| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24635SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24722SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33518-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-91761SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0010.jpg "Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.")
![Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of skin, epidermis using ARNT/HIF-1 beta antibody [NB100-110] from the ARNT/HIF-1 beta antibody pack [NB100-982].](http://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0005.jpg)
![Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and ARNT/HIF-1 beta) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and ARNT/HIF-1 beta antibody [NB100-110] immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-982-img0009.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1a or HIF-2a expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1a-shRNA or HIF-2a-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. ARNT/HIF-1 beta expression was used as a loading control. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0006.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A [NB100-480] and ARNT/HIF-1 beta [NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0007.jpg)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of human glioblastoma multi-forme utilizing the ARNT/HIF-1 beta antibody[NB100-124] from the ARNT/HIF-1 beta antibody pack [NB100-982].](http://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0004.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)
![Western Blot: KLF15 Antibody [NBP2-24635] - Analysis of KLF15 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24635 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/KLF15-Antibody-Western-Blot-NBP2-24635-img0001.jpg "Western Blot: KLF15 Antibody [NBP2-24635] - Analysis of KLF15 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24635 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: KLF15 Antibody [NBP2-24635] - KLF15 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/KLF15-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24635-img0006.jpg)
![Immunohistochemistry-Paraffin: KLF15 Antibody [NBP2-24635] - Tissue section of human liver using KLF15 antibody at 1:100 dilution. The antibody generated strong cytoplasmic staining with some nuclear signal in the hepatocytes, while in Kupffer cells, the signal was mainly nuclear and was most intense among all cell types.](http://images.novusbio.com/fullsize/KLF15-Antibody-Immunohistochemistry-Paraffin-NBP2-24635-img0002.jpg)
![Western Blot: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Western-Blot-NBP2-24722-img0002.jpg "Western Blot: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of human adrenal using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-24722-img0003.jpg)
![Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-24722-img0001.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0018.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0015.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0016.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0017.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Analysis in human stomach and skeletal muscle tissues. Corresponding Lysozyme RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Analysis in human stomach and skeletal muscle tissues. Corresponding Lysozyme RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lysozyme Antibody [NBP2-33518] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP2-33518-img0005.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human stomach shows moderate extracellular positivity in glandular cells.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-NBP2-33518-img0016.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human kidney shows strong extracellular positivity in cells in tubules.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0013.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human lymph node shows moderate extracellular positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0014.jpg)
![Immunohistochemistry-Paraffin: Lysozyme Antibody [NBP2-33518] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Immunohistochemistry-Paraffin-NBP2-33518-img0015.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining in human skin and pancreas tissues using anti-POSTN antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82472-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining in human skin and pancreas tissues using anti-POSTN antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Western-Blot-NBP1-82472-img0005.jpg)
![Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82472-img0006.jpg)
![Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82472-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Characteristics of lung cancer cell lines according to Periostin/OSF-2 (POSTN) expression. Comparison of Periostin/OSF-2 expression levels detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/23/3/1240) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Knockdown-Validated-NBP1-82472-img0009.jpg)
![Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-82472] - Section of non-small cell lung carcinoma (NSCLC) immunostained for Periostin/OSF-2 in cancer-associated fibroblasts (CAFs). Magnification 50×. Bar = 200 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32987711/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-82472_rabbit-periostin-osf-2-pab-20920231742123.jpg)
![Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - The efficacy of shRNA lentiviral particles transfection. (A) Silencing of periostin (POSTN) expression was confirmed by Western blot analysis. beta -actin was used as an internal control. (B) Confocal images & fluorescence analysis showing the expression of POSTN in control A549.CTRL cells & A549.shRNA cells (*** p 0.05). Objective 60×/1.40 Oil; pinhole airy 1.25. Bar = 20 µm. (C) Immunohistochemical expression level of periostin (POSTN) in the tissue of particular non-small cell lung carcinoma (NSCLC) subtypes: squamous cell carcinoma (SCC) & adenocarcinoma (AC) (p > 0.05). IRS, immunoreactive score. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35163164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82472_rabbit-periostin-osf-2-pab-310202415392583.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg "Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).")
![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg)
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of human placenta tissue lysate using Factor VIII antibody at 1:500.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0002.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 with ECL based detection.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0006.jpg)
![Western Blot: CCDC109A Antibody [NBP1-88534] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCU antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Western-Blot-NBP1-88534-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CCDC109A Antibody [NBP1-88534] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCU antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0014.jpg)
![Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0008.jpg)
![Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0009.jpg)
![Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human hippocampus shows moderate to strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0010.jpg)
![Simple Western: CCDC109A Antibody [NBP1-88534] - Simple Western lane view shows a specific band for MCU in 0.2 mg/ml of RT-4 (left), A549 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Simple-Western-NBP1-88534-img0005.jpg)
![Simple Western: CCDC109A Antibody [NBP1-88534] - Electropherogram image(s) of corresponding Simple Western lane view. CCDC109A antibody was used at 1:20 dilution on RT-4 and A549 lysate(s).](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Simple-Western-NBP1-88534-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)
![Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)