UBE2N/Ubc13 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

UBE2N/Ubc13 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human UBE2N/Ubc13
  Met1-Ile172
  Accession # P61088
• Specificity: Detects human, rat, and mouse UBE2N/Ubc13 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human UbcH5b is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UBE2N/Ubc13 Antibody [Alexa Fluor® 700]

• bendless-like ubiquitin conjugating enzyme
• Bendless-like ubiquitin-conjugating enzyme
• BLU
• EC 6.3.2.19
• MGC131857
• MGC8489
• UBC13
• UbcH13
• UbcH-ben
• UBE2N
• Ubiquitin carrier protein N
• ubiquitin-conjugating enzyme E2 N
• ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
• ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Ubiquitin-protein ligase N
• yeast UBC13 homolog

Background

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a 16 kDa member of the Ubiquitin-conjugating (E2) enzyme family.  UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin.  UBE2N/Ubc13 localizes to both the nucleus and cytoplasm, and forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants.  The UBE2N (Ubc13)/Uev1A complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor kappa B (NF-kappa B) activation.  In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response.  Pathologically, UBE2N (Ubc13)/Uev1a complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells.  Human UBE2N/Ubc13 shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.