| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83348-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0131A488-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-17780SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56585SS | |||
| Azide and BSA Free | NBP2-33232 | |||
| Azide and BSA Free | NBP2-33232-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36431SS | |||
| Azide and BSA Free | NBP2-80936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33154 | |||
| IHC-Prediluted | NBP2-44334 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36429-0.025mg | |||
| Azide and BSA Free | NBP2-81009 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13600SS | |||
| Azide and BSA Free | NBP2-80924 | |||
![Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, microtubule organizing center & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83348-img0012.jpg "Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, microtubule organizing center & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0017.jpg)
![Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Western-Blot-NBP1-83348-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney and liver tissues. Corresponding Podocalyxin Like RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0014.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0015.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-mAb89-Immunocytochemistry-Immunofluorescence-DDX0131P-100-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.")
![Immunohistochemistry: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - IHC staining on Bouin paraffinlymph node section. HumanCD40 stained with mAb 89.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-mAb89-Immunohistochemistry-DDX0131P-100-img0004.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0004.jpg "Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.")
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Western-Blot-NBP2-43648-img0005.jpg)
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 30 ug zebrafish brain lysate/extract 10 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Western-Blot-NBP2-43648-img0006.jpg)
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Western-Blot-NBP2-43648-img0007.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of H1299 xenograft. Citrate synthetase antibody dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0001.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse duodenum. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0002.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse heart. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-1761-Immunohistochemistry-Paraffin-NBP2-43648-img0003.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-261120251835176.jpg)
![Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-2611202518185010.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-261120251981024.jpg)
![Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.A. 30 ug zebrafish heart lysate/extract10% SDS-PAGECitrate synthetase antibody [GT1761] (NBP2-43648) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-2611202518451320.jpg)
![HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-western-blot-261120251822204.jpg)
![Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43648_mouse-monoclonal-cs-citrate-synthase-antibody-1761-immunocytochemistry-immunofluorescence-2611202518224317.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - Normalized expression of TGF-b mRNA in WT, EV-, or FKBP51/FKBP5-stably transfected melanoma cells. WT sample expression 1/4 1. (n 1/4). Western blot assay of TGF-b levels in the same cells. Anti-Flag-labelled exogenous FKBP51/FKBP5. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-NB100-68240-img0006.jpg "Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - Normalized expression of TGF-b mRNA in WT, EV-, or FKBP51/FKBP5-stably transfected melanoma cells. WT sample expression 1/4 1. (n 1/4). Western blot assay of TGF-b levels in the same cells. Anti-Flag-labelled exogenous FKBP51/FKBP5. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license.")
![Immunohistochemistry: FKBP51/FKBP5 Antibody [NB100-68240] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FKBP5/FKBP51 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Immunohistochemistry-NB100-68240-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - Western Blot and Immunoprecipitation: FKBP5/FKBP51 Antibody NB100-68240 - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68240 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. FKBP5/FKBP51 was also immunoprecipitated by rabbit anti-FKBP5/FKBP51 antibody NB100-68239, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Western-Blot-NB100-68240-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51/FKBP5 modulates EMT hallmarks. FKBP51/FKBP5 silencing of SAN melanoma cells decreases TGF-b and TbRIII expression. Normalized expression rates (mean +/-S.D.) of TGF-b mRNA levels; (n 1/4). FKBP51/FKBP5 siRNA-treated sample expression 1/4. 1. Western blot assay of TGF-b in SAN melanoma cells treated with nonsilencing (NS) RNA or FKBP51/FKBP5 siRNA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license./4](http://images.novusbio.com/fullsize/FKBP51-FKBP5-Antibody-Knockdown-Validated-NB100-68240-img0005.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) & ABCB5 mRNA (grey) (n=3). FKBP51-treated sample expression=1. (Right) Western blot assay of ABCB5 & FKBP51 levels. (b) FKBP51 overexpression enhances ABCB5 levels. (Left) Normalized expression of FKBP51 mRNA (black) & ABCB5 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=3. (Left) Western blot assay of ABCB5 & FKBP51 levels in the same cells. Anti-Flag-labelled exogenous FKBP51. (c) ABCG2+ melanoma cells increase in FKBP51-overexpressing cells. (Upper) Normalized expression of FKBP51 mRNA (black) & ABCG2 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=5. (Lower) Flow cytometric histograms of ABCG2 expression (green population); mean±S.D. of counts are shown. (d) Enhanced FKBP51 mRNA levels in sorted ABCG2+ melanoma cells (SAN, upper; A375, lower). Whole cell expression=1; (n=3). (e) Enhanced EMT gene transcripts in sorted ABCG2+ melanoma cells. ABCG2− sample expression=1; n=3. (f) Expression of ABCG2 transcript in 9 deparaffinized tumours, 4 primary melanoma (M) samples, & 5 metastases (MM). A naevus sample was arbitrarily chosen with expression=1 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68240_rabbit-polyclonal-fkbp51-fkbp5-antibody-31020241612662.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) & ABCB5 mRNA (grey) (n=3). FKBP51-treated sample expression=1. (Right) Western blot assay of ABCB5 & FKBP51 levels. (b) FKBP51 overexpression enhances ABCB5 levels. (Left) Normalized expression of FKBP51 mRNA (black) & ABCB5 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=3. (Left) Western blot assay of ABCB5 & FKBP51 levels in the same cells. Anti-Flag-labelled exogenous FKBP51. (c) ABCG2+ melanoma cells increase in FKBP51-overexpressing cells. (Upper) Normalized expression of FKBP51 mRNA (black) & ABCG2 mRNA (grey) measured in WT, EV-, or FKBP51-stably transfected melanoma cells. WT sample expression=1; n=5. (Lower) Flow cytometric histograms of ABCG2 expression (green population); mean±S.D. of counts are shown. (d) Enhanced FKBP51 mRNA levels in sorted ABCG2+ melanoma cells (SAN, upper; A375, lower). Whole cell expression=1; (n=3). (e) Enhanced EMT gene transcripts in sorted ABCG2+ melanoma cells. ABCG2− sample expression=1; n=3. (f) Expression of ABCG2 transcript in 9 deparaffinized tumours, 4 primary melanoma (M) samples, & 5 metastases (MM). A naevus sample was arbitrarily chosen with expression=1 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68240_rabbit-polyclonal-fkbp51-fkbp5-antibody-310202415535195.jpg)
![Western Blot: FKBP51/FKBP5 Antibody [NB100-68240] - FKBP51 interacts with the general transcriptional co-activator p300. (a, left) FKBP51 co-immunoprecipitates with p300. (a, right), p300 co-immunoprecipitates with FKBP51. Total-cell lysates were prepared by SAN melanoma cells transfected with FKBP51/Flag. Cell lysates were immunoprecipitated with anti-Kat3B/p300 (IP p300) or anti-Flag (IP FKBP51). Immunoprecipitated & total lysates were then subjected to western blot with anti-FKBP51 or anti-p300. (b) ChIP performed with SAN melanoma cells, silenced (FKBP51 siRNA) or not (NS RNA) for FKBP51. An enrichment of DNA (region at −3450 from TSS of ABCG2 gene) can be observed in p300-immunoprecipitated chromatin (NS RNA) compared with IgG sample. Such an enrichment appeared to be reduced when FKBP51 was silenced (FKBP51 siRNA) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68240_rabbit-polyclonal-fkbp51-fkbp5-antibody-310202415532018.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody [NB110-17780] - Immunohistochemical staining with NB110-17780, anti- AG-2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.](http://images.novusbio.com/fullsize/AG-2-Antibody-Immunohistochemistry-Paraffin-NB110-17780-img0005.jpg "Immunohistochemistry-Paraffin: AG-2 Antibody [NB110-17780] - Immunohistochemical staining with NB110-17780, anti- AG-2 antibody, in formalin-fixed, paraffin-embedded normal colon tissue at 1:100 dilution.")
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-31020241534351.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-310202415392510.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-310202415384145.jpg)
![Immunohistochemistry: AG-2/AGR2 Antibody - BSA Free [NB110-17780] - SATB1 immunohistochemistry staining. A, benign prostate hyperplasia. B, prostatic carcinoma with metastasis. C, prostatic carcinoma without metastasis. D, negative control. Magnification: 400 × . Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23642278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-17780_rabbit-polyclonal-ag-2-agr2-antibody-31020241517528.jpg)
![Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/TEM8-ANTXR1-Antibody-200C1339-SB20-Western-Blot-NB100-56585-img0018.jpg "Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.")
![Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.](http://images.novusbio.com/fullsize/TEM8-ANTXR1-Antibody-200C1339-SB20-Immunohistochemistry-Paraffin-NB100-56585-img0016.jpg)
![Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).](http://images.novusbio.com/fullsize/TEM8-ANTXR1-Antibody-200C1339-SB20-Western-Blot-NB100-56585-img0017.jpg)
![Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)](http://images.novusbio.com/fullsize/TEM8-ANTXR1-Antibody-200C1339-SB20-Immunohistochemistry-Paraffin-NB100-56585-img0015.jpg)
![Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36431) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Knockout-Validated-NBP2-36431-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36431) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 recombinant protein with S100A4 antibody (clone 1C4) at 0.5 ug/ml concentration.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Western-Blot-NBP2-36431-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1C4) [NBP2-36431] - S100A4 (1C4) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunocytochemistry-Immunofluorescence-NBP2-36431-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0009.jpg)
![Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on THP-1 cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Flow-Cytometry-NBP2-36431-img0012.jpg)
![Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 in lysates of A375 and A549 cell lines with S100A4 antibody (clone 1C4). The antibody detected the endogenous protein at its expected molecular weight position 11.7 kDa.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Western-Blot-NBP2-36431-img0003.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, macrophages) developed nuclear staining also.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0005.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Aanalysis of tissue section of Squamous Cell Carcinoma of Human Lungs with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. An expected cytoplasmic as well as nuclear staining was observed in the tissue and the staining was found to be more intense in several cells which are potentially the advanced cancer cells, lymphocytes or infiltrating macrophages.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0006.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human colon with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The columnar epithelial cells showed weak to negligible immunopositivity whereas the cells present in lamina propria, the loose cellular connective tissue in mucosa, developed intense staining in various type of cells.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human skeletal muscle with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The different myocytes showed a varying degree of cytoplasmic immuno-positivity whereas the staining was found to be intense in some of the peripherally placed nuclei.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0008.jpg)
![Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on HeLa cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Flow-Cytometry-NBP2-36431-img0011.jpg)
![Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Western-Blot-NBP2-24529-img0001.jpg "Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Immunohistochemistry-Paraffin-NBP2-24529-img0002.jpg)
![Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Simple-Western-NBP2-24529-img0003.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.3). Formalin-paraffin human Angiosarcoma.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Immunohistochemistry-Frozen-NBP2-15202-img0013.jpg "Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.3). Formalin-paraffin human Angiosarcoma.")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using the Azide and BSA Free version of CD31/PECAM-1 Antibody (C31.3). Staining of human tonsil.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Immunohistochemistry-Paraffin-NBP2-15202-img0012.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.3).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Western-Blot-NBP2-15202-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with CD31/PECAM-1 Antibody (C31.3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Immunocytochemistry-Immunofluorescence-NBP2-15202-img0015.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Flow Cytometric analysis of human CD31 on Jurkat cells. Black: cells alone; grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.3).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-Flow-Cytometry-NBP2-15202-img0014.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Staining with CD31/PECAM-1 Antibody (C31.3) [Alexa Fluor® 647] (NBP2-33154AF647) using GE Cell DIVE (20x) on Human Rheumatoid Arthritis synovium (10 ug/ml). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-15202_mouse-monoclonal-cd31-pecam-1-antibody-c31-3-17720241044649.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Western-Blot-NBP2-36429-img0018.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunocytochemistry-Immunofluorescence-NBP2-36429-img0012.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0017.jpg)
![Flow (Cell Surface): VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Flow-Cell-Surface-NBP2-36429-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0013.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0014.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0015.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0016.jpg)
![Western Blot: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, Hep-G2, K-562, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BARD1 antibody NB100-319 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BARD1-Antibody-Western-Blot-NB100-319-img0016.jpg "Western Blot: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, Hep-G2, K-562, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BARD1 antibody NB100-319 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BARD1 antibody NB100-319 (NB100-319 lot 4) used for IP at 6 ug per reaction. BARD1 was also immunoprecipitated by a previous lot of this antibody (NB100-319 lot 3). For blotting immunoprecipitated BARD1, NB100-319 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BARD1-Antibody-Immunoprecipitation-NB100-319-img0015.jpg)
![Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.](http://images.novusbio.com/fullsize/Rad9-Antibody-93A535-Western-Blot-NB120-13600-img0005.jpg "Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.")
![Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) [NB120-13600] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Rad9-Antibody-93A535-Immunohistochemistry-Paraffin-NB120-13600-img0006.jpg)