VPS18 Antibody (2G10) - Azide and BSA Free

Western Blot: VPS18 Antibody (2G10) [H00057617-M02] - VPS18 monoclonal antibody (M02), clone 2G10. Analysis of VPS18 expression in Raw 264.7.

Sandwich ELISA: VPS18 Antibody (2G10) [H00057617-M02] - Detection limit for recombinant GST tagged VPS18 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 2G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VPS18 Antibody (2G10) - Azide and BSA Free Summary

• Immunogen: VPS18 (NP_065908, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKV
• Specificity: VPS18 (2G10)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VPS18
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VPS18 Antibody (2G10) - Azide and BSA Free

• hVPS18
• KIAA1475PEP3
• vacuolar protein sorting 18 homolog (S. cerevisiae)
• vacuolar protein sorting protein 18
• vacuolar protein sorting-associated protein 18 homolog

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS18 Antibody (H00057617-M02)(1)

Publication using H00057617-M02

• Tomita Y, Noda T, Fujii K et al. The cellular factors Vps18 and Mon2 are required for efficient production of infectious HIV-1 particles. J Virol. 2011-03-30 [PMID: 21450827]

Bioinformatics

• Gene Symbol: VPS18
• Entrez:
  • 57617(Human)
• OMIM:
  • 608551(Human)
• Uniprot:
  • NP_065908(Human)