Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62)

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Western blot analysis of Ubiquitin-activating Enzyme/UBE1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature

Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of K562 cells with UBA1 antibody at 1:50 (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Whole spinal cord extracts from P8 mice. UBE1 band can be seen at 130 kDa. Antibody at 1:500. Image from verified customer review.

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Human skin fibroblast cell lysate, 5 ug total protein. Primary antibody at 1:500. Image from verified customer review.

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1:5000.

Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Staining UBA1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.

Product Discontinued

Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Ubiquitin-activating Enzyme/UBE1 aa 1,009-1,058 / 1,058. (SwissProt: P22314 Human; SwissProt: Q02053 Mouse; SwissProt: Q5U300 Rat)
• Localization: Nucleus, Mitochondrion, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62)

• A1S9T and BN75 temperature sensitivity complementing
• A1S9T
• A1ST
• AMCX1
• GXP1
• MGC4781
• POC20 centriolar protein homolog
• POC20
• Protein A1S9
• SMAX2
• UBA1
• UBA1, ubiquitin-activating enzyme E1 homolog A
• UBA1A
• UBE1
• UBE1A1S9
• UBE1X
• ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
• Ubiquitin-activating enzyme E1
• Ubiquitinactivating Enzyme
• Ubiquitin-activating Enzyme
• ubiquitin-like modifier activating enzyme 1
• ubiquitin-like modifier-activating enzyme 1

Background

E1 Ubiquitin Activating Enzyme is encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Ubiquitin-activating Enzyme/UBE1 Antibody (NBP2-67816) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Darija Soltic WB Human 01/07/2019

Summary

• Application: Western Blot
• Sample Tested: fibroblasts
• Species: Human
• Lot: HJ1017

Comments

• Comments: 5 ug of protein loaded
  Primary antibody: 1/500, one hour
  Secondary antibody: 1/1000, one hour

reviewed by: Darija Soltic WB Mouse 01/07/2019

Summary

• Application: Western Blot
• Sample Tested: Spinal cord
• Species: Mouse
• Lot: HJ1017

Comments

• Comments: Whole spinal cord extracts from P8 mice.
  Blocking: 4% powdered milk/PBS, 1 hour
  Primary antibody: 1/500, 1 hour
  Secondary antibody: HRP-labelled goat anti rabbit IgG, 1 hour

Bioinformatics

• Gene Symbol: UBA1