UCMA Overexpression Lysate (Native)

Images

 
Western Blot: UCMA Overexpression Lysate (Adult Normal) [NBL1-08078] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UCMA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

UCMA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205431 and based on accession number NM_145314. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 10 open reading frame 49 (C10orf49), mRNA.
Gene
UCMA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for UCMA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UCMA Overexpression Lysate (Native)

  • C10orf49
  • chromosome 10 open reading frame 49
  • Gla-rich protein
  • GRP
  • unique cartilage matrix-associated protein
  • upper zone of growth plate and cartilage matrix associated

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-30061
Species: Hu
Applications: WB, ICC/IF, IHC-P
NBP1-05163
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-46576
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P
NBP1-35641
Species: Hu
Applications: WB, IHC, IHC-P
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
NBP1-46535
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, IHC, IHC-Fr, IHC-P
MAB2358
Species: Hu, Mu
Applications: IHC, ICC
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
AF2330
Species: Mu
Applications: WB, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP1-05164
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
NB300-201
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00008767-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA
NB300-619
Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for UCMA Lysate (NBL1-08078) (0)

There are no publications for UCMA Lysate (NBL1-08078).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for UCMA Lysate (NBL1-08078) (0)

There are no reviews for UCMA Lysate (NBL1-08078). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UCMA Lysate (NBL1-08078) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional UCMA Products

Bioinformatics Tool for UCMA Lysate (NBL1-08078)

Discover related pathways, diseases and genes to UCMA Lysate (NBL1-08078). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UCMA Lysate (NBL1-08078)

Discover more about diseases related to UCMA Lysate (NBL1-08078).
 

Pathways for UCMA Lysate (NBL1-08078)

View related products by pathway.

PTMs for UCMA Lysate (NBL1-08078)

Learn more about PTMs related to UCMA Lysate (NBL1-08078).

Blogs on UCMA

There are no specific blogs for UCMA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

UCMA Antibody
NBP2-33469

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our UCMA Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol UCMA