ZMPSTE24 Antibody

Western Blot: ZMPSTE24 Antibody [NB100-2387] - Detection of ZMPSTE24 in human testis.

Immunocytochemistry/Immunofluorescence: ZMPSTE24 Antibody [NB100-2387] - analysis of ZMPSTE24 in A549 cells using anti-ZMPSTE24 antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Ze, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ZMPSTE24 Antibody Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human ZMPSTE24 protein sequence (between residues 1-100). [Swiss-Prot O75844]
• Localization: Integral membrane, primarily ER
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZMPSTE24
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0-2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence
• Application Notes: This ZMPSTE24 antibody is useful for Western Blot, where a band is seen at ~54 kDa representing ZMPSTE24 protein.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  Testis Lysate (NB820-59672)
  Testis Lysate (NB820-59266)
  ZMPSTE24 Lysate (NBP2-65335)

• Control Peptide:

  ZMPSTE24 Peptide (NB100-2387PEP)

Reactivity Notes

Human, mouse and zebrafish. Predicted to react with primate based on 100% sequence homology.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZMPSTE24 Antibody

• zona pellucida binding protein 2
• zona pellucida-binding protein 2
• ZPBP-like protein
• ZPBPLMGC41930

Background

ZMPSTE24 (zinc metalloproteinase Ste24 homolog) is a zinc metalloprotease that catalyzes the removal of C-terminal three residues of farnesylated proteins and is essential for maturation of lamin A (a nuclear scaffold protein). ZMPSTE24 belongs to peptidase M48A family and is a membrane-associated enzyme which contains 7-transmembrane segments localized to ER membrane as well as inner nuclear membrane. ZMPSTE24 contains a consensus zinc metalloprotease motif, consisting of a HEXXH catalytic site, located in a loop predicted to reside in the cytosol/nucleoplasm and farnesylated substrates such as prelamin A are accessible to the HEXXH domain for proteolytic cleavage. Lamin A, together with lamin C and B-type lamins, form the meshwork that underlies the nuclear envelope, and provide structure/shape to the nucleus along with playing many critical roles in nuclear function such as binding of heterochromatin, regulation of transcription factors, positioning of nuclear pore complexes and facilitating response to DNA damage. Defects in ZMPSTE24 have been linked to mandibuloacral dysplasia with type B lipodystrophy (MADB) and lethal tight skin contracture syndrome (LTSCS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZMPSTE24 Antibody (NB100-2387)(2)

Publications using NB100-2387

• Ponnusamy S, Zhang H, Kadam P et al. Membrane proteins of human fetal primitive nucleated red blood cells J Proteomics 2012 Jul 25 [PMID: 22842289] (ICC/IF, Human)
• Coffinier, C et al. HIV protease inhibitors block the zinc metalloproteinase ZMPSTE24 and lead to an accumulation of prelamin A in cells. Proc Natl Acad Sci U S A;104(33):13432-7. 2007 Aug 14. [PMID: 17652517]

Review for ZMPSTE24 Antibody (NB100-2387) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IF Human 11/26/2014

Summary

• Application: Immunofluorescence
• Sample Tested: A549 lung epithelial cells
• Species: Human

Blocking

• Blocking Details: 1X PBS containing 5% normal serum

Primary Anitbody

• Dilution Ratio: 1:100 dilution and incubated at 4C for 12 hr.

Secondary Antibody

• Secondary Description: Alexa Fluor 594 Goat Anti-Rabbit IgG (H+L)
• Secondary Manufacturer Cat#: Life Technologies, #A11037
• Secondary Concentration: 1:200

Details

• Detection Notes: Images were captured and analyzed using Olympus Fluoview Confocal microscope (Olympus, Center Valley, PA)
• Fixation Details: Slides are treated with cold methanol at -20C for 10 min.
• Wash Description: Washed with 1X PBS for 3 times, each time for 5 min.

Comments

• Comments: Cells were cultured in the Lab-Tek II Chamber Slide w/Cover CC2 Glass Slide Sterile, 4-well (Thermo Fisher Scientific, Catalog #154917). After indicated treatment, cells were fixed and permeabilized using cold methanol for 10 min at -20°C. Then the slides were washed with 1X PBS for 10 min and blocked with 1XPBS containing 5% Normal Goat Serum for 1 hr. The slides were incubated diluted anti-ZMP antibody (1:100) at 4°C for 12hr.After 3X washings (5 min each wash) with 1X PBS, cells were incubated with corresponding Alexa Fluor conjugated secondary antibodies (Life Technologies) for 1 hr at room temperature. The slides were washed 3 times (5min each time) with 1X PBS and counter stained with 300nM DAPI for 1 min, followed by 1X PBS washing for 1 min. After air drying, slides were sealed with Gold Seal Cover Glass (Electron Microscopy Sciences, Catalog #3223) using Fluorogel (Electron Microscopy Sciences, Catalog #17985-10). Images were captured and analyzed using Olympus Fluoview Confocal microscope (Olympus, Center Valley, PA).

Bioinformatics

• Gene Symbol: ZMPSTE24