| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33240 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54551-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27072SS | |||
![Western Blot: HGF Antibody (7-2) [NBP1-19182] - Lane 1 hHGF 1ug, Lane 2 hHGF 0.5ug and Lane 3 hHGF 0.25ug.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Western-Blot-NBP1-19182-img0008.jpg "Western Blot: HGF Antibody (7-2) [NBP1-19182] - Lane 1 hHGF 1ug, Lane 2 hHGF 0.5ug and Lane 3 hHGF 0.25ug.")
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Classification of liposarcomas generated in the Ptenflox/flox; p53flox/flox mouse model. Different subtypes of liposarcoma are characterized by a unique lipocytic phenotype. Lipoprotein lipase (LPL) is an adipocytic marker and stains more mature adipocytic sarcomas such as WDLPS and MLPS. Hepatocyte growth factor (HGF) is a mesenchymal stem cell marker and stains immature liposarcomas such as DDLPS and PLPS. Scale bar corresponds to 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cdd.2015.27) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0009.jpg)
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Human breast tissue stained with HGF/SF, mAb (7-2) at 10 ug/ml.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0005.jpg)
![Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - Human breast tissue stained with HGF/SF, mAb (7-2) at 10ug/ml.](http://images.novusbio.com/fullsize/HGF-Antibody-7-2-Immunohistochemistry-Paraffin-NBP1-19182-img0007.jpg)
![Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19438-img0009.jpg "Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0010.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-9102024205611.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.mtTFA stained by mtTFA antibody (NBP2-19438) diluted at 1:165.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-91020242014635.jpg)
![Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Western-Blot-NBP2-53354-img0006.jpg "Western Blot: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Confocal Immunofluorescence of MCF-7 cells E-Cadherin Antibody (CDH1/1525). labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunocytochemistry-Immunofluorescence-NBP2-53354-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human breast adenocarcinoma stained with E-Cadherin Mouse Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0009.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0001.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Immunohistochemistry-Paraffin-NBP2-53354-img0004.jpg)
![Protein Array: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E-Cadherin Antibody (CDH1/1525).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-1525-Protein-Array-NBP2-53354-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/1525) [NBP2-53354] - Human breast cancer tissue stained fro E-cadherin (white) and counterstained with DAPI (blue). Alexa Fluor 750 version of antibody used. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53354_mouse-monoclonal-e-cadherin-antibody-cdh1-1525-17720241044616.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-27072-img0004.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-27072-img0005.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-27072-img0002.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25153-img0001.jpg "Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25153-img0003.jpg)
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-214-img0003.jpg "Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (50 ug) from TCMK-1 and BIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-214-img0002.jpg)
![Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody (NB100-214, lot 4) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot 3). For blotting immunoprecipitated BLM, NB100-214 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunoprecipitation-NB100-214-img0001.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM5-Antibody-Western-Blot-NB100-79778-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-NB100-79778-img0010.jpg)
![Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Western-Blot-NB500-191-img0010.jpg "Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot NB500-191-4) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-191-3). For blotting immunoprecipitated XRN1, NB500-191 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Xrn1-Antibody-Immunoprecipitation-NB500-191-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![<b>Independent Antibodies Validation. </b>Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)