Recombinant Human UBE2W Protein

Product Discontinued

Recombinant Human UBE2W Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-151 of Human UBE2W
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2W
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human UBE2W Protein

• EC 6.3.2.19
• FLJ11011
• hUBC-16
• probable ubiquitin-conjugating enzyme E2 W
• Ubc16
• UBE2W
• Ubiquitin carrier protein W
• ubiquitin-conjugating enzyme 16
• ubiquitin-conjugating enzyme E2W (putative)
• Ubiquitin-protein ligase W

Background

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. This protein in vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is widely expressed, especially at highest levels in testis. Recombinant human UBE2W protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2W