| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56326SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56343SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-92756SS | |||
![Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0008.jpg "Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DDX3 Antibody [NB200-194] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-194 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-195 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0005.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescence (A120-601D4) and DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0006.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of mouse DDX3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX3 Antibody [NB200-194] - Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody NB200-194 (lot NB200-194-2) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (lot NB200-194-1) and rabbit anti-DDX3 antibody NB200-196. For blotting immunoprecipitated DDX3, NB200-194 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunoprecipitation-NB200-194-img0007.jpg)
![Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB100-56326-img0002.jpg "Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56326-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0007.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0006.jpg)
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Western Blot: ABAT Antibody [NBP2-21598] - 30 ug SK-N-SH whole cell lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP2-21598-img0001.jpg "Western Blot: ABAT Antibody [NBP2-21598] - 30 ug SK-N-SH whole cell lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.")
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse kidney. ABAT antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP2-21598-img0007.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - ABAT antibody detects ABAT protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.ABAT stained by ABAT antibody (NBP2-21598) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-21598_rabbit-polyclonal-abat-antibody-910202420134032.jpg)
![ABAT antibody detects ABAT protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: ABAT protein stained by ABAT antibody (NBP2-21598) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-21598_rabbit-polyclonal-abat-antibody-immunohistochemistry-frozen-261120251829491.jpg)
![ABAT antibody detects ABAT protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: ABAT protein stained by ABAT antibody (NBP2-21598) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-21598_rabbit-polyclonal-abat-antibody-immunohistochemistry-frozen-26112025185045.jpg)
![Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Western-Blot-NB100-56462-img0004.jpg "Western Blot: Smad2 Antibody [NB100-56462] - Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with this antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Smad2 Antibody [NB100-56462] - Mouse testis tissue probed with this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SMAD2-Antibody-Immunohistochemistry-Paraffin-NB100-56462-img0003.jpg)
![Western Blot: Alas1 Antibody [NB100-56415] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml.](http://images.novusbio.com/fullsize/Alas1-Antibody-Western-Blot-NB100-56415-img0003.jpg "Western Blot: Alas1 Antibody [NB100-56415] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml.")
![Immunohistochemistry-Paraffin: Alas1 Antibody [NB100-56415] - Staining of human skin at 5 ug/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer was used for unmasking. A biotinylated secondary antibody followed by alkaline phosphatase-streptavidin was and chromogen was used for detection.](http://images.novusbio.com/fullsize/Alas1-Antibody-Immunohistochemistry-Paraffin-NB100-56415-img0004.jpg)
![Simple Western: Alas1 Antibody [NB100-56415] - Simple Western lane view shows a specific band for Alas1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Alas1-Antibody-Simple-Western-NB100-56415-img0005.jpg)
![Western Blot: MTA2 Antibody [NB100-56483] - Lysate from Jurkat cells, in the 1) absence and 2) presence of immunizing peptide, probed with this antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MTA2-Antibody-Western-Blot-NB100-56483-img0004.jpg "Western Blot: MTA2 Antibody [NB100-56483] - Lysate from Jurkat cells, in the 1) absence and 2) presence of immunizing peptide, probed with this antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: MTA2 Antibody [NB100-56483] - This antibody was tested in HEK293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/MTA2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56483-img0001.jpg)
![Immunohistochemistry: MTA2 Antibody [NB100-56483] - Analysis of Human Testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/MTA2-Antibody-Immunohistochemistry-NB100-56483-img0003.jpg)
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunohistochemistry-Paraffin-NBP2-14890-img0009.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for 15 minutes by flow cytometry analysis.Black: Unlabelled sample was used as a control.Red: BrdU antibodyAcquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Flow-Cytometry-NBP2-14890-img0004.jpg)
![Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 knockdown potently (C) inhibited cell proliferation. Error bars represent mean +/- SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 and SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, and subjected to immunoblotting and MTT assays. Bars represent mean +/- SD of three replicates (**P < 0.01). Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article/47/3/1255/5212009) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APCDD1-Antibody---BSA-Free-Knockdown-Validated-NB110-92756-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 knockdown potently (C) inhibited cell proliferation. Error bars represent mean +/- SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 and SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, and subjected to immunoblotting and MTT assays. Bars represent mean +/- SD of three replicates (**P < 0.01). Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article/47/3/1255/5212009) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: APCDD1 Antibody [NB110-92756] - Detection of APCDD1 in human heart lysate](http://images.novusbio.com/fullsize/APCDD1-Antibody-Western-Blot-NB110-92756-img0003.jpg)
![Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 is co-regulated by MEIS1 & EWS-FLI1 & mediates the oncogenic role of MEIS1. (A, B) Silencing of (A) MEIS1 or (B) EWS-FLI1 downregulated expression of both APCDD1 mRNA & protein. Error bars represent mean ± SD of three replicates (**P < 0.01, ***P < 0.001). (C, D) APCDD1 knockdown potently (C) inhibited cell proliferation & (D) decreased colony formation. Error bars represent mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 & SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, & subjected to immunoblotting & MTT assays. Bars represent mean ± SD of three replicates (**P < 0.01). (F) Proposed model showing that MEIS1 & EWS-FLI1 co-operatively activate APCDD1 transcription, thereby promoting the malignant phenotype of Ewing sarcoma cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30496486), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-92756_rabbit-polyclonal-apcdd1-antibody-310202416205184.jpg)
![Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 is co-regulated by MEIS1 & EWS-FLI1 & mediates the oncogenic role of MEIS1. (A, B) Silencing of (A) MEIS1 or (B) EWS-FLI1 downregulated expression of both APCDD1 mRNA & protein. Error bars represent mean ± SD of three replicates (**P < 0.01, ***P < 0.001). (C, D) APCDD1 knockdown potently (C) inhibited cell proliferation & (D) decreased colony formation. Error bars represent mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 & SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, & subjected to immunoblotting & MTT assays. Bars represent mean ± SD of three replicates (**P < 0.01). (F) Proposed model showing that MEIS1 & EWS-FLI1 co-operatively activate APCDD1 transcription, thereby promoting the malignant phenotype of Ewing sarcoma cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30496486), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-92756_rabbit-polyclonal-apcdd1-antibody-310202416212389.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0012.jpg "Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug mouse heart lysate/extract 12 % SDS-PAGE COX4 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0003.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug rat muscle lysate/extract 15 % SDS-PAGE COX4 antibody [854] dilution: 1:1000.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (854) [NBP2-43540] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [854]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0007.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0011.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human ovarian cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Immunohistochemistry-Paraffin-NBP2-43540-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody (854) [NBP2-43540] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-43540) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43540_mouse-monoclonal-cox4-antibody-854-910202420134064.jpg)
![COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.COX4 stained by COX4 antibody [GT854] (NBP2-43540) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43540_mouse-monoclonal-cox4-antibody-854-immunohistochemistry-paraffin-2611202518243910.jpg)
![Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Western-Blot-NBP2-15085-img0015.jpg "Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0018.jpg)
![Western Blot: RAB11B Antibody [NBP2-15085] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Western-Blot-NBP2-15085-img0013.jpg)
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Human colon carcinoma, using RAB11B antibody antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0002.jpg)
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0008.jpg)
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0011.jpg)
![Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB11B-Antibody-Immunohistochemistry-Paraffin-NBP2-15085-img0016.jpg)
![Western Blot: RAB11B Antibody [NBP2-15085] - Proteomic profiling of autophagic cargo during NMDAR-LTD.a–d WB analyses of different fractions along the autophagic vesicle purification procedure, using antibodies against a autophagosomal markers (LC3B, p62, Atg16L1, & Atg9A), b ER-Golgi markers (TGN, LMAN1, SAR1a), c endosomal markers (Rab11b, EEA1), & d markers of plasma-membrane (Stx4), extracellular vesicles (Alix) & nuclear extracts (TBP). N = 3 independent experiments. e Graph showing the cell component analysis, as false discovery rate (FDR)-corrected p-values, of dynamic cargo (total of 393 proteins) that is enriched (up) or less abundant (down) in AVs after LTD, compared to control. f Graphical representation of proteins enriched in AVs upon LTD, w/ relation to synapse. g WB analysis of PK-treated control & LTD-AVs, validating the presence of proteins identified by the proteomic analyses in autophagic vesicles. Postsynaptic density (PSD) fraction used as reference. Graph showing the fold change of normalized levels of proteins validated by WB, as a ratio of LTD to control. Cargo proteins normalized to levels of p62, which remains unaffected at the early phase of LTD. N = 3 independent AV preparations. Bars represent mean values ± SEM. Statistical analysis performed using paired, two-tailed Student’s t-test (GluA1,N = 6, P = 0.0002; GluA2, N = 6, P = 0.0039; Pick1, N = 5, P = 0.011; SAP97, N = 5, P = 0.0179; FYN, N = 8, P Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-91020242014691.jpg)
![Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-91020242014663.jpg)
![RAB11B antibody [N1C3] detects RAB11B protein by immunofluorescent analysis.Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-immunocytochemistry-immunofluorescence-26112025185046.jpg)
![RAB11B antibody [N1C3] detects RAB11B Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-immunohistochemistry-frozen-2611202518402217.jpg)
![GRAMD1B antibody [C1C2], Internal detects GRAMD1B protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min.Green: GRAMD1B protein stained by GRAMD1B antibody [C1C2], Internal (NBP2-15085) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15085_rabbit-polyclonal-rab11b-antibody-immunocytochemistry-immunofluorescence-26112025198105.jpg)
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16550_rabbit-polyclonal-fxr-nr1h4-antibody-western-blot-1220249182..jpg)
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Biochemical measurements & the mRNA & protein expression of top 5 NRs. A, Results of biochemical parameter measurements. Among the serum indicators, ALT in HFD group higher than it in NC group (P = .0004) & CSP group (P = .0222); the AST in HFD group higher than it in NC group (P = .0006) & CSP group (P = .0149); ALP in HFD group higher than it in NC group (P < .0001) & CSP group (P = .0013), & it lower in NC group than in CSP group (P = .0017); in HFD group, the TC level higher than it in NC group (P < .0001) & CSP group (P = .0003), & in NC group, it lower than it in CSP group (P < .0001); in HFD group, the TG level higher than it in NC group (P = .0011) & CSP group (P = .0476); in HFD group, the GLU level higher than it in NC group (P < .0001) & CSP group (P = .0002); & in HFD group, the HDL‐c level higher than it in NC group (P < .0001) & CSP group (P = .0022), & in NC group, it lower than it in CSP group (P < .0001) (n = 10). Among the liver indicators, in NC group, the TC level lower than it in HFD group (P = .0276) & CSP group (P = .0427); in NC group, the TG level lower than it in HFD group (P = .0427) & CSP group (P = .0448) (n = 6). B, The mRNA expression of top 5 NRs in liver. PPAR gamma in HFD group expressed at a higher level than it in NC group (P = .0072) & CSP group (P = .0489); FXR in HFD group expressed at a lower level than it in NC group (P = .0222) & CSP group (P = .0331); PPAR alpha in HFD group expressed lower than NC group (P = .0143) & CSP group (P = .0082); & RAR alpha in HFD group expressed at a higher level than it in NC group (P = .0001) & CSP group (P = .0012) (n = 10). C, Expression of top 5 NRs in liver measured by automatic WB analysis Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32189432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16550_rabbit-polyclonal-fxr-nr1h4-antibody-310202416113545.jpg)