azide and BSA Free | NBP2-34718-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54548-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
| IHC-Prediluted | NBP2-48351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27177SS | |||
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31350-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunohistochemistry-Paraffin-NBP1-31350-img0005.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/MCM7-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31350-img0008.jpg)
![Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunoprecipitation-NBP1-31350-img0007.jpg)
![Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-212202416030..jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518243316.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-141120251843245.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518381712.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518273411.jpg)
![Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Loss of Elf3 preserves the mesenchymal state. Immunoblot showing ELF3 and E-cadherin protein levels in response to Elf3 silencing by siRNAs. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/8/858) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Western-Blot-NBP1-30873-img0010.jpg "Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Loss of Elf3 preserves the mesenchymal state. Immunoblot showing ELF3 and E-cadherin protein levels in response to Elf3 silencing by siRNAs. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/8/858) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: ELF3/ESE-1 Antibody [NBP1-30873] - Methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30873-img0006.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - Human lung carcinoma. ESE1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30873-img0009.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - NCI-N87 xenograft, using ESE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30873-img0007.jpg)
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody [NBP1-30873] - The transcription factor ELF3 regulates the Grhl3 promoter. (A) The sequence of the Grhl3 promoter (1000 bp) showing the location of the predicted TATA box, SP1 sequence, & the transcription start site. Sequences in red represent the conserved regions, & green bases represent putative ELF3 binding sites. (B) Luciferase reporter assay showing the activation of the Grhl3 promoter by ELF3. Relative luciferase activity is calculated as fold induction relative to vector. Drawings on the left side show a schematic representation of the Grhl3 promoter region (top) & the structure of each reporter plasmid (below). The conserved regions are shown as horizontal boxes labeled 1,2 & 3, the restriction enzymes used to generate the reporter deletions of the promoter are labeled. (C) ELF3 binding at the promoter of Grhl3. NMuMG cells were treated with either vehicle or TGF beta 3 for 72 h, TGF beta 3 treated cells were then washed with PBS & continued incubation for an additional 48 h to initiate MET (post-treatment: PT48). Cells were cross-linked, & chromatin immunoprecipitation was performed. ChIP DNA was used in qPCR to measure the occupancy of ELF3 compared to control antibodies. (D) Sequence alignment of one putative ELF3 binding site in the Grhl3 promoter showing conservation among different species. In B & C, data represent the averages of at least three independent experiments. The paired Student’s t-test was used to calculate statistical significance. ** p-value < 0.01; **** p-value < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31404945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30873_rabbit-polyclonal-elf3-ese-1-antibody-310202416182629.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ESE1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30873_rabbit-polyclonal-elf3-ese-1-antibody-161020241941455.jpg)
![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Immunocytochemistry-Immunofluorescence-NBP2-32889-img0005.jpg "Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Formalin-paraffin human colon stained with p27 MAb (SX53G8)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Immunohistochemistry-Paraffin-NBP2-32889-img0001.jpg)
![Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Flow Cytometry of human p27/Kip1 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled p27/Kip1 Mouse Monoclonal Antibody (SX53G8).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Flow-Cytometry-NBP2-32889-img0007.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Formain-fixed, paraffin-embedded human prostate Carcinoma stained with p27 Monoclonal Antibody (sx53G8)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Immunohistochemistry-Paraffin-NBP2-32889-img0002.jpg)
![Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Analysis of PFA-fixed MCF-7 cells using p27/Kip1 Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-SX53G8-Flow-Cytometry-NBP2-32889-img0006.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - p27/Kip1 antibody in CODEX stain of human bone marrow tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-32889_mouse-monoclonal-p27-kip1-antibody-sx53g8-17720249362623.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19457-img0019.jpg "Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19457] - Mouse liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Frozen: N-Cadherin Antibody [NBP2-19457] - Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Frozen-NBP2-19457-img0007.jpg)
![N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody (NBP2-19457) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19457_rabbit-polyclonal-n-cadherin-antibody-immunohistochemistry-frozen-2611202518224321.jpg)
![Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Western-Blot-NB100-1483-img0001.jpg "Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - HepG2 cells were treated with DMSO, 2uM RA190 for or 25uM MG132 for 30min. The cells were fixed, permeabilized and stained for proteasome. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32375699/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1483-img0004.jpg)
![Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Flow-Cytometry-NB100-1483-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1483-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - RA190 causes accumulation of I kappa B alpha & NF-kappa B in the cytoplasm, co-localized with proteasomes. HepG2 cells were treated with DMSO, 2 μM RA190 for or 25 μM MG132 for 30 min. The cells were fixed, permeabilized & stained for I kappa B alpha a, proteasome b, NF-kappa B c & viewed by confocal fluorescence microscopy individually, or d merged, or e under phase contrast. a, b, c I kappa B alpha & NF-kappa B in RA190 & MG132-treated cells is significantly accumulated in cytoplasm, & apparently co-localized with the proteasome. The proportion of fluorescence in the cytoplasm versus the nucleus was quantified for f I kappa B alpha , or g NF-kappa B Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32375699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1483_goat-polyclonal-proteasome-19s-10b-antibody-imgenex-img-3751-310202415171319.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0003.jpg "Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP2-37563-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0013.jpg)
![Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Flow-Cytometry-NBP2-37563-img0005.jpg)
![Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Western-Blot-NBP2-37563-img0002.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0010.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-Immunohistochemistry-Paraffin-NBP2-37563-img0012.jpg)
![ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Prohibitin-Antibody-5H7-ELISA-NBP2-37563-img0001.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Knockout-Validated-NBP2-53318-img0005.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0002.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0001.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (1.3.3.22) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0006.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Immunohistochemistry-Paraffin-NBP2-44525-img0006.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).")
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Simple Western lane view shows a specific band for p21/CIP1/CDKN1A in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0002.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Electropherogram image of the corresponding Simple Western lane. p21/CIP1/CDKN1A antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0003.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720249362616.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Lane view shows a specific band for p21/CIP1/CDKN1A using 2 mg/mL of MCF-7 cell lysate and 10 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720241044618.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0011.jpg "Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.")
![Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32775-img0013.jpg)
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-Paraffin-NBP1-32775-img0004.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0005.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat 2 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0007.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0009.jpg)
![Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775] - Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of MAD1 antibody. Western blot analysis was performed using MAD1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NBP1-32775-img0015.jpg)
![Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/AIF-Antibody-Knockout-Validated-NBP2-27177-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF.](http://images.novusbio.com/fullsize/AIF-Antibody-Western-Blot-NBP2-27177-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-27177] - Staining of AIF in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. Hematoxylin-eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-27177-img0002.jpg)
![Simple Western: AIF Antibody [NBP2-27177] - Simple Western lane view shows a specific band for AIF in 0.2 mg/ml of Jurkat and MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Simple-Western-NBP2-27177-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
