Recombinant Human Use1/UBE2Z Protein Summary
| Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-231 of Human Use1/UBE2Z Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAASRLE LNLVRLLSRC EAMAAEKRDP DEWRLEKYVG ALEDMLQALK VHASKPASEV INEYSWKVDF LKGMLQAEKL TSSSEKALAN QFLAPGRVPT TARERVPATK TVHLQSRARY TSEMRSELLG TDSAEPEMDV RKRTGVAGSQ PVSEKQSAAE LDLVLQRHQN LQEKLAEEML GLARSLKTNT LAAQSVIKKD NQTLSHSLKM ADQNLEKLKT ESERLEQHTQ KSVN |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
USE1 |
| Purity |
>80%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
28.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 1 mM DTT |
| Preservative |
No Preservative |
| Concentration |
0.25 mg/ml |
| Purity |
>80%, by SDS-PAGE |
Alternate Names for Recombinant Human Use1/UBE2Z Protein
Background
USE1, as known as P31, is a vesicle transport protein. This protein is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. It interacts directly with STX18. SNARE may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Recombinant human USE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 1 year from date of receipt.
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