ZMPSTE24 Antibody

Images

 
Western Blot: ZMPSTE24 Antibody [NB100-2387] - Detection of ZMPSTE24 in human testis.
Immunocytochemistry/ Immunofluorescence: ZMPSTE24 Antibody [NB100-2387] - analysis of ZMPSTE24 in A549 cells using anti-ZMPSTE24 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Ze, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ZMPSTE24 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human ZMPSTE24 protein sequence (between residues 1-100). [Swiss-Prot O75844]
Localization
Integral membrane, primarily ER
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZMPSTE24
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0-2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
This ZMPSTE24 antibody is useful for Western Blot, where a band is seen at ~54 kDa representing ZMPSTE24 protein.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Testis Lysate (NB820-59672)
Testis Lysate (NB820-59266)
ZMPSTE24 Lysate (NBP2-65335)
Control Peptide
ZMPSTE24 Peptide (NB100-2387PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB100-2387 in the following applications:

Publications
Read Publications using
NB100-2387 in the following applications:

Reactivity Notes

Human, mouse and zebrafish. Predicted to react with primate based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZMPSTE24 Antibody

  • zona pellucida binding protein 2
  • zona pellucida-binding protein 2
  • ZPBP-like protein
  • ZPBPLMGC41930

Background

ZMPSTE24 (zinc metalloproteinase Ste24 homolog) is a zinc metalloprotease that catalyzes the removal of C-terminal three residues of farnesylated proteins and is essential for maturation of lamin A (a nuclear scaffold protein). ZMPSTE24 belongs to peptidase M48A family and is a membrane-associated enzyme which contains 7-transmembrane segments localized to ER membrane as well as inner nuclear membrane. ZMPSTE24 contains a consensus zinc metalloprotease motif, consisting of a HEXXH catalytic site, located in a loop predicted to reside in the cytosol/nucleoplasm and farnesylated substrates such as prelamin A are accessible to the HEXXH domain for proteolytic cleavage. Lamin A, together with lamin C and B-type lamins, form the meshwork that underlies the nuclear envelope, and provide structure/shape to the nucleus along with playing many critical roles in nuclear function such as binding of heterochromatin, regulation of transcription factors, positioning of nuclear pore complexes and facilitating response to DNA damage. Defects in ZMPSTE24 have been linked to mandibuloacral dysplasia with type B lipodystrophy (MADB) and lethal tight skin contracture syndrome (LTSCS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZMPSTE24 Antibody (NB100-2387)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
(1)
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Review for ZMPSTE24 Antibody (NB100-2387) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2387:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence ZMPSTE24 NB100-2387
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reviewed by:
Anonymous
IF Human 11/26/2014
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Summary

ApplicationImmunofluorescence
Sample TestedA549 lung epithelial cells
SpeciesHuman

Blocking

Blocking Details1X PBS containing 5% normal serum

Primary Anitbody

Dilution Ratio1:100 dilution and incubated at 4C for 12 hr.

Secondary Antibody

Secondary DescriptionAlexa Fluor 594 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#Life Technologies, #A11037
Secondary Concentration1:200

Details

Detection NotesImages were captured and analyzed using Olympus Fluoview Confocal microscope (Olympus, Center Valley, PA)
Fixation DetailsSlides are treated with cold methanol at -20C for 10 min.
Wash DescriptionWashed with 1X PBS for 3 times, each time for 5 min.

Comments

CommentsCells were cultured in the Lab-Tek II Chamber Slide w/Cover CC2 Glass Slide Sterile, 4-well (Thermo Fisher Scientific, Catalog #154917). After indicated treatment, cells were fixed and permeabilized using cold methanol for 10 min at -20°C. Then the slides were washed with 1X PBS for 10 min and blocked with 1XPBS containing 5% Normal Goat Serum for 1 hr. The slides were incubated diluted anti-ZMP antibody (1:100) at 4°C for 12hr.After 3X washings (5 min each wash) with 1X PBS, cells were incubated with corresponding Alexa Fluor conjugated secondary antibodies (Life Technologies) for 1 hr at room temperature. The slides were washed 3 times (5min each time) with 1X PBS and counter stained with 300nM DAPI for 1 min, followed by 1X PBS washing for 1 min. After air drying, slides were sealed with Gold Seal Cover Glass (Electron Microscopy Sciences, Catalog #3223) using Fluorogel (Electron Microscopy Sciences, Catalog #17985-10). Images were captured and analyzed using Olympus Fluoview Confocal microscope (Olympus, Center Valley, PA).

Product General Protocols

View specific protocols for ZMPSTE24 Antibody (NB100-2387): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZMPSTE24 Antibody (NB100-2387) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2387AF405
Alexa Fluor 488 Labeled NB100-2387AF488
Alexa Fluor 647 Labeled NB100-2387AF647
Alexa Fluor 700 Labeled NB100-2387AF700
Biotin Labeled NB100-2387B
DyLight 405 Labeled NB100-2387V
DyLight 488 Labeled NB100-2387G
DyLight 550 Labeled NB100-2387R
DyLight 650 Labeled NB100-2387C
FITC Labeled NB100-2387F
HRP Labeled NB100-2387H

Additional Array Products

Bioinformatics Tool for ZMPSTE24 Antibody (NB100-2387)

Discover related pathways, diseases and genes to ZMPSTE24 Antibody (NB100-2387). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ZMPSTE24 Antibody (NB100-2387)

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Research Areas for ZMPSTE24 Antibody (NB100-2387)

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Blogs on ZMPSTE24.

ZMPSTE24 Mutations, Lamin A Processing & Laminopathies
ZMPSTE24 (FACE-1, CAAX prenyl protease 1 homolog) is a membrane associated zinc metalloprotease of the peptidase M48A family. It's catalytic activity can be defined as the peptide bond hydrolyzed in the sequence -C-|-A-A-X in which C is an S-isoprenyl...  Read full blog post.

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Recent Reviews

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Anonymous
11/26/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol ZMPSTE24