| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76377-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71753 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-77452SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78964SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44807 | |||
| Azide and BSA Free | NBP2-34779-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70741 | |||
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Western Blot: IRGM Antibody [NBP1-76377] - Analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/IRGM-Antibody-Western-Blot-NBP1-76377-img0005.jpg "Western Blot: IRGM Antibody [NBP1-76377] - Analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: IRGM Antibody [NBP1-76377] - Staining of CD4 in human thymus tissue with CD4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunohistochemistry-Paraffin-NBP1-76377-img0003.jpg)
![Immunohistochemistry: IRGM Antibody [NBP1-76377] - Staining of IRGM in Human Brain tissue with IRGM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunofluorescence-NBP1-76377-img0002.jpg)
![Immunohistochemistry: IRGM Antibody - BSA Free [NBP1-76377] - Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76377_rabbit-polyclonal-irgm-antibody-2711202420141919.gif)
![Immunohistochemistry: IRGM Antibody - BSA Free [NBP1-76377] - Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76377_rabbit-polyclonal-irgm-antibody-2711202420195388.gif)
![Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Western-Blot-NBP2-02477-img0026.jpg "Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunocytochemistry-Immunofluorescence-NBP2-02477-img0016.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0023.jpg)
![Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Flow-Cytometry-NBP2-02477-img0015.jpg)
![Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP Kinase 2.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Western-Blot-NBP2-02477-img0024.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0017.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0018.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human colon tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0019.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human Kidney tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0020.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human liver tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0021.jpg)
![Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human lung tissue using anti-DAP Kinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-02477-img0022.jpg)
![Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Flow-Cytometry-NBP2-02477-img0014.jpg)
![Immunoprecipitation: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/DAP-Kinase-2-Antibody-OTI1C5-Immunoprecipitation-NBP2-02477-img0027.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419592087.jpg "Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Mouse Testis.Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202420312999.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Human Testis.Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Nanog antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241932686.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in HeLa Cells.Immunofluorescent analysis of PFA-fixed HeLa cells labeling Nanog with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419553033.jpg)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in Mouse and Rat Testis.Antibodies: Nanog, 2 u/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241923639.gif)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in HeLa CellsLoading: 15 u of lysate. Antibodies: Nanog, 2 u/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419463442.gif)
![Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-77452-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-77452] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of TSG101 antibody (NBP2-77452). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the cytoplasm and membrane of glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-77452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0003.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-78964-img0015.jpg "Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.")
![Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-78964] - IF Confocal analysis of A549 cells using LC3I antibody (NBP1-78964, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78964-img0017.jpg)
![Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-NBP1-78964-img0016.jpg)
![Western Blot: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Chloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Chloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.")
![<b>Genetic Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0002.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NBP2-59703] - IHC (Immunohistochemical) analysis of a formalin fixed and paraffin embedded tissue section of normal mouse brain using rabbit monoclonal LC3B (1251A) antibody [Catalog # NBP2-46892] at 1:100 dilution with HRP-DAB detection. The antibody generated a weak diffused cytoplasmic staining in most of the cells but some cells, especially within empty areas on the section, showed punctate signal also which signifies the presence of autophagy in those areas.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NBP2-59703-img0005.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NBP2-59703] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0001.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NBP2-59703] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0003.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (SPM524) [NBP2-32943] - Formalin-fixed, paraffin-embedded human prostate stained with CD63 Mouse Monoclonal Antibody (SPM524) (DAB chromogen).](http://images.novusbio.com/fullsize/CD63-Antibody-SPM524-Immunohistochemistry-Paraffin-NBP2-32943-img0007.jpg "Immunohistochemistry-Paraffin: CD63 Antibody (SPM524) [NBP2-32943] - Formalin-fixed, paraffin-embedded human prostate stained with CD63 Mouse Monoclonal Antibody (SPM524) (DAB chromogen).")
![Flow Cytometry: CD63 Antibody (SPM524) [NBP2-32943] - Flow Cytometric staining of human CD63 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (SPM524).](http://images.novusbio.com/fullsize/CD63-Antibody-SPM524-Flow-Cytometry-NBP2-32943-img0006.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (SPM524) [NBP2-32943] - Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Antibody (SPM524) (AEC Chromogen)](http://images.novusbio.com/fullsize/CD63-Antibody-SPM524-Immunohistochemistry-Paraffin-NBP2-32943-img0004.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (SPM524) [NBP2-32943] - Formalin-paraffin mouse spleen stained with CD63 MAb (SPM524)](http://images.novusbio.com/fullsize/nbp2-32943_mouse-monoclonal-cd63-antibody-spm524-1772024104460.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0005.jpg "Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-326). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-326-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0002.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0003.jpg)
![Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-326-img0001.jpg)
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg)
![Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Sandwich-ELISA-H00026330-M01-img0008.jpg)
![Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Western-Blot-NBP2-02093-img0002.jpg "Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.")
![Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] Staining of paraffin-embedded Human Kidney tissue using anti-FKBP12 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Immunohistochemistry-Paraffin-NBP2-02093-img0004.jpg)
![Flow Cytometry: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FKBP12 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Flow-Cytometry-NBP2-02093-img0001.jpg)
![Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FKBP12 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Immunohistochemistry-Paraffin-NBP2-02093-img0003.jpg)