UBE2K/E2-25K Antibody

Western Blot: UBE2K/E2-25K Antibody [NB300-840] - (1.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: UBE2K/E2-25K Antibody [NB300-840] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6,

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UBE2K/E2-25K Antibody Summary

• Immunogen: Peptide with sequence C-SWDVETATELLLSN corresponding to C-Terminus according to NP_005330.1, NP_001104582.1, NP_001104583.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBE2K
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 24 kDa band observed in Jurkat lysates (calculated MW of 22.4 kDa band according to NP_005330). IHC-P: Human brain shows pixulate cytoplasm stainng in neurons of the cortex.
• Control:

  UBE2K/E2-25K Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBE2K/E2-25K Antibody

• E2(25K)
• E225K
• E2-25K
• EC 6.3.2.19
• Hip2
• HIP-2
• huntingtin interacting protein 2
• Huntingtin-interacting protein 2
• HYPG
• LIG
• UbcH1
• UBE2K
• UBE2K-25K
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 K
• Ubiquitin-conjugating enzyme E2(25K)
• Ubiquitin-conjugating enzyme E2-25 kDa
• Ubiquitin-conjugating enzyme E2-25K
• ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
• ubiquitin-protein ligase

Background

HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequent degradation of p53. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2K/E2-25K Antibody (NB300-840)(1)

Publication using NB300-840

• Kalchman MA, Graham RK, Xia G et al. Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J Biol Chem 1996-08-09 [PMID: 8702625]

Bioinformatics

• Gene Symbol: UBE2K
• Entrez:
  • 3093(Human)
• Uniprot:
  • NP_005330.1(Human)