Recombinant Human UBE2W Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human UBE2W Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-151 of Human UBE2W

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UBE2W
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human UBE2W Protein

  • EC 6.3.2.19
  • FLJ11011
  • hUBC-16
  • probable ubiquitin-conjugating enzyme E2 W
  • Ubc16
  • UBE2W
  • Ubiquitin carrier protein W
  • ubiquitin-conjugating enzyme 16
  • ubiquitin-conjugating enzyme E2W (putative)
  • Ubiquitin-protein ligase W

Background

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. This protein in vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is widely expressed, especially at highest levels in testis. Recombinant human UBE2W protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2W
Entrez