ZDHHC8 Antibody

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Western Blot: ZDHHC8 Antibody [NB100-57849] - (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ZDHHC8 Antibody Summary

Immunogen
Peptide with sequence C-QRDHPQLKTPPSK corresponding to internal region according to NP_037505.1.
Epitope
QRDHPQLKTPPSK
Specificity
This antibody is expected to recognise isoform 2 (NP_037505.1) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZDHHC8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 80-85 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 81.4 kDa band according to NP_037505.1). An additional band of 48 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
Positive Control
Brain Frontal Lobe Lysate (NB820-59186)
Publications
Read Publication using NB100-57849.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZDHHC8 Antibody

  • DHHC8
  • EC 2.3.1
  • EC 2.3.1.-
  • KIAA1292DHHC-8
  • membrane-associated DHHC8 zinc finger protein
  • probable palmitoyltransferase ZDHHC8
  • ZDHHCL1
  • Zinc finger DHHC domain-containing protein 8
  • Zinc finger protein 378
  • zinc finger, DHHC-type containing 8
  • ZNF378zinc finger, DHHC domain like containing 1

Background

The gene ZDHHC8, which encodes a putative palmitoyltransferase at 22q11, has been proposed as a gene which may increase liability to schizophrenia. Chromosome region 22q11 has been proposed as a major candidate locus for genes leading to susceptibility to schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZDHHC8 Antibody (NB100-57849)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZDHHC8
Entrez
Uniprot