Recombinant Human Uridine Phosphorylase 1/UPP1 Protein

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SDS-Page: Recombinant Human Uridine Phosphorylase 1/UPP1 Protein [NBP1-48364] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human Uridine Phosphorylase 1/UPP1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-310 of Human Uridine Phosphorylase 1/UPP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF GDVKFVCVGG SPSRMKAFIR CVGAELGLDC PGRDYPNICA GTDRYAMYKV GPVLSVSHGM GIPSISIMLH ELIKLLYYAR CSNVTIIRIG TSGGIGLEPG TVVITEQAVD TCFKAEFEQI VLGKRVIRKT DLNKKLVQEL LLCSAELSEF TTVVGNTMCT LDFYEGQGRL DGALCSYTEK DKQAYLEAAY AAGVRNIEME SSVFAAMCSA CGLQAAVVCV TLLNRLEGDQ ISSPRNVLSE YQQRPQRLVS YFIKKKLSKA

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UPP1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 200mM NaCl
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Uridine Phosphorylase 1/UPP1 Protein

  • EC 2.4.2.3
  • UDPRASE
  • UDRPASE
  • UP
  • UPASE 1
  • UPASE
  • UPP
  • UPP1
  • UPUPase 1
  • UrdPase 1
  • uridine phosphorylase 1
  • uridine phosphorylase

Background

Uridine phosphorylase 1, also known as UPP1 catalyses the reversible phosphorolysis of uridine to uracil. The reaction products are then utilized as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis. The expression levels and the enzymatic activity of UPP1 are higher in human solid tumors than in adjacent normal tissues. In addition, UPP1 controls the homeostatic regulation of uridine concentration in plasma and tissues and plays a role in the intracellular activation of 5-fluorouracil. Recombinant human UPP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UPP1
Entrez