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![Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of AIF-1/Iba1 protein from K562 whole cell extracts using 5ug of AIF-1/Iba1 Antibody Western blot analysis was performed using Iba1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunoprecipitation-NBP2-16908-img0018.jpg "Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of AIF-1/Iba1 protein from K562 whole cell extracts using 5ug of AIF-1/Iba1 Antibody Western blot analysis was performed using Iba1 antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16908-img0041.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0039.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0032.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse cerebellum. Iba1 stained by Iba1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0033.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat cerebellum. Iba1 stained by Iba1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0034.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0035.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0036.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Rat brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0040.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0038.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0014.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16908_rabbit-polyclonal-aif-1-iba1-antibody-910202419593573.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16908_rabbit-polyclonal-aif-1-iba1-antibody-91020242015719.jpg)
![Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Western-Blot-NB500-207-img0001.jpg "Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.")
![Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Immunocytochemistry-Immunofluorescence-NB500-207-img0004.jpg)
![Western Blot: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of KLF11 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/KLF11-Antibody-10D8-Western-Blot-H00008462-M03-img0007.jpg "Western Blot: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of KLF11 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of monoclonal antibody to KLF11 on NIH/3T3 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/KLF11-Antibody-10D8-Immunocytochemistry-Immunofluorescence-H00008462-M03-img0005.jpg)
![Immunohistochemistry-Paraffin: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of monoclonal antibody to KLF11 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/KLF11-Antibody-10D8-Immunohistochemistry-Paraffin-H00008462-M03-img0006.jpg)
![Chromatin Immunoprecipitation: KLF11 Antibody (10D8) [H00008462-M03] - 5 ug KLF11 antibody on human adipocytes - data from a customer review.](http://images.novusbio.com/fullsize/KLF11-Antibody-10D8-Chromatin-Immunoprecipitation-H00008462-M03-img0004.jpg)
![Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Western-Blot-H00004218-M02-img0003.jpg "Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: RAB8A Antibody (3G1) [H00004218-M02] - Neuromast hair cells of zebrafish embryos stained with RAB8A antibody. Donkey anti-mouse Alexa Fluor 594 secondary antibody was used. Image from verified customer.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Immunocytochemistry-Immunofluorescence-H00004218-M02-img0005.jpg)
![ELISA: RAB8A Antibody (3G1) [H00004218-M02] - Detection limit for recombinant GST tagged RAB8A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-ELISA-H00004218-M02-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0010.jpg "Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cdk4-Antibody-Immunohistochemistry-Paraffin-NBP1-31308-img0006.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0009.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024205643.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024209146.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-910202420104465.jpg)
![Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP alpha.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Western-Blot-NBP2-01026-img0024.jpg "Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP alpha.")
![Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP alpha.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunocytochemistry-Immunofluorescence-NBP2-01026-img0015.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0023.jpg)
![Flow Cytometry: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRAP alpha antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Flow-Cytometry-NBP2-01026-img0001.jpg)
![Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP alpha monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Western-Blot-NBP2-01026-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Immunofluorescent staining of HeLa cells using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunocytochemistry-Immunofluorescence-NBP2-01026-img0014.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0016.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0017.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0018.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0019.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0020.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0021.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human pancreas tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0022.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Western-Blot-NB300-560-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0007.jpg "Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; red). Nucleus is stained with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0001.jpg)
![Immunoprecipitation: alpha Tubulin Antibody (TU-01) [NB500-333] - alpha Tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Molecular weight of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunoprecipitation-NB500-333-img0006.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of alpha-tubulin (molecular weight of 50 kDa) in porcine brain lysate by antibody TU-01 biotin.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Tubulin filament in HeLa. Image from verified customer review.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0005.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of lysates of various cell lines by a newly developed monoclonal antibody (B). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0002.jpg)
![Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-H00124404-B01P-img0006.jpg "Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - An IFA revealed multiple localizations of SEPT12 and LAMINB1 signals. A total of 32 male germ cells were were evaluated as (a.) Round spermatids (n = 9; labeled with anti-SEPT12 and anti-LAMINB1 antibodies as the presented figure,9/9); (b.) elongating and (c.) elongated spermatids (n = 11;11/11); (d.) ejaculated spermatozoa (n = 10;10/10). (a.-d.) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), and a combination of the left and middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0007.jpg)
![Immunohistochemistry-Paraffin: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on formalin-fixed paraffin-embedded human colon. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunohistochemistry-Paraffin-H00124404-B01P-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - SEPT12 interacts & colocalizes with LAMINB1 male germ cells.(A) Co-IP of FLAG-SPAG4 & LAIMINB1 with SEPT12-GFP. The cell lysates of the NT2D1 cells, transfected with pEGFP-SEPT12 & a pFLAG-SPAG4 vector, are subjected to IP with an anti-GFP antibody (right panel, Row 3) or a nonspecific control IgG (right panel, Row 2), followed by IB with an anti-GFP, anti-FLAG, or anti-LAMINB1 antibody. An input protein (5%) was used as a control during the IB of the transfected cell lysates (right panel, Row 1). (B) An IFA revealed multiple localizations of SEPT12 & LAMINB1 signals. A total of 32 male germ cells were were evaluated as (A) Round spermatids (n = 9; labeled with anti-SEPT12 & anti-LAMINB1antibodies as the presented figure,9/9); (B) elongating & (C) elongated spermatids (n = 11;11/11); (D) ejaculated spermatozoa (n = 10;10/10). (A–D) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), & a combination of the left & middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00124404-b01p_mouse-polyclonal-septin-12-antibody-310202415483631.jpg)
![Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4C4-Western-Blot-NBP2-25151-img0006.jpg "Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. NBP2-25151 antibody specifically labels the nuclear lamina, while the HSP60 antibody reveals protein expressed in mitochondria.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4C4-Immunocytochemistry-Immunofluorescence-NBP2-25151-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25151 reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4C4-Immunocytochemistry-Immunofluorescence-NBP2-25151-img0004.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: SSBP1 Antibody [NBP1-80720] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SSBP1 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Western-Blot-NBP1-80720-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SSBP1 Antibody [NBP1-80720] - Mitochondrial components segregate into MVBs and are secreted in exosomes. Confocal co-localization analysis in HEK293 cells treated with FCCP (4 h) and stained with SSBP1 (red), EEA1 and CD63 (green). Bar, 10 um. Graph shows means for Mander's co-localization coefficient for SSBP1 and EEA1 or CD63 (n = 3). t-test: ***P-value < 0.0001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05077-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80720-img0023.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0020.jpg)
![Western Blot: SSBP1 Antibody [NBP1-80720] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Western-Blot-NBP1-80720-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SSBP1 Antibody [NBP1-80720] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80720-img0010.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human cerebral cortex shows granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0017.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0018.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0019.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group P<0.05. #Compared with the DM group P<0.05. DM indicates diabetes mellitus; NS, not significant; TFAM, mitochondrial transcription factor A. Image collected and cropped by CiteAb from the following publication (www.ahajournals.org/doi/10.1161/JAHA.118.008807) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19437-img0008.jpg "Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group P<0.05. #Compared with the DM group P<0.05. DM indicates diabetes mellitus; NS, not significant; TFAM, mitochondrial transcription factor A. Image collected and cropped by CiteAb from the following publication (www.ahajournals.org/doi/10.1161/JAHA.118.008807) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human colon cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0007.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human esophagus cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0006.jpg)
![Immunoprecipitation: mtTFA Antibody [NBP2-19437] - A. 293T whole cell lysate/extract B. 40 ug 293T whole cell lysate/extract. C.. Control with 2 ug of preimmune rabbit IgG.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunoprecipitation-NBP2-19437-img0004.jpg)
![HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mtTFA stained by mtTFA antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-19437_rabbit-polyclonal-mttfa-antibody-immunofluorescence-22320239914.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19437) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-910202420115071.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP2-19437] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-1610202419414569.jpg)