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![Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24821-img0001.jpg "Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.")
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NBP2-24821] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-TLR4 [NBP2-24821] at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24821-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 in FFPE (A) normal and (B) tumor prostate sections using TLR4 antibody at 1:50 dilution. ICC analysis of TLR4 in formalin-fixed (C) Ramos and (D) 293 cells using antibody at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NBP2-24821-img0002.jpg)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24821. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (green). Novus's TLR cell surface flow kit (10099K) was used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0005.jpg)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Cell surface analysis using TLR4 antibody. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control antibody (green). This antibody and PE-conjugated secondary were used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0003.jpg)
![Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Western-Blot-NB300-129-img0009.jpg "Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Analysis of HeLa cells stained with rabbit pAb to ubiquitin, NB300-129, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0008.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB300-129] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunohistochemistry-Paraffin-NB300-129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0001.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56101-img0002.jpg "Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.")
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG5 was detected using NB110-53818.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0022.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0015.jpg)
![Simple Western: Autophagy Antibody Pack [NB910-94159] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94159-img0029.jpg)
![Flow Cytometry: Autophagy Antibody Pack [NB910-94159] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94159-img0027.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0030.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0021.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0028.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0016.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0017.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG16L1. Lane 1: NIH/3T3 Lane 2: HeLa .](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0018.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0024.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0025.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0010.jpg "Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0005.jpg)
![Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunohistochemistry-NBP1-48321-img0006.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0007.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0009.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0005.jpg "Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - 10 ug of Chloroquine treated (+) and untreated (-) HeLa lysates in 1x Laemmli sample buffer (NBP2-49689) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0002.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - Human Cervical carcinoma cells (HeLa) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49689) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0003.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with vehicle (-) or with 50 uM chloriquine (+) for 24 hours and the total cell lysates were prepared. The lysates were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF mebbrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml of p62/SQSTM1 antibody (NBP1-48320) in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0004.jpg)
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg)
![Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg)
![Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0006.jpg "Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.")
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human breast carcinoma using AKT1 antibody at 1:50. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0010.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of extracts from COS cells using AKT1 antibody at 1:1000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human endometrial cancer using AKT1 antibody at 1:25. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0009.jpg)
![Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56579-img0002.jpg "Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).")
![Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0013.jpg "Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg)
![Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg)
![Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.](http://images.novusbio.com/fullsize/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg)
![Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/NF-M-Antibody-Knockout-Validated-NB300-133-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Analysis of rat cerebellum section stained with rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in red, and costained with mouse mAb to GAP43, dilution 1:2,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. NB300-133 antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regios in the molecular layer.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0008.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of neuronal tissue lysates using rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NB300-133-img0009.jpg)
![Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - Staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons. The green channel shows staining with a monoclonal antibody to beta-adrendergic receptor kinase 1.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Frozen-NB300-133-img0007.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.](http://images.novusbio.com/fullsize/p_165_1.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein closely related to Fox3/NeuN. The RPCA-NF-M antibody stains axonal, dendritic and perikaryal profiles of neurons cleanly and specifically. Like antibody to Fox3/NeuN, the Fox1 antibody binds to the nuclei of neurons only. DNA is shown in blue with the DAPI stain.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0005.jpg)