WDR4 Antibody (1F9) - Azide and BSA Free

Western Blot: WDR4 Antibody (1F9) [H00010785-M01] - WDR4 monoclonal antibody (M01), clone 1F9 Analysis of WDR4 expression in Hela S3 NE.

Immunohistochemistry-Paraffin: WDR4 Antibody (1F9) [H00010785-M01] - Analysis of monoclonal antibody to WDR4 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1 ug/ml.

Western Blot: WDR4 Antibody (1F9) [H00010785-M01] - Analysis of WDR4 expression in transfected 293T cell line by WDR4 monoclonal antibody (M01), clone 1F9.Lane 1: WDR4 transfected lysate (Predicted MW: 45.5 KDa).Lane 2: Non-transfected lysate.

ELISA: WDR4 Antibody (1F9) [H00010785-M01] - Detection limit for recombinant GST tagged WDR4 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

WDR4 Antibody (1F9) - Azide and BSA Free Summary

• Immunogen: WDR4 (AAH01074, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLLDVAVSPDDRFILTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLWEYRSGRQLHCCHLASLQELVDPQAPQKFAASRIAFWCQENCVALLCDGTSVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGSAGADASFSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRRSPPPGPDGHAKKMRPGEATLSC
• Specificity: WDR4 - WD repeat domain 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: WDR4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WDR4 Antibody (1F9) - Azide and BSA Free

• tRNA (guanine-N(7)-)-methyltransferase subunit WDR4
• WD repeat domain 4, 10TRM82
• WD repeat-containing protein 4

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR4
• Entrez:
  • 10785(Human)
• OMIM:
  • 605924(Human)
• Uniprot:
  • AAH01074(Human)