| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73555 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70399 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71860 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73627 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76499-0.025mg | |||
![Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0005.jpg "Western Blot: TPX2 Antibody [NB500-184] - Depletion of TPX2 causes defects in 53BP1 ionizing radiation-induced foci formation. The protein level of 53BP1 is not affected by miRNA-mediated depletion of TPX2. Levels of actin were used as loading controls. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TPX2 Antibody [NB500-184] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-184. TPX2 was also immunoprecipitated using NB500-183 at 0.4 ug/mg lysate.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels. TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-184-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin and impacts the DAPI staining pattern and H4K16ac levels Depletion of TPX2 by siRNA causes a decrease in H4K16ac levels whereas the levels of H3K9ac and H3K56ac remain unchanged. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Knockdown-Validated-NB500-184-img0004.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416182636.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416161844.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - SIRT1 modulates the levels of H4K16ac & gamma -H2AX & is in complex with TPX2.(A) siRNA-mediated loss of SIRT1 in HeLa cells increases H4K16ac levels & decreases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (B) Overexpression of SIRT1 in MCF7 cells decreases H4K16ac levels & increases ionizing radiation-dependent gamma -H2AX levels when compared to controls. (C) Co-immunoprecipitations with TPX2 antibodies from HeLa cells with & without ionizing radiation treatment as indicated & in the absence or presence of ethidium bromide (EtBr). (The Input for TPX2 is from a longer exposure of the same blot.) (D) Co-immunoprecipitations with SIRT1 antibodies from HeLa cells with & without ionizing radiation treatment as indicated. Beads without antibodies (No Ab) were used as controls. Cells were treated with 10 Gy (or left untreated) & harvested after 1h recovery (A-B) or as indicated (C-D). See text for details. Levels of H2AX & H4 were used as loading controls. IR: ionizing radiation. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416171433.jpg)
![Western Blot: TPX2 Antibody [NB500-184] - TPX2 is constitutively associated with chromatin & impacts the DAPI staining pattern & H4K16ac levels.(A) Although the majority of TPX2 is found in the soluble fraction (see Material & Methods), a small but clearly detectable sub-population of TPX2 constitutively associates with stringent chromatin fractions obtained from MCF7 cells (left panel) or HeLa cells (right panel). These chromatin fractions contain histones but not nuclear LaminB. Upon expression of an inducible TPX2 targeting miRNA (or upon transfection with siRNA; see D) the protein was depleted from chromatin fractions. Ctrl: control cells with no induction of TPX2 miRNA. (B) TPX2 gets enriched in chromatin fractions isolated from HeLa cells after treatment with 10 Gy of ionizing radiation. Note the constitutive association of TPX2 with the chromatin in non-irradiated cells. Levels of H2AX were used as a loading control. (C) Overexpression of GFP-TPX2 or His-TPX2 causes abnormal DAPI staining in MCF7 cells compared to surrounding non-transfected cells or cells transfected with GFP. This is indicative of changes in chromatin structure. Enlargements of white frames are shown. In agreement with previous reports, overexpressed TPX2 is mostly found in the nucleus but also associates with the cytoskeleton [2]. (D-F) Depletion of TPX2 by siRNA (D) or miRNA (F) causes a decrease in H4K16ac levels whereas the levels of H3K9ac & H3K56ac remain unchanged. (E) Quantification of H3K9ac & H3K56ac levels from MCF7 cells transfected with control or TPX2 siRNA (n = 4 independent experiments each; p(t test)>0.05; NS: non significant; Error bars represent SE). Stripping of western blots & re-development with antibodies specific for H3 & H4 ensured equal loading. See text for details. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0110994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-184_rabbit-polyclonal-tpx2-antibody-310202416173534.jpg)
![Western Blot: PP5 Antibody (2E12) [NBP1-47927] Analysis of extracts (35ug) from 9 different cell lines by using anti-PP5 monoclonal antibody.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Western-Blot-NBP1-47927-img0007.jpg "Western Blot: PP5 Antibody (2E12) [NBP1-47927] Analysis of extracts (35ug) from 9 different cell lines by using anti-PP5 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: PP5 Antibody (2E12) [NBP1-47927] - Immunofluorescent staining of HT29 cells using anti-PP5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Immunocytochemistry-Immunofluorescence-NBP1-47927-img0003.jpg)
![Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human pancreas tissue using anti-PP5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Immunohistochemistry-Paraffin-NBP1-47927-img0006.jpg)
![Flow Cytometry: PP5 Antibody (2E12) [NBP1-47927] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PP5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Flow-Cytometry-NBP1-47927-img0001.jpg)
![Western Blot: PP5 Antibody (2E12) [NBP1-47927] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PP5.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Western-Blot-NBP1-47927-img0002.jpg)
![Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human endometrium tissue using anti-PP5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Immunohistochemistry-Paraffin-NBP1-47927-img0004.jpg)
![Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human Ovary tissue using anti-PP5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PP5-Antibody-2E12-Immunohistochemistry-Paraffin-NBP1-47927-img0005.jpg)
![Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Western-Blot-NBP2-01245-img0003.jpg "Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.")
![Immunocytochemistry/Immunofluorescence: CDKN3 Antibody (2E11) [NBP2-01245] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunocytochemistry-Immunofluorescence-NBP2-01245-img0009.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human prostate tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0008.jpg)
![Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Flow-Cytometry-NBP2-01245-img0002.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0004.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0005.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0006.jpg)
![Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human Kidney tissue using anti-CDKN3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Immunohistochemistry-Paraffin-NBP2-01245-img0007.jpg)
![Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - Analysis of Jurkat cells, using anti-CDKN3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDKN3-Antibody-2E11-Flow-Cytometry-NBP2-01245-img0001.jpg)
![Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Western-Blot-NBP2-01128-img0008.jpg "Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] Analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-01128-img0007.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Human lymphoma tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0006.jpg)
![Flow Cytometry: ANAPC2 Antibody (1A6) [NBP2-01128] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANAPC2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Flow-Cytometry-NBP2-01128-img0001.jpg)
![Western Blot: ANAPC2 Antibody (1A6) [NBP2-01128] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Western-Blot-NBP2-01128-img0002.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0003.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0004.jpg)
![Immunohistochemistry-Paraffin: ANAPC2 Antibody (1A6) [NBP2-01128] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ANAPC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ANAPC2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01128-img0005.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0007.jpg "Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunocytochemistry-Immunofluorescence-NBP1-47754-img0006.jpg)
![Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-OTI1F10-Immunohistochemistry-NBP1-47754-img0008.jpg)
![Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Flow-Cytometry-NBP1-47754-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0002.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0004.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0005.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0020.jpg "Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) [NBP1-47695] - Chk2 Antibody (5C4) [NBP1-47695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunocytochemistry-Immunofluorescence-NBP1-47695-img0013.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0019.jpg)
![Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Flow-Cytometry-NBP1-47695-img0012.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0011.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0014.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0015.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0016.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0017.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0018.jpg)
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Western-Blot-NBP2-00990-img0019.jpg "Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunocytochemistry-Immunofluorescence-NBP2-00990-img0011.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0018.jpg)
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S 10B monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Western-Blot-NBP2-00990-img0010.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0012.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0013.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0014.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0015.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0016.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0017.jpg)
![Western Blot: HGF Antibody (7-2) [NBP1-19182] - Lane 1 hHGF 1ug, Lane 2 hHGF 0.5ug and Lane 3 hHGF 0.25ug.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Western-Blot-NBP1-19182-img0008.jpg "Western Blot: HGF Antibody (7-2) [NBP1-19182] - Lane 1 hHGF 1ug, Lane 2 hHGF 0.5ug and Lane 3 hHGF 0.25ug.")
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Classification of liposarcomas generated in the Ptenflox/flox; p53flox/flox mouse model. Different subtypes of liposarcoma are characterized by a unique lipocytic phenotype. Lipoprotein lipase (LPL) is an adipocytic marker and stains more mature adipocytic sarcomas such as WDLPS and MLPS. Hepatocyte growth factor (HGF) is a mesenchymal stem cell marker and stains immature liposarcomas such as DDLPS and PLPS. Scale bar corresponds to 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cdd.2015.27) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0009.jpg)
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Human breast tissue stained with HGF/SF, mAb (7-2) at 10 ug/ml.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0005.jpg)
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Human breast tissue stained with HGF/SF, mAb (7-2) at 10ug/ml.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0007.jpg)
![Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Western-Blot-NB100-74551-img0009.jpg "Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .")
![Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0005.jpg)
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Normal biopsies of deparaffinized Human prostate tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis of TIMP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0004.jpg)
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0007.jpg)
![Western Blot: SLC39A2/ZIP2 Antibody [NBP1-76499] - Analysis of ZIP2 in (A) mouse and (B) rat brain tissue lysate with ZIP2 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/SLC39A2-ZIP2-Antibody-Western-Blot-NBP1-76499-img0004.jpg "Western Blot: SLC39A2/ZIP2 Antibody [NBP1-76499] - Analysis of ZIP2 in (A) mouse and (B) rat brain tissue lysate with ZIP2 antibody at 1 ug/mL.")
![Immunohistochemistry: SLC39A2/ZIP2 Antibody [NBP1-76499] - SCL39A2 (1:200) from equine placenta. Heat based antigen retrieval using a EDTA based buffer for 10 min. Fetal part of the placenta displays stronger staining than the maternal part. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/SLC39A2-ZIP2-Antibody-Immunohistochemistry-NBP1-76499-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: SLC39A2/ZIP2 Antibody - BSA Free [NBP1-76499] - Immunofluorescence of SLC39A2/ZIP2 in human brain tissue with SLC39A2/ZIP2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76499_rabbit-polyclonal-slc39a2-zip2-antibody-271120241937753.jpg)
![Immunohistochemistry: SLC39A2/ZIP2 Antibody - BSA Free [NBP1-76499] - Immunohistochemistry of SLC39A2/ZIP2 in human brain tissue with SLC39A2/ZIP2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76499_rabbit-polyclonal-slc39a2-zip2-antibody-2711202419562150.jpg)
![Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-FC82291-Western-Blot-NB600-1030-img0001.jpg "Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse")
![Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunofluorescence-NB600-1030-img0002.jpg)
![Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunoblotting-NB600-1030-img0008.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Western-Blot-NBP1-51643-img0010.jpg "Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.")
![Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-NBP1-51643-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Flow-Cytometry-NBP1-51643-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-Paraffin-NBP1-51643-img0009.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 promoted the expression of HK2 in CRC cells.a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) & RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) & RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 & RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. beta -actin served as a loading control. f HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 & RKO cells. beta -actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202416173538.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 regulated glycolysis through HK2.a The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta -actin served as a loading control. b, c Glucose consumption (b) & lactate production (c) were measured in B7-H3-overexpressing HCT116 or RKO cells transfected with NC or HK2 siRNA. d, e Glucose consumption (d) & lactate production (e) were measured in B7-H3-overexpressing HCT116 or RKO cells treated with PBS or 2-DG. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202415175275.jpg)