USP28 Antibody

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Immunohistochemistry-Paraffin: USP28 Antibody [NB100-96911] - 3.8ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

USP28 Antibody Summary

Immunogen
Peptide with sequence C-SVELKHYIQEDN corresponding to internal region according to NP_065937.1.
Epitope
SVELKHYIQEDN
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
USP28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band and 50 kDa band in human bone marrow lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122 kDa band according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human testis shows acrosomal staining in spermatids.
Publications
Read Publication using NB100-96911.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP28 Antibody

  • Deubiquitinating enzyme 28
  • EC 3.1.2.15
  • EC 3.4.19.12
  • KIAA1515ubiquitin carboxyl-terminal hydrolase 28
  • ubiquitin specific peptidase 28
  • ubiquitin specific protease 28
  • ubiquitin thioesterase 28
  • Ubiquitin thiolesterase 28
  • Ubiquitin-specific-processing protease 28
  • USP28

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP28 Antibody (NB100-96911)(1)


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP28
Entrez
Uniprot