Ubiquitin-activating Enzyme/UBE1 Antibody (2G2.3)

Product Discontinued

Ubiquitin-activating Enzyme/UBE1 Antibody (2G2.3) Summary

• Immunogen: Ubiquitin activating enzyme E1.
• Specificity: The antibody specifically binds to ubiquitin activation enzyme E1. The antibody does not inhibit the enzymatic activity of E1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2-4 ug/ml
• Application Notes: Immunoprecipitation Sero-depletion

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 20mM Phosphate (pH 7.6) and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_003334.2;NM_153280.1

Alternate Names for Ubiquitin-activating Enzyme/UBE1 Antibody (2G2.3)

• A1S9T and BN75 temperature sensitivity complementing
• A1S9T
• A1ST
• AMCX1
• GXP1
• MGC4781
• POC20 centriolar protein homolog
• POC20
• Protein A1S9
• SMAX2
• UBA1
• UBA1, ubiquitin-activating enzyme E1 homolog A
• UBA1A
• UBE1
• UBE1A1S9
• UBE1X
• ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
• Ubiquitin-activating enzyme E1
• Ubiquitinactivating Enzyme
• Ubiquitin-activating Enzyme
• ubiquitin-like modifier activating enzyme 1
• ubiquitin-like modifier-activating enzyme 1

Background

The proteosome has long been thought of as the default mechanism for waste elimination within the cell. Recently, however, the proteosome has been recognized as an essential organelle necessary for the processing of antigens, the transcription factor NFkB and other proteins as well. Central to the function of the proteosome is the ubiquitinization of target proteins by a number of unique ubiquitin activation enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA1
• Uniprot:
  • P22314(Human)