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VSTM1 Antibody (01)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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VSTM1 Antibody (01) Summary

Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human VSTM1 (Q6UX27-16; Met1-Thr135).
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
VSTM1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for VSTM1 Antibody (01)

  • LAIR Homolog
  • MGC119160
  • MGC119161
  • OSCAR-Like Transcript-1
  • signal inhibitory receptor on leukocytes-1
  • SIRL1
  • UNQ3033
  • V-set and transmembrane domain containing 1
  • V-set and transmembrane domain-containing protein 1
  • VSTM1
  • VSTM1-v1
  • VSTM1-v2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Bioinformatics

Gene Symbol VSTM1