ZDHHC8 Antibody

Western Blot: ZDHHC8 Antibody [NB100-57849] - (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZDHHC8 Antibody Summary

• Description: Novus Biologicals Goat ZDHHC8 Antibody (NB100-57849) is a polyclonal antibody validated for use in WB and ELISA. Anti-ZDHHC8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QRDHPQLKTPPSK corresponding to internal region according to NP_037505.1.
• Epitope: QRDHPQLKTPPSK
• Specificity: This antibody is expected to recognise isoform 2 (NP_037505.1) only.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZDHHC8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 80-85 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 81.4 kDa band according to NP_037505.1). An additional band of 48 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
• Control:

  Human Brain Frontal Lobe Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZDHHC8 Antibody

• DHHC8
• EC 2.3.1
• EC 2.3.1.-
• KIAA1292DHHC-8
• membrane-associated DHHC8 zinc finger protein
• probable palmitoyltransferase ZDHHC8
• ZDHHCL1
• Zinc finger DHHC domain-containing protein 8
• Zinc finger protein 378
• zinc finger, DHHC-type containing 8
• ZNF378zinc finger, DHHC domain like containing 1

Background

The gene ZDHHC8, which encodes a putative palmitoyltransferase at 22q11, has been proposed as a gene which may increase liability to schizophrenia. Chromosome region 22q11 has been proposed as a major candidate locus for genes leading to susceptibility to schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZDHHC8 Antibody (NB100-57849)(1)

Publication using NB100-57849

• Mukai J, Liu H, Burt RA et al. Evidence that the gene encoding ZDHHC8 contributes to the risk of schizophrenia. Nat Genet 2004-07-01 [PMID: 15184899]

Bioinformatics

• Gene Symbol: ZDHHC8
• Entrez:
  • 29801(Human)
  • 27801(Mouse)
  • 303796(Rat)
• Uniprot:
  • Q9ULC8(Human)